################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AvrII BamHI BcgI BclI BfrI BlnI BsaBI BseAI BsiEI BsiWI BsmBI BspEI BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FbaI FseI HindIII HpaI KpnI KspI MamI MluI MroI NaeI NdeI NgoMIV NotI NruI PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SapI ScaI SgrAI SnaBI SpeI SphI SspI SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2740 GTAGAC GT^[AC][GT]AC AccII 2651 CGCG CG^CG AcyI 3025 GGCGCC G[GA]^CG[CT]C ApaI 1697 GGGCCC GGGCC^C AseI 643 ATTAAT AT^TAAT AsnI 643 ATTAAT AT^TAAT AviII 1760 TGCGCA TGC^GCA BbeI 3025 GGCGCC GGCGC^C BbrPI 1288 CACGTG CAC^GTG BbsI 2188 GAAGAC GAAGAC(2/6) BpuAI 2188 GAAGAC GAAGAC(2/6) BsaAI 1288 CACGTG [CT]AC^GT[GA] BsaHI 3025 GGCGCC G[GA]^CG[CT]C BspDI 2116 ATCGAT AT^CGAT BspHI 536 TCATGA T^CATGA BsrBI 1816 GAGCGG CCGCTC(-3/-3) BsrDI 2766 CATTGC GCAATG(2/0) BssSI 772 CTCGTG CACGAG(-5/-1) BstBI 1495 TTCGAA TT^CGAA BstUI 2651 CGCG CG^CG ClaI 2116 ATCGAT AT^CGAT DraIII 610 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 1513 GAATTC G^AATTC FspI 1760 TGCGCA TGC^GCA HaeII 3025 GGCGCC [GA]GCGC^[CT] Hin1I 3025 GGCGCC G[GA]^CG[CT]C HincII 1372 GTTGAC GT[CT]^[GA]AC HindII 1372 GTTGAC GT[CT]^[GA]AC KasI 3025 GGCGCC G^GCGCC MvnI 2651 CGCG CG^CG NarI 3025 GGCGCC GG^CGCC NspV 1495 TTCGAA TT^CGAA PaeR7I 1180 CTCGAG C^TCGAG PmaCI 1288 CACGTG CAC^GTG PmlI 1288 CACGTG CAC^GTG PpuMI 2037 AGGACCC [GA]G^G[AT]CC[CT] PshBI 643 ATTAAT AT^TAAT RcaI 536 TCATGA T^CATGA SbfI 795 CCTGCAGG CCTGCA^GG SfiI 1641 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3025 GGCGCC GGC^GCC SfuI 1495 TTCGAA TT^CGAA Sse8387I 795 CCTGCAGG CCTGCA^GG XbaI 2856 TCTAGA T^CTAGA XcmI 404 CCAACACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1180 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AspI 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2098 GACCTTGTC BalI 1253 TGGCCA TGG^CCA 3173 TGGCCA BglI 1642 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3028 GCCTACGAGGC BglII 732 AGATCT A^GATCT 1098 AGATCT BlpI 1582 GCTCAGC GC^T[ACGT]AGC 2920 GCTAAGC Bpu1102I 1582 GCTCAGC GC^T[ACGT]AGC 2920 GCTAAGC BsaWI 743 ACCGGA [AT]^CCGG[AT] 2934 ACCGGA BspLU11I 860 ACATGT A^CATGT 1211 ACATGT CelII 1582 GCTCAGC GC^T[ACGT]AGC 2920 GCTAAGC EcoNI 438 CCTTATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2100 CCTTGTCAAGG EcoT22I 788 ATGCAT ATGCA^T 912 ATGCAT FauI 701 CCCGC CCCGC(4/6) 1818 GCGGG HgaI 1753 GACGC GACGC(5/10) 2718 GCGTC MfeI 680 CAATTG C^AATTG 1389 CAATTG MluNI 1253 TGGCCA TGG^CCA 3173 TGGCCA MscI 1253 TGGCCA TGG^CCA 3173 TGGCCA MunI 680 CAATTG C^AATTG 1389 CAATTG NcoI 155 CCATGG C^CATGG 2981 CCATGG NheI 321 GCTAGC G^CTAGC 399 GCTAGC NsiI 788 ATGCAT ATGCA^T 912 ATGCAT PflFI 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2098 GACCTTGTC SexAI 611 ACCTGGT A^CC[AT]GGT 2570 ACCTGGT SmaI 35 CCCGGG CCC^GGG 1643 CCCGGG Tth111I 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2098 GACCTTGTC XmaCI 35 CCCGGG C^CCGGG 1643 CCCGGG XmaI 35 CCCGGG C^CCGGG 1643 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI