################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I ClaI DraI DraIII Eam1105I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MseI MunI NarI NheI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 1414 GTAGAC GT^[AC][GT]AC Aor51HI 2630 AGCGCT AGC^GCT AspI 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1433 TGCGCA TGC^GCA BalI 2168 TGGCCA TGG^CCA BsaWI 976 TCCGGT [AT]^CCGG[AT] BsmI 2438 GCATTC GAATGC(1/-1) BsrDI 2291 GCAATG GCAATG(2/0) BssSI 1081 CACGAG CACGAG(-5/-1) BstEII 364 GGTGACC G^GT[ACGT]ACC BstPI 364 GGTGACC G^GT[ACGT]ACC DrdI 250 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2630 AGCGCT AGC^GCT EcoO65I 364 GGTGACC G^GT[ACGT]ACC FspI 1433 TGCGCA TGC^GCA MluNI 2168 TGGCCA TGG^CCA MscI 2168 TGGCCA TGG^CCA MslI 2658 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 327 GCCGGC GCC^GGC NcoI 2591 CCATGG C^CATGG NdeI 157 CATATG CA^TATG NgoMIV 327 GCCGGC G^CCGGC NotI 2793 GCGGCCGC GC^GGCCGC NruI 2490 TCGCGA TCG^CGA PaeR7I 2496 CTCGAG C^TCGAG PflFI 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 1472 GCTCTTC GCTCTTC(1/4) SbfI 915 CCTGCAGG CCTGCA^GG SfiI 2169 GGCCAACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 915 CCTGCAGG CCTGCA^GG Tsp509I 2451 AATT ^AATT Tth111I 2655 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2496 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1497 GACGCC G[GA]^CG[CT]C 1626 GGCGTC AocI 466 CCTGAGG CC^T[ACGT]AGG 1687 CCTGAGG BamHI 118 GGATCC G^GATCC 1207 GGATCC BbvCI 739 GCTGAGG CCTCAGC(-5/-2) 1603 GCTGAGG BsaHI 1497 GACGCC G[GA]^CG[CT]C 1626 GGCGTC BsgI 922 GTGCAG GTGCAG(16/14) 1462 GTGCAG Bsp1407I 557 TGTACA T^GTACA 2339 TGTACA BsrFI 185 ACCGGC [GA]^CCGG[CT] 327 GCCGGC BsrGI 557 TGTACA T^GTACA 2339 TGTACA BstXI 1478 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1715 CCAGCCTGCTGG Bsu36I 466 CCTGAGG CC^T[ACGT]AGG 1687 CCTGAGG Cfr10I 185 ACCGGC [GA]^CCGG[CT] 327 GCCGGC CpoI 2575 CGGACCG CG^G[AT]CCG 2621 CGGACCG EarI 1473 CTCTTC CTCTTC(1/4) 2199 GAAGAG Eco81I 466 CCTGAGG CC^T[ACGT]AGG 1687 CCTGAGG Hin1I 1497 GACGCC G[GA]^CG[CT]C 1626 GGCGTC Ksp632I 1473 CTCTTC CTCTTC(1/4) 2199 GAAGAG KspI 928 CCGCGG CCGC^GG 2103 CCGCGG NspI 560 ACATGC [GA]CATG^[CT] 1151 ACATGC PflMI 891 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 982 CCAGTCCCTGG PvuII 721 CAGCTG CAG^CTG 1963 CAGCTG RsrII 2575 CGGACCG CG^G[AT]CCG 2621 CGGACCG SacII 928 CCGCGG CCGC^GG 2103 CCGCGG SmlI 1131 CTTGAG C^T[CT][GA]AG 2496 CTCGAG SspBI 557 TGTACA T^GTACA 2339 TGTACA StuI 20 AGGCCT AGG^CCT 176 AGGCCT Van91I 891 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 982 CCAGTCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI