##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AflII AgeI AscI BamHI
BfrI BglII BsiWI BspDI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DrdI FseI HpaI
KspI MfeI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PvuI
RsrII SacII SapI SbfI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 3048 GGTACC G^GTACC
AccIII 4989 TCCGGA T^CCGGA
AclI 5848 AACGTT AA^CGTT
AcyI 744 GGCGCC G[GA]^CG[CT]C
AhdI 90 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2032 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2937 AGCGCT AGC^GCT
Asp718I 3048 GGTACC G^GTACC
AspEI 90 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 571 TGCGCA TGC^GCA
AvrII 4593 CCTAGG C^CTAGG
BbeI 744 GGCGCC GGCGC^C
BbrPI 1968 CACGTG CAC^GTG
BbvCI 2771 GCTGAGG CCTCAGC(-5/-2)
BciVI 3827 GGATAC GTATCC(6/5)
BclI 2456 TGATCA T^GATCA
BglI 1207 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4593 CCTAGG C^CTAGG
BsaAI 1968 CACGTG [CT]AC^GT[GA]
BsaHI 744 GGCGCC G[GA]^CG[CT]C
BseAI 4989 TCCGGA T^CCGGA
BsiEI 763 CGGCCG CG[GA][CT]^CG
BsmBI 1498 GAGACG CGTCTC(1/5)
BspEI 4989 TCCGGA T^CCGGA
BsrFI 5027 GCCGGT [GA]^CCGG[CT]
BstEII 4120 GGTAACC G^GT[ACGT]ACC
BstPI 4120 GGTAACC G^GT[ACGT]ACC
BstXI 5055 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2032 CCTGAGG CC^T[ACGT]AGG
Cfr10I 5027 GCCGGT [GA]^CCGG[CT]
DraIII 437 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 763 CGGCCG C^GGCCG
Eam1105I 90 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 763 CGGCCG C^GGCCG
Eco47III 2937 AGCGCT AGC^GCT
Eco52I 763 CGGCCG C^GGCCG
Eco81I 2032 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4120 GGTAACC G^GT[ACGT]ACC
EcoRI 1416 GAATTC G^AATTC
FbaI 2456 TGATCA T^GATCA
FspI 571 TGCGCA TGC^GCA
Hin1I 744 GGCGCC G[GA]^CG[CT]C
KasI 744 GGCGCC G^GCGCC
KpnI 3048 GGTACC GGTAC^C
MluI 866 ACGCGT A^CGCGT
MroI 4989 TCCGGA T^CCGGA
NarI 744 GGCGCC GG^CGCC
PflFI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1968 CACGTG CAC^GTG
PmeI 5774 GTTTAAAC GTTT^AAAC
PmlI 1968 CACGTG CAC^GTG
PshAI 948 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5848 AACGTT AA^CGTT
SalI 98 GTCGAC G^TCGAC
ScaI 1854 AGTACT AGT^ACT
SexAI 3460 ACCAGGT A^CC[AT]GGT
SfoI 744 GGCGCC GGC^GCC
Tth111I 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 98 GTCGAC GT^[AC][GT]AC
1703 GTAGAC
Asp700I 2091 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3890 GAAAGCATTC
BalI 113 TGGCCA TGG^CCA
278 TGGCCA
BsaBI 227 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3613 GATGCAAATC
BsaI 899 GGTCTC GGTCTC(1/5)
1773 GGTCTC
BsaWI 1510 ACCGGA [AT]^CCGG[AT]
4989 TCCGGA
BspHI 962 TCATGA T^CATGA
1270 TCATGA
BspLU11I 3750 ACATGT A^CATGT
5670 ACATGT
BsrDI 4716 CATTGC GCAATG(2/0)
5621 GCAATG
EarI 2195 CTCTTC CTCTTC(1/4)
4917 GAAGAG
EcoRV 1069 GATATC GAT^ATC
1957 GATATC
HgaI 677 GCGTC GACGC(5/10)
868 GCGTC
Ksp632I 2195 CTCTTC CTCTTC(1/4)
4917 GAAGAG
MamI 227 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3613 GATGCAAATC
MluNI 113 TGGCCA TGG^CCA
278 TGGCCA
MscI 113 TGGCCA TGG^CCA
278 TGGCCA
PpuMI 844 GGGACCT [GA]G^G[AT]CC[CT]
1356 AGGACCC
PstI 2506 CTGCAG CTGCA^G
5243 CTGCAG
RcaI 962 TCATGA T^CATGA
1270 TCATGA
SacI 2697 GAGCTC GAGCT^C
4831 GAGCTC
SmaI 852 CCCGGG CCC^GGG
1308 CCCGGG
SspI 4615 AATATT AAT^ATT
5593 AATATT
XbaI 1055 TCTAGA T^CTAGA
1525 TCTAGA
XmaCI 852 CCCGGG C^CCGGG
1308 CCCGGG
XmaI 852 CCCGGG C^CCGGG
1308 CCCGGG
XmnI 2091 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3890 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AseI AsnI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII