##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AhdI AocI ApaI AscI
Asp700I AspEI AviII AvrII BamHI
BbrPI BbvCI BlnI BsaI BseAI
BsiEI BsiWI BspDI BspEI BssHII
BssSI Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoT14I FseI FspI MluI
MroI NaeI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PvuI RsrII SacI
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI Sse8387I StyI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 5367 GACGTC GACGT^C
Acc65I 103 GGTACC G^GTACC
AclI 3708 AACGTT AA^CGTT
AgeI 15 ACCGGT A^CCGGT
Alw44I 2254 GTGCAC G^TGCAC
AlwNI 4100 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 72 AGCGCT AGC^GCT
ApaLI 2254 GTGCAC G^TGCAC
AseI 3717 ATTAAT AT^TAAT
AsnI 3717 ATTAAT AT^TAAT
Asp718I 103 GGTACC G^GTACC
AvaI 128 CTCGGG C^[CT]CG[GA]G
BbeI 96 GGCGCC GGCGC^C
BcgI 831 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1102 GGATAC GTATCC(6/5)
BlpI 3634 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3634 GCTAAGC GC^T[ACGT]AGC
BsaAI 925 CACGTA [CT]AC^GT[GA]
BsaBI 1360 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 15 ACCGGT [AT]^CCGG[AT]
BsmBI 4983 CGTCTC CGTCTC(1/5)
BsoBI 128 CTCGGG C^[CT]CG[GA]G
BspMI 153 ACCTGC ACCTGC(4/8)
BsrFI 15 ACCGGT [GA]^CCGG[CT]
Bst1107I 3763 GTATAC GTA^TAC
BstAPI 2097 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 904 TTCGAA TT^CGAA
BstEII 3878 GGTTACC G^GT[ACGT]ACC
BstPI 3878 GGTTACC G^GT[ACGT]ACC
BstZ17I 3763 GTATAC GTA^TAC
CelII 3634 GCTAAGC GC^T[ACGT]AGC
Cfr10I 15 ACCGGT [GA]^CCGG[CT]
Eco47III 72 AGCGCT AGC^GCT
EcoO65I 3878 GGTTACC G^GT[ACGT]ACC
HindIII 5002 AAGCTT A^AGCTT
KasI 96 GGCGCC G^GCGCC
KpnI 103 GGTACC GGTAC^C
MamI 1360 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2080 CAATTG C^AATTG
MunI 2080 CAATTG C^AATTG
NarI 96 GGCGCC GG^CGCC
NdeI 2495 CATATG CA^TATG
NspV 904 TTCGAA TT^CGAA
PflMI 1133 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 15 ACCGGT A^CCGGT
PshBI 3717 ATTAAT AT^TAAT
Psp1406I 3708 AACGTT AA^CGTT
ScaI 5019 AGTACT AGT^ACT
SfoI 96 GGCGCC GGC^GCC
SfuI 904 TTCGAA TT^CGAA
SpeI 1941 ACTAGT A^CTAGT
StuI 696 AGGCCT AGG^CCT
SwaI 2383 ATTTAAAT ATTT^AAAT
Van91I 1133 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 667 TCTAGA T^CTAGA
XcmI 1590 CCAGCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3494 GTCTAC GT^[AC][GT]AC
3763 GTATAC
AspI 4225 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACCTTGTC
BalI 1167 TGGCCA TGG^CCA
2395 TGGCCA
BanI 96 GGCGCC G^G[CT][GA]CC
103 GGTACC
BclI 2571 TGATCA T^GATCA
4471 TGATCA
BglI 1201 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2307 GCCTTTTGGGC
BsmI 2214 GAATGC GAATGC(1/-1)
801 GCATTC
Bsp1407I 1881 TGTACA T^GTACA
3001 TGTACA
BspHI 1031 TCATGA T^CATGA
1320 TCATGA
BsrBI 41 CCGCTC CCGCTC(-3/-3)
5068 CCGCTC
BsrGI 1881 TGTACA T^GTACA
3001 TGTACA
BstXI 752 CCAATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3793 CCAAAATTTTGG
DraII 3018 AGGTCCT [GA]G^G[ACGT]CC[CT]
4560 GGGACCT
EaeI 1167 TGGCCA [CT]^GGCC[GA]
2395 TGGCCA
EcoO109I 3018 AGGTCCT [GA]G^G[ACGT]CC[CT]
4560 GGGACCT
EcoRI 1013 GAATTC G^AATTC
3234 GAATTC
EcoRV 765 GATATC GAT^ATC
2176 GATATC
FbaI 2571 TGATCA T^GATCA
4471 TGATCA
HgaI 47 GACGC GACGC(5/10)
146 GACGC
HpaI 3361 GTTAAC GTT^AAC
5191 GTTAAC
KspI 51 CCGCGG CCGC^GG
114 CCGCGG
MluNI 1167 TGGCCA TGG^CCA
2395 TGGCCA
MscI 1167 TGGCCA TGG^CCA
2395 TGGCCA
PflFI 4225 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACCTTGTC
PpuMI 3018 AGGTCCT [GA]G^G[AT]CC[CT]
4560 GGGACCT
PshAI 3862 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4970 GACACTTGTC
PstI 2881 CTGCAG CTGCA^G
3857 CTGCAG
PvuII 7 CAGCTG CAG^CTG
1204 CAGCTG
RcaI 1031 TCATGA T^CATGA
1320 TCATGA
SacII 51 CCGCGG CCGC^GG
114 CCGCGG
SapI 319 GCTCTTC GCTCTTC(1/4)
4546 GCTCTTC
SspBI 1881 TGTACA T^GTACA
3001 TGTACA
SspI 2457 AATATT AAT^ATT
2904 AATATT
Tth111I 4225 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRII
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII