##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflIII AgeI AhdI Aor51HI ApoI
AscI AspEI AviII BbeI BbrPI
BglII BsaBI BsaHI BsiEI BsiWI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII Hin1I
HindIII HpaI KasI KspI MamI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 1121 GGTACC G^GTACC
AccII 173 CGCG CG^CG
AccIII 1845 TCCGGA T^CCGGA
AflII 1615 CTTAAG C^TTAAG
Alw44I 313 GTGCAC G^TGCAC
ApaI 72 GGGCCC GGGCC^C
ApaLI 313 GTGCAC G^TGCAC
AseI 1754 ATTAAT AT^TAAT
AsnI 1754 ATTAAT AT^TAAT
Asp700I 2172 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1121 GGTACC G^GTACC
BalI 1062 TGGCCA TGG^CCA
BamHI 168 GGATCC G^GATCC
BcgI 1090 CGATGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1195 GTATCC GTATCC(6/5)
BclI 1394 TGATCA T^GATCA
BfrI 1615 CTTAAG C^TTAAG
BglI 1858 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1587 TACGTG [CT]AC^GT[GA]
BseAI 1845 TCCGGA T^CCGGA
BsmBI 2241 CGTCTC CGTCTC(1/5)
BsmI 1576 GCATTC GAATGC(1/-1)
Bsp1407I 210 TGTACA T^GTACA
BspDI 1088 ATCGAT AT^CGAT
BspEI 1845 TCCGGA T^CCGGA
BsrBI 57 CCGCTC CCGCTC(-3/-3)
BsrGI 210 TGTACA T^GTACA
BssSI 161 CACGAG CACGAG(-5/-1)
BstUI 173 CGCG CG^CG
CfoI 302 GCGC GCG^C
ClaI 1088 ATCGAT AT^CGAT
DraIII 1934 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1039 GACGGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 439 CCATGG C^C[GA][CT]GG
EaeI 1062 TGGCCA [CT]^GGCC[GA]
EcoT22I 709 ATGCAT ATGCA^T
FbaI 1394 TGATCA T^GATCA
HhaI 302 GCGC GCG^C
HinP1I 302 GCGC G^CGC
HincII 290 GTCAAC GT[CT]^[GA]AC
HindII 290 GTCAAC GT[CT]^[GA]AC
KpnI 1121 GGTACC GGTAC^C
MfeI 330 CAATTG C^AATTG
MluNI 1062 TGGCCA TGG^CCA
MroI 1845 TCCGGA T^CCGGA
MscI 1062 TGGCCA TGG^CCA
MunI 330 CAATTG C^AATTG
MvnI 173 CGCG CG^CG
NcoI 439 CCATGG C^CATGG
NsiI 709 ATGCAT ATGCA^T
PaeR7I 305 CTCGAG C^TCGAG
PshBI 1754 ATTAAT AT^TAAT
SacI 1728 GAGCTC GAGCT^C
SapI 1949 GCTCTTC GCTCTTC(1/4)
ScaI 684 AGTACT AGT^ACT
SphI 1113 GCATGC GCATG^C
SspBI 210 TGTACA T^GTACA
XbaI 1250 TCTAGA T^CTAGA
XhoI 305 CTCGAG C^TCGAG
XmnI 2172 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvrII 16 CCTAGG C^CTAGG
2335 CCTAGG
BbvCI 2489 CCTCAGC CCTCAGC(-5/-2)
1461 GCTGAGG
BlnI 16 CCTAGG C^CTAGG
2335 CCTAGG
BsaWI 1845 TCCGGA [AT]^CCGG[AT]
2245 TCCGGT
BspMI 2072 ACCTGC ACCTGC(4/8)
1885 GCAGGT
BsrDI 1215 CATTGC GCAATG(2/0)
375 GCAATG
BstAPI 1933 GCACTAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2137 GCAGCCTTTGC
BstXI 1582 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1986 CCAGATAATTGG
BstYI 168 GGATCC [GA]^GATC[CT]
873 GGATCT
HgaI 1882 GACGC GACGC(5/10)
2090 GACGC
MflI 168 GGATCC [GA]^GATC[CT]
873 GGATCT
NspI 768 GCATGT [GA]CATG^[CT]
1113 GCATGC
PflMI 1069 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGAGTTTGG
SmaI 69 CCCGGG CCC^GGG
1915 CCCGGG
StuI 2148 AGGCCT AGG^CCT
2338 AGGCCT
TfiI 1389 GAATC G^A[AT]TC
2381 GAATC
Van91I 1069 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGAGTTTGG
XcmI 1065 CCATCCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2156 CCACACTCTGTCTGG
XhoII 168 GGATCC [GA]^GATC[CT]
873 GGATCT
XmaCI 69 CCCGGG C^CCGGG
1915 CCCGGG
XmaI 69 CCCGGG C^CCGGG
1915 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I