################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AvrII BalI BamHI BanI BbeI BcgI BcnI BglII BlnI BsaBI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI DrdI DsaI EaeI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FseI Hin1I KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NciI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 96 CGCG CG^CG AccIII 1816 TCCGGA T^CCGGA AflII 2336 CTTAAG C^TTAAG AflIII 2923 ACACGT A^C[GA][CT]GT AhdI 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2661 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 447 AGCGCT AGC^GCT AseI 3207 ATTAAT AT^TAAT AsnI 3207 ATTAAT AT^TAAT AspEI 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 181 CTCGGG C^[CT]CG[GA]G AviII 1898 TGCGCA TGC^GCA BfrI 2336 CTTAAG C^TTAAG BlpI 1436 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1436 GCTCAGC GC^T[ACGT]AGC BseAI 1816 TCCGGA T^CCGGA BseRI 984 CTCCTC GAGGAG(10/8) BsoBI 181 CTCGGG C^[CT]CG[GA]G BspEI 1816 TCCGGA T^CCGGA BspHI 2833 TCATGA T^CATGA BspMI 835 GCAGGT ACCTGC(4/8) BsrBI 733 GAGCGG CCGCTC(-3/-3) BsrDI 468 GCAATG GCAATG(2/0) BstAPI 1556 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 96 CGCG CG^CG CelII 1436 GCTCAGC GC^T[ACGT]AGC DraIII 1116 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2443 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 447 AGCGCT AGC^GCT EcoNI 2518 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1662 CCAAGG C^C[AT][AT]GG EcoT22I 858 ATGCAT ATGCA^T FspI 1898 TGCGCA TGC^GCA HgaI 97 GCGTC GACGC(5/10) MroI 1816 TCCGGA T^CCGGA MslI 2924 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 96 CGCG CG^CG NheI 2005 GCTAGC G^CTAGC NsiI 858 ATGCAT ATGCA^T PshAI 517 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3207 ATTAAT AT^TAAT PstI 1554 CTGCAG CTGCA^G PvuII 1970 CAGCTG CAG^CTG RcaI 2833 TCATGA T^CATGA ScaI 1462 AGTACT AGT^ACT StyI 1662 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AspI 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2837 GACTCAGTC BbrPI 2061 CACGTG CAC^GTG 2924 CACGTG BciVI 485 GTATCC GTATCC(6/5) 960 GTATCC BclI 1746 TGATCA T^GATCA 2743 TGATCA BglI 74 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 226 GCCTGAATGGC BsaAI 2061 CACGTG [CT]AC^GT[GA] 2924 CACGTG BsaI 123 GAGACC GGTCTC(1/5) 2652 GAGACC Bst1107I 1603 GTATAC GTA^TAC 1688 GTATAC BstXI 964 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1969 CCAGCTGGATGG BstYI 719 GGATCT [GA]^GATC[CT] 2213 GGATCT BstZ17I 1603 GTATAC GTA^TAC 1688 GTATAC DraI 1174 TTTAAA TTT^AAA 2870 TTTAAA DraII 2327 GGGACCT [GA]G^G[ACGT]CC[CT] 2397 AGGACCT EcoO109I 2327 GGGACCT [GA]G^G[ACGT]CC[CT] 2397 AGGACCT FbaI 1746 TGATCA T^GATCA 2743 TGATCA HaeII 447 AGCGCT [GA]GCGC^[CT] 1421 AGCGCC HincII 2887 GTTAAC GT[CT]^[GA]AC 3129 GTTAAC HindII 2887 GTTAAC GT[CT]^[GA]AC 3129 GTTAAC HindIII 608 AAGCTT A^AGCTT 932 AAGCTT HpaI 2887 GTTAAC GTT^AAC 3129 GTTAAC MflI 719 GGATCT [GA]^GATC[CT] 2213 GGATCT MspA1I 536 CAGCGG C[AC]G^C[GT]G 1970 CAGCTG NdeI 861 CATATG CA^TATG 1750 CATATG NspI 1125 ACATGC [GA]CATG^[CT] 2141 ACATGC PflFI 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2837 GACTCAGTC PmaCI 2061 CACGTG CAC^GTG 2924 CACGTG PmlI 2061 CACGTG CAC^GTG 2924 CACGTG PpuMI 2327 GGGACCT [GA]G^G[AT]CC[CT] 2397 AGGACCT SacI 103 GAGCTC GAGCT^C 113 GAGCTC SapI 730 GAAGAGC GCTCTTC(1/4) 1037 GAAGAGC SpeI 1157 ACTAGT A^CTAGT 3170 ACTAGT Tth111I 904 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2837 GACTCAGTC XhoII 719 GGATCT [GA]^GATC[CT] 2213 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanII BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI