##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AvrII       
BamHI       BcgI        BclI        BfrI        BlnI        
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
BspEI       BsrFI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FbaI        FseI        HindIII     HpaI        KpnI        
KspI        MamI        MluI        MroI        NaeI        
NdeI        NgoMIV      NotI        NruI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        ScaI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         2740  GTAGAC               GT^[AC][GT]AC
AccII        2651  CGCG                 CG^CG
AcyI         3025  GGCGCC               G[GA]^CG[CT]C
ApaI         1697  GGGCCC               GGGCC^C
AseI          643  ATTAAT               AT^TAAT
AsnI          643  ATTAAT               AT^TAAT
AviII        1760  TGCGCA               TGC^GCA
BbeI         3025  GGCGCC               GGCGC^C
BbrPI        1288  CACGTG               CAC^GTG
BbsI         2188  GAAGAC               GAAGAC(2/6)
BpuAI        2188  GAAGAC               GAAGAC(2/6)
BsaAI        1288  CACGTG               [CT]AC^GT[GA]
BsaHI        3025  GGCGCC               G[GA]^CG[CT]C
BspDI        2116  ATCGAT               AT^CGAT
BspHI         536  TCATGA               T^CATGA
BsrBI        1816  GAGCGG               CCGCTC(-3/-3)
BsrDI        2766  CATTGC               GCAATG(2/0)
BssSI         772  CTCGTG               CACGAG(-5/-1)
BstBI        1495  TTCGAA               TT^CGAA
BstUI        2651  CGCG                 CG^CG
ClaI         2116  ATCGAT               AT^CGAT
DraIII        610  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1513  GAATTC               G^AATTC
FspI         1760  TGCGCA               TGC^GCA
HaeII        3025  GGCGCC               [GA]GCGC^[CT]
Hin1I        3025  GGCGCC               G[GA]^CG[CT]C
HincII       1372  GTTGAC               GT[CT]^[GA]AC
HindII       1372  GTTGAC               GT[CT]^[GA]AC
KasI         3025  GGCGCC               G^GCGCC
MvnI         2651  CGCG                 CG^CG
NarI         3025  GGCGCC               GG^CGCC
NspV         1495  TTCGAA               TT^CGAA
PaeR7I       1180  CTCGAG               C^TCGAG
PmaCI        1288  CACGTG               CAC^GTG
PmlI         1288  CACGTG               CAC^GTG
PpuMI        2037  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         643  ATTAAT               AT^TAAT
RcaI          536  TCATGA               T^CATGA
SbfI          795  CCTGCAGG             CCTGCA^GG
SfiI         1641  GGCCCGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3025  GGCGCC               GGC^GCC
SfuI         1495  TTCGAA               TT^CGAA
Sse8387I      795  CCTGCAGG             CCTGCA^GG
XbaI         2856  TCTAGA               T^CTAGA
XcmI          404  CCAACACAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1180  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AspI         1612  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACCTTGTC           
BalI         1253  TGGCCA               TGG^CCA
             3173  TGGCCA              
BglI         1642  GCCCGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3028  GCCTACGAGGC         
BglII         732  AGATCT               A^GATCT
             1098  AGATCT              
BlpI         1582  GCTCAGC              GC^T[ACGT]AGC
             2920  GCTAAGC             
Bpu1102I     1582  GCTCAGC              GC^T[ACGT]AGC
             2920  GCTAAGC             
BsaWI         743  ACCGGA               [AT]^CCGG[AT]
             2934  ACCGGA              
BspLU11I      860  ACATGT               A^CATGT
             1211  ACATGT              
CelII        1582  GCTCAGC              GC^T[ACGT]AGC
             2920  GCTAAGC             
EcoNI         438  CCTTATCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2100  CCTTGTCAAGG         
EcoT22I       788  ATGCAT               ATGCA^T
              912  ATGCAT              
FauI          701  CCCGC                CCCGC(4/6)
             1818  GCGGG               
HgaI         1753  GACGC                GACGC(5/10)
             2718  GCGTC               
MfeI          680  CAATTG               C^AATTG
             1389  CAATTG              
MluNI        1253  TGGCCA               TGG^CCA
             3173  TGGCCA              
MscI         1253  TGGCCA               TGG^CCA
             3173  TGGCCA              
MunI          680  CAATTG               C^AATTG
             1389  CAATTG              
NcoI          155  CCATGG               C^CATGG
             2981  CCATGG              
NheI          321  GCTAGC               G^CTAGC
              399  GCTAGC              
NsiI          788  ATGCAT               ATGCA^T
              912  ATGCAT              
PflFI        1612  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACCTTGTC           
SexAI         611  ACCTGGT              A^CC[AT]GGT
             2570  ACCTGGT             
SmaI           35  CCCGGG               CCC^GGG
             1643  CCCGGG              
Tth111I      1612  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACCTTGTC           
XmaCI          35  CCCGGG               C^CCGGG
             1643  CCCGGG              
XmaI           35  CCCGGG               C^CCGGG
             1643  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BciVI       BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI