##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccI AclI AflII AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbsI
BcgI BciVI BclI BfrI BlnI
BpuAI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FspI HaeII HincII HindII
HindIII HpaI KasI MluI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2081 GACGTC GACGT^C
AccII 2962 CGCG CG^CG
AccIII 4367 TCCGGA T^CCGGA
AflIII 4081 ACGTGT A^C[GA][CT]GT
AgeI 1129 ACCGGT A^CCGGT
BalI 4489 TGGCCA TGG^CCA
BbrPI 897 CACGTG CAC^GTG
BglII 3875 AGATCT A^GATCT
BsaAI 897 CACGTG [CT]AC^GT[GA]
BsaBI 1304 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4367 TCCGGA T^CCGGA
BsiEI 1842 CGGCCG CG[GA][CT]^CG
BsiWI 4248 CGTACG C^GTACG
BspEI 4367 TCCGGA T^CCGGA
BsrBI 3 CCGCTC CCGCTC(-3/-3)
BstAPI 3159 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2962 CGCG CG^CG
EagI 1842 CGGCCG C^GGCCG
EclXI 1842 CGGCCG C^GGCCG
Eco52I 1842 CGGCCG C^GGCCG
FseI 2170 GGCCGGCC GGCCGG^CC
KspI 2961 CCGCGG CCGC^GG
MamI 1304 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4489 TGGCCA TGG^CCA
MroI 4367 TCCGGA T^CCGGA
MscI 4489 TGGCCA TGG^CCA
MvnI 2962 CGCG CG^CG
PflMI 4018 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1129 ACCGGT A^CCGGT
PmaCI 897 CACGTG CAC^GTG
PmlI 897 CACGTG CAC^GTG
SacII 2961 CCGCGG CCGC^GG
SapI 1183 GCTCTTC GCTCTTC(1/4)
SbfI 3156 CCTGCAGG CCTGCA^GG
ScaI 446 AGTACT AGT^ACT
Sse8387I 3156 CCTGCAGG CCTGCA^GG
SspI 4872 AATATT AAT^ATT
Van91I 4018 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Alw44I 894 GTGCAC G^TGCAC
1534 GTGCAC
ApaLI 894 GTGCAC G^TGCAC
1534 GTGCAC
BbvCI 3897 CCTCAGC CCTCAGC(-5/-2)
2905 GCTGAGG
BsgI 4147 CTGCAC GTGCAG(16/14)
2351 GTGCAG
BsmBI 4831 CGTCTC CGTCTC(1/5)
3272 GAGACG
BsmI 240 GAATGC GAATGC(1/-1)
1991 GAATGC
BssSI 760 CACGAG CACGAG(-5/-1)
4485 CTCGTG
CfoI 731 GCGC GCG^C
3092 GCGC
DraI 3880 TTTAAA TTT^AAA
4934 TTTAAA
EcoNI 4502 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4576 CCTGCCCAAGG
HhaI 731 GCGC GCG^C
3092 GCGC
HinP1I 731 GCGC G^CGC
3092 GCGC
MslI 4200 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4398 CATCAGTGTG
PstI 3157 CTGCAG CTGCA^G
3960 CTGCAG
SfcI 3157 CTGCAG C^T[GA][CT]AG
3960 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MspA1I MspI MunI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PaeR7I PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfiI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoI XhoII XmaCI XmaI