##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BlnI BsiEI BsiWI BspHI
BsrBI BsrFI BssHII Bst1107I BstUI
BstZ17I Cfr10I CpoI DraIII DrdI
EagI EclXI Eco52I EcoRV FseI
FspI KasI KpnI KspI MluI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SfiI SfoI SgrAI
SmaI SspI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 4194 TCCGGA T^CCGGA
AcyI 1632 GGCGTC G[GA]^CG[CT]C
AflII 1561 CTTAAG C^TTAAG
AhdI 3036 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2735 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1128 AGCGCT AGC^GCT
AspEI 3036 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1215 GTATCC GTATCC(6/5)
BclI 114 TGATCA T^GATCA
BcnI 5155 CCGGG CC^[GC]GG
BfrI 1561 CTTAAG C^TTAAG
BglI 2115 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1632 GGCGTC G[GA]^CG[CT]C
BsaWI 4194 TCCGGA [AT]^CCGG[AT]
BseAI 4194 TCCGGA T^CCGGA
BsmBI 4964 CGTCTC CGTCTC(1/5)
BsmI 1933 GAATGC GAATGC(1/-1)
BspDI 2453 ATCGAT AT^CGAT
BspEI 4194 TCCGGA T^CCGGA
BspLU11I 1678 ACATGT A^CATGT
BssSI 3393 CTCGTG CACGAG(-5/-1)
BstBI 3803 TTCGAA TT^CGAA
BstEII 3724 GGTGACC G^GT[ACGT]ACC
BstPI 3724 GGTGACC G^GT[ACGT]ACC
Bsu36I 2735 CCTGAGG CC^T[ACGT]AGG
ClaI 2453 ATCGAT AT^CGAT
DraI 4760 TTTAAA TTT^AAA
DraII 4208 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 3036 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1128 AGCGCT AGC^GCT
Eco81I 2735 CCTGAGG CC^T[ACGT]AGG
EcoO109I 4208 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3724 GGTGACC G^GT[ACGT]ACC
FbaI 114 TGATCA T^GATCA
HgaI 1633 GCGTC GACGC(5/10)
Hin1I 1632 GGCGTC G[GA]^CG[CT]C
HpaI 254 GTTAAC GTT^AAC
MroI 4194 TCCGGA T^CCGGA
NciI 5155 CCGGG CC^[GC]GG
NheI 2543 GCTAGC G^CTAGC
NspV 3803 TTCGAA TT^CGAA
PaeR7I 1187 CTCGAG C^TCGAG
PflMI 2059 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4208 AGGACCT [GA]G^G[AT]CC[CT]
SalI 991 GTCGAC G^TCGAC
SbfI 3229 CCTGCAGG CCTGCA^GG
SfuI 3803 TTCGAA TT^CGAA
SnaBI 888 TACGTA TAC^GTA
SpeI 4649 ACTAGT A^CTAGT
Sse8387I 3229 CCTGCAGG CCTGCA^GG
Van91I 2059 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3711 CCACCCGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1187 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspI 827 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5117 GACTCTGTC
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
5035 GCTGAGG
BcgI 403 GCAGGATAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1087 GCAGATGAGTCG
BglII 964 AGATCT A^GATCT
1866 AGATCT
BsaBI 3165 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4700 GATTTTCATC
BsgI 5085 CTGCAC GTGCAG(16/14)
2828 GTGCAG
BstAPI 241 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2743 GCAACAGATGC
DsaI 504 CCATGG C^C[GA][CT]GG
1249 CCATGG
EcoNI 1048 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1939 CCTAAACAAGG
FauI 480 CCCGC CCCGC(4/6)
5004 GCGGG
MamI 3165 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4700 GATTTTCATC
NcoI 504 CCATGG C^CATGG
1249 CCATGG
PflFI 827 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5117 GACTCTGTC
SexAI 4038 ACCTGGT A^CC[AT]GGT
5221 ACCTGGT
Tth111I 827 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5117 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PmaCI PmlI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI