##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AvrII BbrPI BfrI BglII BlnI
BsaBI BsiEI BsiWI BspDI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 2561 TCCGGA T^CCGGA
AseI 506 ATTAAT AT^TAAT
AsnI 506 ATTAAT AT^TAAT
AviII 5126 TGCGCA TGC^GCA
BcgI 4400 GCAGTCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2694 GGATAC GTATCC(6/5)
BclI 1069 TGATCA T^GATCA
BglI 4467 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1231 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1231 GCTCAGC GC^T[ACGT]AGC
BsaAI 2920 CACGTA [CT]AC^GT[GA]
BseAI 2561 TCCGGA T^CCGGA
BsmBI 5509 GAGACG CGTCTC(1/5)
BspEI 2561 TCCGGA T^CCGGA
BspHI 240 TCATGA T^CATGA
BspLU11I 5586 ACATGT A^CATGT
BsrBI 2644 GAGCGG CCGCTC(-3/-3)
BssHII 47 GCGCGC G^CGCGC
BstAPI 4558 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 815 GGTGACC G^GT[ACGT]ACC
BstPI 815 GGTGACC G^GT[ACGT]ACC
CelII 1231 GCTCAGC GC^T[ACGT]AGC
DraIII 5712 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 973 GACAGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 569 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 815 GGTGACC G^GT[ACGT]ACC
EcoRI 547 GAATTC G^AATTC
FbaI 1069 TGATCA T^GATCA
FspI 5126 TGCGCA TGC^GCA
MroI 2561 TCCGGA T^CCGGA
PshAI 4456 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 506 ATTAAT AT^TAAT
RcaI 240 TCATGA T^CATGA
SalI 2327 GTCGAC G^TCGAC
SspI 282 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2327 GTCGAC GT^[AC][GT]AC
4260 GTAGAC
Alw44I 191 GTGCAC G^TGCAC
2763 GTGCAC
ApaLI 191 GTGCAC G^TGCAC
2763 GTGCAC
Asp700I 3502 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3607 GAATGTTTTC
AspI 299 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1214 GACGAGGTC
BamHI 2243 GGATCC G^GATCC
2954 GGATCC
BbeI 1270 GGCGCC GGCGC^C
5766 GGCGCC
BsaWI 978 TCCGGT [AT]^CCGG[AT]
2561 TCCGGA
BssSI 1856 CACGAG CACGAG(-5/-1)
4605 CTCGTG
DraI 2399 TTTAAA TTT^AAA
5181 TTTAAA
EcoRV 2105 GATATC GAT^ATC
5540 GATATC
KasI 1270 GGCGCC G^GCGCC
5766 GGCGCC
NarI 1270 GGCGCC GG^CGCC
5766 GGCGCC
NcoI 3677 CCATGG C^CATGG
4991 CCATGG
PaeR7I 1046 CTCGAG C^TCGAG
2214 CTCGAG
PflFI 299 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1214 GACGAGGTC
SfoI 1270 GGCGCC GGC^GCC
5766 GGCGCC
StuI 2122 AGGCCT AGG^CCT
5111 AGGCCT
Tth111I 299 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1214 GACGAGGTC
XhoI 1046 CTCGAG C^TCGAG
2214 CTCGAG
XmnI 3502 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3607 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmaCI XmaI