##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AspI        AvrII       BbrPI       
BbvCI       BcgI        BglI        BlnI        BlpI        
Bpu1102I    BsaWI       BseAI       BsiEI       BsiWI       
BsmI        BspEI       BspHI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
CelII       Cfr10I      CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
FseI        KpnI        KspI        MluI        MroI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfiI        SfuI        SgrAI       Sse8387I    SwaI        
Tth111I     XcmI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         746  GACGTC               GACGT^C
AocI         1198  CCTAAGG              CC^T[ACGT]AGG
ApaI         1739  GGGCCC               GGGCC^C
AseI         3456  ATTAAT               AT^TAAT
AsnI         3456  ATTAAT               AT^TAAT
BamHI        2533  GGATCC               G^GATCC
BbeI          186  GGCGCC               GGCGC^C
BsaBI        1327  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1925  GAGACC               GGTCTC(1/5)
BspDI        1822  ATCGAT               AT^CGAT
BstEII       2249  GGTCACC              G^GT[ACGT]ACC
BstPI        2249  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1198  CCTAAGG              CC^T[ACGT]AGG
ClaI         1822  ATCGAT               AT^CGAT
Eco81I       1198  CCTAAGG              CC^T[ACGT]AGG
EcoNI        5170  CCTGATTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2249  GGTCACC              G^GT[ACGT]ACC
HapII         142  CCGG                 C^CGG
HpaII         142  CCGG                 C^CGG
KasI          186  GGCGCC               G^GCGCC
MamI         1327  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          596  CAATTG               C^AATTG
MspI          142  CCGG                 C^CGG
MunI          596  CAATTG               C^AATTG
NarI          186  GGCGCC               GG^CGCC
NcoI         4044  CCATGG               C^CATGG
PaeR7I       2759  CTCGAG               C^TCGAG
PflMI        2616  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3619  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3456  ATTAAT               AT^TAAT
SapI         1651  GCTCTTC              GCTCTTC(1/4)
SexAI        2209  ACCAGGT              A^CC[AT]GGT
SfoI          186  GGCGCC               GGC^GCC
SmaI          141  CCCGGG               CCC^GGG
SnaBI        1843  TACGTA               TAC^GTA
StuI         2272  AGGCCT               AGG^CCT
Van91I       2616  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2759  CTCGAG               C^TCGAG
XmaCI         141  CCCGGG               C^CCGGG
XmaI          141  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflII        2889  CTTAAG               C^TTAAG
             3247  CTTAAG              
AvaI          141  CCCGGG               C^[CT]CG[GA]G
             2759  CTCGAG              
AviII        2666  TGCGCA               TGC^GCA
             4610  TGCGCA              
BalI          936  TGGCCA               TGG^CCA
             2471  TGGCCA              
BanI          186  GGCGCC               G^G[CT][GA]CC
             5632  GGTGCC              
BclI         1332  TGATCA               T^GATCA
             2082  TGATCA              
BcnI          141  CCCGG                CC^[GC]GG
              142  CCGGG               
BfrI         2889  CTTAAG               C^TTAAG
             3247  CTTAAG              
BglII         975  AGATCT               A^GATCT
             2037  AGATCT              
BsaAI        1843  TACGTA               [CT]AC^GT[GA]
             3785  TACGTG              
BsoBI         141  CCCGGG               C^[CT]CG[GA]G
             2759  CTCGAG              
BspMI        5484  ACCTGC               ACCTGC(4/8)
             5651  GCAGGT              
BstXI         365  CCAATTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              452  CCATCAAGTTGG        
DsaI         3241  CCACGG               C^C[GA][CT]GG
             4044  CCATGG              
EaeI          936  TGGCCA               [CT]^GGCC[GA]
             2471  TGGCCA              
EcoRI        1447  GAATTC               G^AATTC
             3928  GAATTC              
EcoRV        3261  GATATC               GAT^ATC
             3640  GATATC              
FbaI         1332  TGATCA               T^GATCA
             2082  TGATCA              
FspI         2666  TGCGCA               TGC^GCA
             4610  TGCGCA              
HgaI          202  GCGTC                GACGC(5/10)
              208  GCGTC               
MluNI         936  TGGCCA               TGG^CCA
             2471  TGGCCA              
MscI          936  TGGCCA               TGG^CCA
             2471  TGGCCA              
NciI          141  CCCGG                CC^[GC]GG
              142  CCGGG               
PleI         1626  GAGTC                GAGTC(4/5)
             3912  GAGTC               
ScaI         1157  AGTACT               AGT^ACT
             3750  AGTACT              
SphI         2669  GCATGC               GCATG^C
             2764  GCATGC              
SspI         1234  AATATT               AAT^ATT
             4890  AATATT              
XbaI         2418  TCTAGA               T^CTAGA
             3615  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        ApoI        
Asp700I     AspHI       AvaII       BanII       BbsI        
BbvI        BciVI       BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    Bsp1407I    BspLU11I    BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MvnI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SpeI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI