##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp718I     AvrII       BalI        BamHI       BanI        
BbeI        BcgI        BcnI        BglII       BlnI        
BsaBI       BsaHI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstEII      BstPI       
Bsu36I      Cfr10I      ClaI        CpoI        DrdI        
DsaI        EaeI        EagI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       FauI        
FseI        Hin1I       KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NciI        NcoI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII          96  CGCG                 CG^CG
AccIII       1816  TCCGGA               T^CCGGA
AflII        2336  CTTAAG               C^TTAAG
AflIII       2923  ACACGT               A^C[GA][CT]GT
AhdI         2443  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        2661  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       447  AGCGCT               AGC^GCT
AseI         3207  ATTAAT               AT^TAAT
AsnI         3207  ATTAAT               AT^TAAT
AspEI        2443  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          181  CTCGGG               C^[CT]CG[GA]G
AviII        1898  TGCGCA               TGC^GCA
BfrI         2336  CTTAAG               C^TTAAG
BlpI         1436  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1436  GCTCAGC              GC^T[ACGT]AGC
BseAI        1816  TCCGGA               T^CCGGA
BseRI         984  CTCCTC               GAGGAG(10/8)
BsoBI         181  CTCGGG               C^[CT]CG[GA]G
BspEI        1816  TCCGGA               T^CCGGA
BspHI        2833  TCATGA               T^CATGA
BspMI         835  GCAGGT               ACCTGC(4/8)
BsrBI         733  GAGCGG               CCGCTC(-3/-3)
BsrDI         468  GCAATG               GCAATG(2/0)
BstAPI       1556  GCAGATTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          96  CGCG                 CG^CG
CelII        1436  GCTCAGC              GC^T[ACGT]AGC
DraIII       1116  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2443  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      447  AGCGCT               AGC^GCT
EcoNI        2518  CCTTGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1662  CCAAGG               C^C[AT][AT]GG
EcoT22I       858  ATGCAT               ATGCA^T
FspI         1898  TGCGCA               TGC^GCA
HgaI           97  GCGTC                GACGC(5/10)
MroI         1816  TCCGGA               T^CCGGA
MslI         2924  CACGTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           96  CGCG                 CG^CG
NheI         2005  GCTAGC               G^CTAGC
NsiI          858  ATGCAT               ATGCA^T
PshAI         517  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3207  ATTAAT               AT^TAAT
PstI         1554  CTGCAG               CTGCA^G
PvuII        1970  CAGCTG               CAG^CTG
RcaI         2833  TCATGA               T^CATGA
ScaI         1462  AGTACT               AGT^ACT
StyI         1662  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AspI          904  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACTCAGTC           
BbrPI        2061  CACGTG               CAC^GTG
             2924  CACGTG              
BciVI         485  GTATCC               GTATCC(6/5)
              960  GTATCC              
BclI         1746  TGATCA               T^GATCA
             2743  TGATCA              
BglI           74  GCCTCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              226  GCCTGAATGGC         
BsaAI        2061  CACGTG               [CT]AC^GT[GA]
             2924  CACGTG              
BsaI          123  GAGACC               GGTCTC(1/5)
             2652  GAGACC              
Bst1107I     1603  GTATAC               GTA^TAC
             1688  GTATAC              
BstXI         964  CCAGCAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1969  CCAGCTGGATGG        
BstYI         719  GGATCT               [GA]^GATC[CT]
             2213  GGATCT              
BstZ17I      1603  GTATAC               GTA^TAC
             1688  GTATAC              
DraI         1174  TTTAAA               TTT^AAA
             2870  TTTAAA              
DraII        2327  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2397  AGGACCT             
EcoO109I     2327  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2397  AGGACCT             
FbaI         1746  TGATCA               T^GATCA
             2743  TGATCA              
HaeII         447  AGCGCT               [GA]GCGC^[CT]
             1421  AGCGCC              
HincII       2887  GTTAAC               GT[CT]^[GA]AC
             3129  GTTAAC              
HindII       2887  GTTAAC               GT[CT]^[GA]AC
             3129  GTTAAC              
HindIII       608  AAGCTT               A^AGCTT
              932  AAGCTT              
HpaI         2887  GTTAAC               GTT^AAC
             3129  GTTAAC              
MflI          719  GGATCT               [GA]^GATC[CT]
             2213  GGATCT              
MspA1I        536  CAGCGG               C[AC]G^C[GT]G
             1970  CAGCTG              
NdeI          861  CATATG               CA^TATG
             1750  CATATG              
NspI         1125  ACATGC               [GA]CATG^[CT]
             2141  ACATGC              
PflFI         904  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACTCAGTC           
PmaCI        2061  CACGTG               CAC^GTG
             2924  CACGTG              
PmlI         2061  CACGTG               CAC^GTG
             2924  CACGTG              
PpuMI        2327  GGGACCT              [GA]G^G[AT]CC[CT]
             2397  AGGACCT             
SacI          103  GAGCTC               GAGCT^C
              113  GAGCTC              
SapI          730  GAAGAGC              GCTCTTC(1/4)
             1037  GAAGAGC             
SpeI         1157  ACTAGT               A^CTAGT
             3170  ACTAGT              
Tth111I       904  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACTCAGTC           
XhoII         719  GGATCT               [GA]^GATC[CT]
             2213  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BanII       BbsI        BbvCI       BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XmnI