##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
Alw44I      ApaLI       AseI        AsnI        AvrII       
BamHI       BbeI        BbvCI       BfrI        BglII       
BlnI        BsaI        BseAI       BsiWI       BsmBI       
BspEI       BssSI       Bst1107I    BstBI       BstZ17I     
CpoI        EcoRI       EcoRV       FseI        HincII      
HindII      HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         821  GACGTC               GACGT^C
Acc65I       3248  GGTACC               G^GTACC
AcsI         4112  AAATTT               [GA]^AATT[CT]
AhdI         1187  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         4112  AAATTT               [GA]^AATT[CT]
AscI          247  GGCGCGCC             GG^CGCGCC
Asp700I       994  GAAAGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3248  GGTACC               G^GTACC
AspEI        1187  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         474  TGCGCA               TGC^GCA
BbrPI        2970  CACGTG               CAC^GTG
BcgI         1207  CGAGCGCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         146  GGATAC               GTATCC(6/5)
BclI         1470  TGATCA               T^GATCA
BlpI          211  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      211  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2970  CACGTG               [CT]AC^GT[GA]
BsaBI        4161  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1412  ACCGGA               [AT]^CCGG[AT]
BsiEI         537  CGGCCG               CG[GA][CT]^CG
BspDI        1685  ATCGAT               AT^CGAT
BspLU11I     2543  ACATGT               A^CATGT
BsrFI         535  GCCGGC               [GA]^CCGG[CT]
BstEII       3168  GGTCACC              G^GT[ACGT]ACC
BstPI        3168  GGTCACC              G^GT[ACGT]ACC
CelII         211  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        535  GCCGGC               [GA]^CCGG[CT]
ClaI         1685  ATCGAT               AT^CGAT
DraI         4109  TTTAAA               TTT^AAA
DraIII       1804  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3463  GACAAACCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          537  CGGCCG               C^GGCCG
Eam1105I     1187  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         537  CGGCCG               C^GGCCG
Eco52I        537  CGGCCG               C^GGCCG
EcoO65I      3168  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2927  ATGCAT               ATGCA^T
FbaI         1470  TGATCA               T^GATCA
FspI          474  TGCGCA               TGC^GCA
HindIII      2571  AAGCTT               A^AGCTT
KpnI         3248  GGTACC               GGTAC^C
MamI         4161  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          535  GCCGGC               GCC^GGC
NgoMIV        535  GCCGGC               G^CCGGC
NruI          960  TCGCGA               TCG^CGA
NsiI         2927  ATGCAT               ATGCA^T
PmaCI        2970  CACGTG               CAC^GTG
PmlI         2970  CACGTG               CAC^GTG
SacI         2737  GAGCTC               GAGCT^C
SbfI          748  CCTGCAGG             CCTGCA^GG
ScaI          699  AGTACT               AGT^ACT
SexAI         687  ACCTGGT              A^CC[AT]GGT
Sse8387I      748  CCTGCAGG             CCTGCA^GG
SspI         4098  AATATT               AAT^ATT
XmnI          994  GAAAGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          341  GACGCC               G[GA]^CG[CT]C
              821  GACGTC              
AflIII       2543  ACATGT               A^C[GA][CT]GT
             2971  ACGTGT              
AspI          967  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1612  GACCGAGTC           
BfaI         2152  CTAG                 C^TAG
             4146  CTAG                
BsaHI         341  GACGCC               G[GA]^CG[CT]C
              821  GACGTC              
Bsp1407I      351  TGTACA               T^GTACA
             1123  TGTACA              
BspHI        4047  TCATGA               T^CATGA
             4061  TCATGA              
BsrBI        1153  CCGCTC               CCGCTC(-3/-3)
             1584  CCGCTC              
BsrGI         351  TGTACA               T^GTACA
             1123  TGTACA              
BssHII          9  GCGCGC               G^CGCGC
              248  GCGCGC              
EcoNI          51  CCTCAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              829  CCTGGTGGAGG         
Hin1I         341  GACGCC               G[GA]^CG[CT]C
              821  GACGTC              
MaeI         2152  CTAG                 C^TAG
             4146  CTAG                
PflFI         967  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1612  GACCGAGTC           
PflMI         190  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1359  CCACAGACTGG         
RcaI         4047  TCATGA               T^CATGA
             4061  TCATGA              
SfiI          538  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2394  GGCCTCCTGGGCC       
SphI         2827  GCATGC               GCATG^C
             4090  GCATGC              
SspBI         351  TGTACA               T^GTACA
             1123  TGTACA              
TfiI         2294  GAATC                G^A[AT]TC
             3570  GAATC               
Tth111I       967  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1612  GACCGAGTC           
Van91I        190  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1359  CCACAGACTGG         
XcmI          186  CCATCCAGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3490  CCAGCTGTGAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco47III    Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmaCI       XmaI