################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccIII AclI AflII Aor51HI AscI AseI AsnI BbsI BclI BfrI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstZ17I CelII ClaI CpoI DrdI Eco47III EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MamI MfeI MroI MunI NdeI NotI NruI NsiI PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 4313 GGTACC G^GTACC AgeI 4316 ACCGGT A^CCGGT Asp718I 4313 GGTACC G^GTACC BbrPI 4239 CACGTG CAC^GTG BcgI 3466 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 617 GTATCC GTATCC(6/5) BglII 210 AGATCT A^GATCT BsaAI 4239 CACGTG [CT]AC^GT[GA] BsaWI 4316 ACCGGT [AT]^CCGG[AT] BsiEI 975 CGGCCG CG[GA][CT]^CG BspLU11I 1845 ACATGT A^CATGT BsrDI 5205 CATTGC GCAATG(2/0) BstBI 4085 TTCGAA TT^CGAA DraI 2608 TTTAAA TTT^AAA EagI 975 CGGCCG C^GGCCG EclXI 975 CGGCCG C^GGCCG Eco52I 975 CGGCCG C^GGCCG EcoRI 5131 GAATTC G^AATTC KpnI 4313 GGTACC GGTAC^C MluI 1863 ACGCGT A^CGCGT NaeI 324 GCCGGC GCC^GGC NcoI 3760 CCATGG C^CATGG NgoMIV 324 GCCGGC G^CCGGC NheI 3861 GCTAGC G^CTAGC NspV 4085 TTCGAA TT^CGAA PaeR7I 2285 CTCGAG C^TCGAG PflMI 1726 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 4316 ACCGGT A^CCGGT PmaCI 4239 CACGTG CAC^GTG PmlI 4239 CACGTG CAC^GTG SalI 341 GTCGAC G^TCGAC SapI 808 GCTCTTC GCTCTTC(1/4) SfuI 4085 TTCGAA TT^CGAA Van91I 1726 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2127 TCTAGA T^CTAGA XhoI 2285 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 1845 ACATGT A^C[GA][CT]GT 1863 ACGCGT Alw44I 242 GTGCAC G^TGCAC 1859 GTGCAC ApaLI 242 GTGCAC G^TGCAC 1859 GTGCAC Asp700I 1357 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4485 GAATGGCTTC AspI 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5567 GACCCTGTC AviII 741 TGCGCA TGC^GCA 783 TGCGCA AvrII 4074 CCTAGG C^CTAGG 5068 CCTAGG BamHI 4105 GGATCC G^GATCC 4644 GGATCC BlnI 4074 CCTAGG C^CTAGG 5068 CCTAGG BsmI 3346 GAATGC GAATGC(1/-1) 4443 GCATTC Bsp1407I 864 TGTACA T^GTACA 5413 TGTACA BsrGI 864 TGTACA T^GTACA 5413 TGTACA BstEII 115 GGTCACC G^GT[ACGT]ACC 1536 GGTCACC BstPI 115 GGTCACC G^GT[ACGT]ACC 1536 GGTCACC EcoO65I 115 GGTCACC G^GT[ACGT]ACC 1536 GGTCACC FspI 741 TGCGCA TGC^GCA 783 TGCGCA HincII 341 GTCGAC GT[CT]^[GA]AC 4666 GTTGAC HindII 341 GTCGAC GT[CT]^[GA]AC 4666 GTTGAC NspI 1845 ACATGT [GA]CATG^[CT] 1877 GCATGT PflFI 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5567 GACCCTGTC SspBI 864 TGTACA T^GTACA 5413 TGTACA StuI 1571 AGGCCT AGG^CCT 3748 AGGCCT Tth111I 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5567 GACCCTGTC XcmI 2922 CCAGGGAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5204 CCATTGCTTCACTGG XmnI 1357 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4485 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI