##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BbeI        
BbrPI       BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BseAI       
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
ClaI        DraI        DraIII      Eam1105I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NheI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SalI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspI        SwaI        Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI         1414  GTAGAC               GT^[AC][GT]AC
Aor51HI      2630  AGCGCT               AGC^GCT
AspI         2655  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1433  TGCGCA               TGC^GCA
BalI         2168  TGGCCA               TGG^CCA
BsaWI         976  TCCGGT               [AT]^CCGG[AT]
BsmI         2438  GCATTC               GAATGC(1/-1)
BsrDI        2291  GCAATG               GCAATG(2/0)
BssSI        1081  CACGAG               CACGAG(-5/-1)
BstEII        364  GGTGACC              G^GT[ACGT]ACC
BstPI         364  GGTGACC              G^GT[ACGT]ACC
DrdI          250  GACGTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2630  AGCGCT               AGC^GCT
EcoO65I       364  GGTGACC              G^GT[ACGT]ACC
FspI         1433  TGCGCA               TGC^GCA
MluNI        2168  TGGCCA               TGG^CCA
MscI         2168  TGGCCA               TGG^CCA
MslI         2658  CATGTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          327  GCCGGC               GCC^GGC
NcoI         2591  CCATGG               C^CATGG
NdeI          157  CATATG               CA^TATG
NgoMIV        327  GCCGGC               G^CCGGC
NotI         2793  GCGGCCGC             GC^GGCCGC
NruI         2490  TCGCGA               TCG^CGA
PaeR7I       2496  CTCGAG               C^TCGAG
PflFI        2655  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         1472  GCTCTTC              GCTCTTC(1/4)
SbfI          915  CCTGCAGG             CCTGCA^GG
SfiI         2169  GGCCAACGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      915  CCTGCAGG             CCTGCA^GG
Tsp509I      2451  AATT                 ^AATT
Tth111I      2655  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2496  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI         1497  GACGCC               G[GA]^CG[CT]C
             1626  GGCGTC              
AocI          466  CCTGAGG              CC^T[ACGT]AGG
             1687  CCTGAGG             
BamHI         118  GGATCC               G^GATCC
             1207  GGATCC              
BbvCI         739  GCTGAGG              CCTCAGC(-5/-2)
             1603  GCTGAGG             
BsaHI        1497  GACGCC               G[GA]^CG[CT]C
             1626  GGCGTC              
BsgI          922  GTGCAG               GTGCAG(16/14)
             1462  GTGCAG              
Bsp1407I      557  TGTACA               T^GTACA
             2339  TGTACA              
BsrFI         185  ACCGGC               [GA]^CCGG[CT]
              327  GCCGGC              
BsrGI         557  TGTACA               T^GTACA
             2339  TGTACA              
BstXI        1478  CCATCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1715  CCAGCCTGCTGG        
Bsu36I        466  CCTGAGG              CC^T[ACGT]AGG
             1687  CCTGAGG             
Cfr10I        185  ACCGGC               [GA]^CCGG[CT]
              327  GCCGGC              
CpoI         2575  CGGACCG              CG^G[AT]CCG
             2621  CGGACCG             
EarI         1473  CTCTTC               CTCTTC(1/4)
             2199  GAAGAG              
Eco81I        466  CCTGAGG              CC^T[ACGT]AGG
             1687  CCTGAGG             
Hin1I        1497  GACGCC               G[GA]^CG[CT]C
             1626  GGCGTC              
Ksp632I      1473  CTCTTC               CTCTTC(1/4)
             2199  GAAGAG              
KspI          928  CCGCGG               CCGC^GG
             2103  CCGCGG              
NspI          560  ACATGC               [GA]CATG^[CT]
             1151  ACATGC              
PflMI         891  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              982  CCAGTCCCTGG         
PvuII         721  CAGCTG               CAG^CTG
             1963  CAGCTG              
RsrII        2575  CGGACCG              CG^G[AT]CCG
             2621  CGGACCG             
SacII         928  CCGCGG               CCGC^GG
             2103  CCGCGG              
SmlI         1131  CTTGAG               C^T[CT][GA]AG
             2496  CTCGAG              
SspBI         557  TGTACA               T^GTACA
             2339  TGTACA              
StuI           20  AGGCCT               AGG^CCT
              176  AGGCCT              
Van91I        891  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              982  CCAGTCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI