##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I ApaLI AseI AsnI AvrII
BamHI BbeI BbvCI BfrI BglII
BlnI BsaI BseAI BsiWI BsmBI
BspEI BssSI Bst1107I BstBI BstZ17I
CpoI EcoRI EcoRV FseI HincII
HindII HpaI KasI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SfoI SfuI SgrAI SnaBI SpeI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 821 GACGTC GACGT^C
Acc65I 3248 GGTACC G^GTACC
AcsI 4112 AAATTT [GA]^AATT[CT]
AhdI 1187 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4112 AAATTT [GA]^AATT[CT]
AscI 247 GGCGCGCC GG^CGCGCC
Asp700I 994 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3248 GGTACC G^GTACC
AspEI 1187 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 474 TGCGCA TGC^GCA
BbrPI 2970 CACGTG CAC^GTG
BcgI 1207 CGAGCGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 146 GGATAC GTATCC(6/5)
BclI 1470 TGATCA T^GATCA
BlpI 211 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 211 GCTGAGC GC^T[ACGT]AGC
BsaAI 2970 CACGTG [CT]AC^GT[GA]
BsaBI 4161 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1412 ACCGGA [AT]^CCGG[AT]
BsiEI 537 CGGCCG CG[GA][CT]^CG
BspDI 1685 ATCGAT AT^CGAT
BspLU11I 2543 ACATGT A^CATGT
BsrFI 535 GCCGGC [GA]^CCGG[CT]
BstEII 3168 GGTCACC G^GT[ACGT]ACC
BstPI 3168 GGTCACC G^GT[ACGT]ACC
CelII 211 GCTGAGC GC^T[ACGT]AGC
Cfr10I 535 GCCGGC [GA]^CCGG[CT]
ClaI 1685 ATCGAT AT^CGAT
DraI 4109 TTTAAA TTT^AAA
DraIII 1804 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3463 GACAAACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 537 CGGCCG C^GGCCG
Eam1105I 1187 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 537 CGGCCG C^GGCCG
Eco52I 537 CGGCCG C^GGCCG
EcoO65I 3168 GGTCACC G^GT[ACGT]ACC
EcoT22I 2927 ATGCAT ATGCA^T
FbaI 1470 TGATCA T^GATCA
FspI 474 TGCGCA TGC^GCA
HindIII 2571 AAGCTT A^AGCTT
KpnI 3248 GGTACC GGTAC^C
MamI 4161 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 535 GCCGGC GCC^GGC
NgoMIV 535 GCCGGC G^CCGGC
NruI 960 TCGCGA TCG^CGA
NsiI 2927 ATGCAT ATGCA^T
PmaCI 2970 CACGTG CAC^GTG
PmlI 2970 CACGTG CAC^GTG
SacI 2737 GAGCTC GAGCT^C
SbfI 748 CCTGCAGG CCTGCA^GG
ScaI 699 AGTACT AGT^ACT
SexAI 687 ACCTGGT A^CC[AT]GGT
Sse8387I 748 CCTGCAGG CCTGCA^GG
SspI 4098 AATATT AAT^ATT
XmnI 994 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 341 GACGCC G[GA]^CG[CT]C
821 GACGTC
AflIII 2543 ACATGT A^C[GA][CT]GT
2971 ACGTGT
AspI 967 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1612 GACCGAGTC
BfaI 2152 CTAG C^TAG
4146 CTAG
BsaHI 341 GACGCC G[GA]^CG[CT]C
821 GACGTC
Bsp1407I 351 TGTACA T^GTACA
1123 TGTACA
BspHI 4047 TCATGA T^CATGA
4061 TCATGA
BsrBI 1153 CCGCTC CCGCTC(-3/-3)
1584 CCGCTC
BsrGI 351 TGTACA T^GTACA
1123 TGTACA
BssHII 9 GCGCGC G^CGCGC
248 GCGCGC
EcoNI 51 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
829 CCTGGTGGAGG
Hin1I 341 GACGCC G[GA]^CG[CT]C
821 GACGTC
MaeI 2152 CTAG C^TAG
4146 CTAG
PflFI 967 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1612 GACCGAGTC
PflMI 190 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1359 CCACAGACTGG
RcaI 4047 TCATGA T^CATGA
4061 TCATGA
SfiI 538 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2394 GGCCTCCTGGGCC
SphI 2827 GCATGC GCATG^C
4090 GCATGC
SspBI 351 TGTACA T^GTACA
1123 TGTACA
TfiI 2294 GAATC G^A[AT]TC
3570 GAATC
Tth111I 967 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1612 GACCGAGTC
Van91I 190 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1359 CCACAGACTGG
XcmI 186 CCATCCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3490 CCAGCTGTGAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI