##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BciVI BfrI BglII BlnI BseAI
BsiWI BspDI BspEI BspHI BsrDI
ClaI CpoI DraI EcoRV EcoT22I
FseI HindIII KpnI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmeI PshAI
PshBI Psp1406I RcaI RsrII SbfI
ScaI SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AhdI 840 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 289 AGCGCT AGC^GCT
AspEI 840 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3991 TGCGCA TGC^GCA
BamHI 3787 GGATCC G^GATCC
BbrPI 1250 CACGTG CAC^GTG
BclI 5762 TGATCA T^GATCA
BsaWI 1035 TCCGGT [AT]^CCGG[AT]
BsmBI 451 CGTCTC CGTCTC(1/5)
BsmI 2823 GCATTC GAATGC(1/-1)
Bst1107I 757 GTATAC GTA^TAC
BstBI 1745 TTCGAA TT^CGAA
BstZ17I 757 GTATAC GTA^TAC
Eam1105I 840 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 289 AGCGCT AGC^GCT
FbaI 5762 TGATCA T^GATCA
FspI 3991 TGCGCA TGC^GCA
HpaI 5735 GTTAAC GTT^AAC
KspI 310 CCGCGG CCGC^GG
MluI 636 ACGCGT A^CGCGT
NspV 1745 TTCGAA TT^CGAA
PaeR7I 5268 CTCGAG C^TCGAG
PmaCI 1250 CACGTG CAC^GTG
PmlI 1250 CACGTG CAC^GTG
PvuI 5113 CGATCG CGAT^CG
SacI 2929 GAGCTC GAGCT^C
SacII 310 CCGCGG CCGC^GG
SapI 4826 GCTCTTC GCTCTTC(1/4)
SfiI 275 GGCCTCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1745 TTCGAA TT^CGAA
SgrAI 2003 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1207 ACTAGT A^CTAGT
SphI 4688 GCATGC GCATG^C
SspI 1475 AATATT AAT^ATT
XhoI 5268 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 3064 GTGCAC G^TGCAC
4673 GTGCAC
ApaLI 3064 GTGCAC G^TGCAC
4673 GTGCAC
AspI 4092 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5418 GACAGGGTC
BcgI 1456 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5121 CGATGCGGCTGC
BfaI 1208 CTAG C^TAG
3534 CTAG
BsaBI 2987 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4635 GATCAGCATC
Bsp1407I 411 TGTACA T^GTACA
2508 TGTACA
BspLU11I 1427 ACATGT A^CATGT
5809 ACATGT
BsrGI 411 TGTACA T^GTACA
2508 TGTACA
BssHII 67 GCGCGC G^CGCGC
5039 GCGCGC
BssSI 830 CACGAG CACGAG(-5/-1)
1051 CACGAG
BstAPI 424 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5273 GCAGCGGCTGC
DrdI 2738 GACAGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5175 GACGGGGTGGTC
EcoRI 3096 GAATTC G^AATTC
3252 GAATTC
MaeI 1208 CTAG C^TAG
3534 CTAG
MamI 2987 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4635 GATCAGCATC
PflFI 4092 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5418 GACAGGGTC
SalI 1610 GTCGAC G^TCGAC
1802 GTCGAC
SspBI 411 TGTACA T^GTACA
2508 TGTACA
Tth111I 4092 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5418 GACAGGGTC
XcmI 3034 CCACCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3137 CCAGGTTGGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI XmnI