##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        AspEI       BalI        BamHI       
BbrPI       BsaAI       BsaBI       BsmBI       BssHII      
BstBI       BstEII      BstPI       BstXI       DrdI        
Eam1105I    EcoO65I     EcoT22I     FseI        HpaI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        SalI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       2395  GGTACC               G^GTACC
AccIII       1057  TCCGGA               T^CCGGA
AclI         3066  AACGTT               AA^CGTT
AcyI          683  GGCGCC               G[GA]^CG[CT]C
AflIII       4863  ACATGT               A^C[GA][CT]GT
Aor51HI      1872  AGCGCT               AGC^GCT
Asp700I      4804  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2395  GGTACC               G^GTACC
AspI         3496  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          80  TGCGCA               TGC^GCA
BbeI          683  GGCGCC               GGCGC^C
BglII        2792  AGATCT               A^GATCT
BlpI         2140  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2140  GCTGAGC              GC^T[ACGT]AGC
BsaHI         683  GGCGCC               G[GA]^CG[CT]C
BsaI          216  GAGACC               GGTCTC(1/5)
BseAI        1057  TCCGGA               T^CCGGA
BsgI         1884  GTGCAG               GTGCAG(16/14)
BsiWI         715  CGTACG               C^GTACG
BspDI          50  ATCGAT               AT^CGAT
BspEI        1057  TCCGGA               T^CCGGA
BspLU11I     4863  ACATGT               A^CATGT
Bst1107I       33  GTATAC               GTA^TAC
BstZ17I        33  GTATAC               GTA^TAC
CelII        2140  GCTGAGC              GC^T[ACGT]AGC
ClaI           50  ATCGAT               AT^CGAT
CpoI         1059  CGGACCG              CG^G[AT]CCG
DraIII       1972  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2460  CGGCCG               C^GGCCG
EclXI        2460  CGGCCG               C^GGCCG
Eco47III     1872  AGCGCT               AGC^GCT
Eco52I       2460  CGGCCG               C^GGCCG
EcoRI        3892  GAATTC               G^AATTC
EcoRV        2884  GATATC               GAT^ATC
FspI           80  TGCGCA               TGC^GCA
Hin1I         683  GGCGCC               G[GA]^CG[CT]C
KasI          683  GGCGCC               G^GCGCC
KpnI         2395  GGTACC               GGTAC^C
MroI         1057  TCCGGA               T^CCGGA
NarI          683  GGCGCC               GG^CGCC
NdeI         1930  CATATG               CA^TATG
PflFI        3496  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     3066  AACGTT               AA^CGTT
RsrII        1059  CGGACCG              CG^G[AT]CCG
SbfI          872  CCTGCAGG             CCTGCA^GG
SexAI         417  ACCTGGT              A^CC[AT]GGT
SfoI          683  GGCGCC               GGC^GCC
SpeI         5043  ACTAGT               A^CTAGT
Sse8387I      872  CCTGCAGG             CCTGCA^GG
Tth111I      3496  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         4804  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AflII        1316  CTTAAG               C^TTAAG
             3979  CTTAAG              
AlwI          466  GGATC                GGATC(4/5)
             2738  GGATC               
AocI          366  CCTCAGG              CC^T[ACGT]AGG
             2209  CCTCAGG             
AvaI         2062  CCCGAG               C^[CT]CG[GA]G
             2650  CTCGGG              
AvrII        2566  CCTAGG               C^CTAGG
             3248  CCTAGG              
BbsI          525  GTCTTC               GAAGAC(2/6)
              755  GTCTTC              
BbvCI         254  CCTCAGC              CCTCAGC(-5/-2)
              348  CCTCAGC             
BcgI         5640  CGATGAAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              982  GCAACGTGATCG        
BciVI        2722  GGATAC               GTATCC(6/5)
              183  GTATCC              
BfrI         1316  CTTAAG               C^TTAAG
             3979  CTTAAG              
BlnI         2566  CCTAGG               C^CTAGG
             3248  CCTAGG              
BpuAI         525  GTCTTC               GAAGAC(2/6)
              755  GTCTTC              
BsmAI         216  GAGAC                GTCTC(1/5)
             5289  GAGAC               
BsoBI        2062  CCCGAG               C^[CT]CG[GA]G
             2650  CTCGGG              
BspMI        4971  ACCTGC               ACCTGC(4/8)
             1432  GCAGGT              
BsrFI         248  GCCGGC               [GA]^CCGG[CT]
              611  GCCGGC              
BssSI        2426  CTCGTG               CACGAG(-5/-1)
             2594  CTCGTG              
BstAPI         83  GCAAACCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              768  GCAGCCGCTGC         
BstYI        2738  GGATCT               [GA]^GATC[CT]
             2792  AGATCT              
Bsu36I        366  CCTCAGG              CC^T[ACGT]AGG
             2209  CCTCAGG             
Cfr10I        248  GCCGGC               [GA]^CCGG[CT]
              611  GCCGGC              
EarI          492  CTCTTC               CTCTTC(1/4)
             3204  GAAGAG              
Eco81I        366  CCTCAGG              CC^T[ACGT]AGG
             2209  CCTCAGG             
HgaI         1186  GACGC                GACGC(5/10)
              390  GCGTC               
HincII        477  GTCAAC               GT[CT]^[GA]AC
              516  GTCAAC              
HindII        477  GTCAAC               GT[CT]^[GA]AC
              516  GTCAAC              
Ksp632I       492  CTCTTC               CTCTTC(1/4)
             3204  GAAGAG              
KspI          376  CCGCGG               CCGC^GG
              709  CCGCGG              
MflI         2738  GGATCT               [GA]^GATC[CT]
             2792  AGATCT              
NaeI          248  GCCGGC               GCC^GGC
              611  GCCGGC              
NcoI         1289  CCATGG               C^CATGG
             1632  CCATGG              
NgoMIV        248  GCCGGC               G^CCGGC
              611  GCCGGC              
NheI         3739  GCTAGC               G^CTAGC
             3771  GCTAGC              
PflMI         856  CCAACCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1030  CCAAGTCCTGG         
PleI          174  GAGTC                GAGTC(4/5)
             2937  GAGTC               
PvuII         809  CAGCTG               CAG^CTG
             2941  CAGCTG              
SacI         3446  GAGCTC               GAGCT^C
             3517  GAGCTC              
SacII         376  CCGCGG               CCGC^GG
              709  CCGCGG              
SapI         3204  GAAGAGC              GCTCTTC(1/4)
              491  GCTCTTC             
ScaI         2167  AGTACT               AGT^ACT
             3294  AGTACT              
StuI         3733  AGGCCT               AGG^CCT
             3765  AGGCCT              
Van91I        856  CCAACCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1030  CCAAGTCCTGG         
XcmI         1030  CCAAGTCCTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4154  CCAGCCCAGGTGTGG     
XhoII        2738  GGATCT               [GA]^GATC[CT]
             2792  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwNI       ApoI        AseI        AsnI        
AspHI       AvaII       BanI        BanII       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BsaWI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspHI       BsrBI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PpuMI       PshBI       PstI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI