##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AccIII      AclI        AflII       Aor51HI     
AscI        AseI        AsnI        BbsI        BclI        
BfrI        BlpI        Bpu1102I    BpuAI       BsaBI       
BseAI       BsiWI       BspDI       BspEI       BssHII      
Bst1107I    BstZ17I     CelII       ClaI        CpoI        
DrdI        Eco47III    EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KspI        MamI        
MfeI        MroI        MunI        NdeI        NotI        
NruI        NsiI        PacI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SbfI        ScaI        SfiI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       4313  GGTACC               G^GTACC
AgeI         4316  ACCGGT               A^CCGGT
Asp718I      4313  GGTACC               G^GTACC
BbrPI        4239  CACGTG               CAC^GTG
BcgI         3466  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         617  GTATCC               GTATCC(6/5)
BglII         210  AGATCT               A^GATCT
BsaAI        4239  CACGTG               [CT]AC^GT[GA]
BsaWI        4316  ACCGGT               [AT]^CCGG[AT]
BsiEI         975  CGGCCG               CG[GA][CT]^CG
BspLU11I     1845  ACATGT               A^CATGT
BsrDI        5205  CATTGC               GCAATG(2/0)
BstBI        4085  TTCGAA               TT^CGAA
DraI         2608  TTTAAA               TTT^AAA
EagI          975  CGGCCG               C^GGCCG
EclXI         975  CGGCCG               C^GGCCG
Eco52I        975  CGGCCG               C^GGCCG
EcoRI        5131  GAATTC               G^AATTC
KpnI         4313  GGTACC               GGTAC^C
MluI         1863  ACGCGT               A^CGCGT
NaeI          324  GCCGGC               GCC^GGC
NcoI         3760  CCATGG               C^CATGG
NgoMIV        324  GCCGGC               G^CCGGC
NheI         3861  GCTAGC               G^CTAGC
NspV         4085  TTCGAA               TT^CGAA
PaeR7I       2285  CTCGAG               C^TCGAG
PflMI        1726  CCACCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        4316  ACCGGT               A^CCGGT
PmaCI        4239  CACGTG               CAC^GTG
PmlI         4239  CACGTG               CAC^GTG
SalI          341  GTCGAC               G^TCGAC
SapI          808  GCTCTTC              GCTCTTC(1/4)
SfuI         4085  TTCGAA               TT^CGAA
Van91I       1726  CCACCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2127  TCTAGA               T^CTAGA
XhoI         2285  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII       1845  ACATGT               A^C[GA][CT]GT
             1863  ACGCGT              
Alw44I        242  GTGCAC               G^TGCAC
             1859  GTGCAC              
ApaLI         242  GTGCAC               G^TGCAC
             1859  GTGCAC              
Asp700I      1357  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4485  GAATGGCTTC          
AspI          935  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5567  GACCCTGTC           
AviII         741  TGCGCA               TGC^GCA
              783  TGCGCA              
AvrII        4074  CCTAGG               C^CTAGG
             5068  CCTAGG              
BamHI        4105  GGATCC               G^GATCC
             4644  GGATCC              
BlnI         4074  CCTAGG               C^CTAGG
             5068  CCTAGG              
BsmI         3346  GAATGC               GAATGC(1/-1)
             4443  GCATTC              
Bsp1407I      864  TGTACA               T^GTACA
             5413  TGTACA              
BsrGI         864  TGTACA               T^GTACA
             5413  TGTACA              
BstEII        115  GGTCACC              G^GT[ACGT]ACC
             1536  GGTCACC             
BstPI         115  GGTCACC              G^GT[ACGT]ACC
             1536  GGTCACC             
EcoO65I       115  GGTCACC              G^GT[ACGT]ACC
             1536  GGTCACC             
FspI          741  TGCGCA               TGC^GCA
              783  TGCGCA              
HincII        341  GTCGAC               GT[CT]^[GA]AC
             4666  GTTGAC              
HindII        341  GTCGAC               GT[CT]^[GA]AC
             4666  GTTGAC              
NspI         1845  ACATGT               [GA]CATG^[CT]
             1877  GCATGT              
PflFI         935  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5567  GACCCTGTC           
SspBI         864  TGTACA               T^GTACA
             5413  TGTACA              
StuI         1571  AGGCCT               AGG^CCT
             3748  AGGCCT              
Tth111I       935  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5567  GACCCTGTC           
XcmI         2922  CCAGGGAGGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5204  CCATTGCTTCACTGG     
XmnI         1357  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4485  GAATGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AhdI        AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        AspEI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
Eam1105I    EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmaCI       XmaI