##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AflIII AgeI AscI AvrII BbrPI
BlnI BsaAI BsaBI BsiWI BspHI
BspLU11I Bst1107I BstZ17I CpoI EcoRV
EcoT22I HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII ScaI SexAI SfiI SgrAI
SnaBI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3523 GGTACC G^GTACC
AccIII 1045 TCCGGA T^CCGGA
AclI 2979 AACGTT AA^CGTT
Aor51HI 1282 AGCGCT AGC^GCT
AseI 4374 ATTAAT AT^TAAT
AsnI 4374 ATTAAT AT^TAAT
Asp718I 3523 GGTACC G^GTACC
AspI 5745 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4654 GGATCC G^GATCC
BbvCI 447 GCTGAGG CCTCAGC(-5/-2)
BclI 2963 TGATCA T^GATCA
BglII 5121 AGATCT A^GATCT
BseAI 1045 TCCGGA T^CCGGA
BspDI 5815 ATCGAT AT^CGAT
BspEI 1045 TCCGGA T^CCGGA
BsrDI 5868 GCAATG GCAATG(2/0)
BssHII 437 GCGCGC G^CGCGC
BstBI 355 TTCGAA TT^CGAA
BstEII 2352 GGTCACC G^GT[ACGT]ACC
BstPI 2352 GGTCACC G^GT[ACGT]ACC
ClaI 5815 ATCGAT AT^CGAT
DraIII 70 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3075 CGGCCG C^GGCCG
EclXI 3075 CGGCCG C^GGCCG
Eco47III 1282 AGCGCT AGC^GCT
Eco52I 3075 CGGCCG C^GGCCG
EcoO65I 2352 GGTCACC G^GT[ACGT]ACC
EcoRI 2895 GAATTC G^AATTC
FbaI 2963 TGATCA T^GATCA
FseI 1948 GGCCGGCC GGCCGG^CC
HincII 3663 GTCGAC GT[CT]^[GA]AC
HindII 3663 GTCGAC GT[CT]^[GA]AC
HindIII 5036 AAGCTT A^AGCTT
KpnI 3523 GGTACC GGTAC^C
KspI 2467 CCGCGG CCGC^GG
MroI 1045 TCCGGA T^CCGGA
NcoI 4774 CCATGG C^CATGG
NspV 355 TTCGAA TT^CGAA
PflFI 5745 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4374 ATTAAT AT^TAAT
Psp1406I 2979 AACGTT AA^CGTT
SacII 2467 CCGCGG CCGC^GG
SalI 3663 GTCGAC G^TCGAC
SapI 3162 GAAGAGC GCTCTTC(1/4)
SbfI 1181 CCTGCAGG CCTGCA^GG
SfuI 355 TTCGAA TT^CGAA
SphI 3013 GCATGC GCATG^C
Sse8387I 1181 CCTGCAGG CCTGCA^GG
Tth111I 5745 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5202 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3663 GTCGAC GT^[AC][GT]AC
5569 GTAGAC
AflII 417 CTTAAG C^TTAAG
5896 CTTAAG
AhdI 1905 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4971 GACCCGACGTC
Alw44I 2745 GTGCAC G^TGCAC
4362 GTGCAC
AocI 3210 CCTGAGG CC^T[ACGT]AGG
4151 CCTTAGG
ApaLI 2745 GTGCAC G^TGCAC
4362 GTGCAC
AspEI 1905 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4971 GACCCGACGTC
AviII 658 TGCGCA TGC^GCA
3655 TGCGCA
BalI 670 TGGCCA TGG^CCA
3028 TGGCCA
BbeI 2 GGCGCC GGCGC^C
3820 GGCGCC
BcgI 1343 CGAGAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
230 GCAGGAACTTCG
BciVI 5601 GGATAC GTATCC(6/5)
5926 GGATAC
BfrI 417 CTTAAG C^TTAAG
5896 CTTAAG
BsaWI 1045 TCCGGA [AT]^CCGG[AT]
2191 TCCGGT
BsiEI 2849 CGACCG CG[GA][CT]^CG
3075 CGGCCG
BsmBI 4135 CGTCTC CGTCTC(1/5)
327 GAGACG
BsmI 3935 GAATGC GAATGC(1/-1)
955 GCATTC
Bsu36I 3210 CCTGAGG CC^T[ACGT]AGG
4151 CCTTAGG
DrdI 1850 GACTTCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3989 GACCAATGGGTC
Eam1105I 1905 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4971 GACCCGACGTC
Eco81I 3210 CCTGAGG CC^T[ACGT]AGG
4151 CCTTAGG
FspI 658 TGCGCA TGC^GCA
3655 TGCGCA
HgaI 52 GCGTC GACGC(5/10)
3281 GCGTC
KasI 2 GGCGCC G^GCGCC
3820 GGCGCC
MluNI 670 TGGCCA TGG^CCA
3028 TGGCCA
MscI 670 TGGCCA TGG^CCA
3028 TGGCCA
NaeI 1949 GCCGGC GCC^GGC
4455 GCCGGC
NarI 2 GGCGCC GG^CGCC
3820 GGCGCC
NgoMIV 1949 GCCGGC G^CCGGC
4455 GCCGGC
SfoI 2 GGCGCC GGC^GCC
3820 GGCGCC
StuI 1762 AGGCCT AGG^CCT
2530 AGGCCT
XcmI 1873 CCATCGGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3024 CCAGTGGCCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI
XmnI