##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
ApoI AscI AseI AsnI Asp718I
BamHI BbrPI BfrI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoT22I HincII
HindII HpaI KpnI MfeI MroI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SexAI SfuI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AgeI 5267 ACCGGT A^CCGGT
Aor51HI 954 AGCGCT AGC^GCT
AvrII 4867 CCTAGG C^CTAGG
BbeI 37 GGCGCC GGCGC^C
BcgI 3183 GCATGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1173 GGATAC GTATCC(6/5)
BclI 2335 TGATCA T^GATCA
BlnI 4867 CCTAGG C^CTAGG
BsiEI 2625 CGGCCG CG[GA][CT]^CG
BsiWI 1215 CGTACG C^GTACG
BsmI 4056 GCATTC GAATGC(1/-1)
BspHI 117 TCATGA T^CATGA
BspLU11I 1050 ACATGT A^CATGT
BssSI 3471 CACGAG CACGAG(-5/-1)
DrdI 1255 GACAGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2625 CGGCCG C^GGCCG
EclXI 2625 CGGCCG C^GGCCG
Eco47III 954 AGCGCT AGC^GCT
Eco52I 2625 CGGCCG C^GGCCG
EcoRV 3856 GATATC GAT^ATC
FbaI 2335 TGATCA T^GATCA
FseI 2622 GGCCGGCC GGCCGG^CC
KasI 37 GGCGCC G^GCGCC
MluI 315 ACGCGT A^CGCGT
NarI 37 GGCGCC GG^CGCC
NruI 2967 TCGCGA TCG^CGA
PinAI 5267 ACCGGT A^CCGGT
RcaI 117 TCATGA T^CATGA
SbfI 538 CCTGCAGG CCTGCA^GG
SfiI 4281 GGCCGTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 37 GGCGCC GGC^GCC
SgrAI 1861 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1455 GCATGC GCATG^C
Sse8387I 538 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 134 GACGTC GACGT^C
3845 GACGTC
AccI 2228 GTAGAC GT^[AC][GT]AC
2795 GTCTAC
AflIII 315 ACGCGT A^C[GA][CT]GT
1050 ACATGT
AhdI 1709 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5321 GACCATCTGTC
Alw44I 3065 GTGCAC G^TGCAC
4050 GTGCAC
ApaLI 3065 GTGCAC G^TGCAC
4050 GTGCAC
Asp700I 802 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
977 GAAGTGGTTC
AspEI 1709 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5321 GACCATCTGTC
BglII 2430 AGATCT A^GATCT
5160 AGATCT
BsaAI 1213 CACGTA [CT]AC^GT[GA]
1420 CACGTA
BsaBI 2336 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5318 GATGACCATC
Bsp1407I 1053 TGTACA T^GTACA
5351 TGTACA
BsrBI 1514 CCGCTC CCGCTC(-3/-3)
929 GAGCGG
BsrDI 1606 CATTGC GCAATG(2/0)
4015 CATTGC
BsrGI 1053 TGTACA T^GTACA
5351 TGTACA
DraI 2999 TTTAAA TTT^AAA
3315 TTTAAA
DraIII 3861 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
4589 CACCCCGTG
Eam1105I 1709 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5321 GACCATCTGTC
HindIII 4361 AAGCTT A^AGCTT
4411 AAGCTT
MamI 2336 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5318 GATGACCATC
SacI 1187 GAGCTC GAGCT^C
1411 GAGCTC
SmlI 1190 CTCAAG C^T[CT][GA]AG
2213 CTCAAG
SpeI 2672 ACTAGT A^CTAGT
5122 ACTAGT
SspBI 1053 TGTACA T^GTACA
5351 TGTACA
SspI 3319 AATATT AAT^ATT
5460 AATATT
XbaI 3342 TCTAGA T^CTAGA
5041 TCTAGA
XmnI 802 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
977 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI