##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
Aor51HI     ApaI        AscI        Asp718I     AviII       
BbeI        BbrPI       BclI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BsrBI       BssHII      BstAPI      BstBI       BstEII      
BstPI       ClaI        CpoI        DrdI        Eco47III    
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HaeII       HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI          609  GACGCC               G[GA]^CG[CT]C
AhdI         1987  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1975  GTGCAC               G^TGCAC
ApaLI        1975  GTGCAC               G^TGCAC
AseI         5712  ATTAAT               AT^TAAT
AsnI         5712  ATTAAT               AT^TAAT
AspEI        1987  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1857  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        1188  GGATAC               GTATCC(6/5)
BglII        5179  AGATCT               A^GATCT
BlpI         3569  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3569  GCTGAGC              GC^T[ACGT]AGC
BsaHI         609  GACGCC               G[GA]^CG[CT]C
BsiEI         107  CGGCCG               CG[GA][CT]^CG
BspHI        1732  TCATGA               T^CATGA
BspMI        2824  ACCTGC               ACCTGC(4/8)
BssSI        4719  CACGAG               CACGAG(-5/-1)
CelII        3569  GCTGAGC              GC^T[ACGT]AGC
DraIII       5521  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          107  CGGCCG               C^GGCCG
Eam1105I     1987  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         107  CGGCCG               C^GGCCG
Eco52I        107  CGGCCG               C^GGCCG
EcoRI        2106  GAATTC               G^AATTC
EcoRV         726  GATATC               GAT^ATC
Hin1I         609  GACGCC               G[GA]^CG[CT]C
HindIII       541  AAGCTT               A^AGCTT
MaeII        5469  ACGT                 A^CGT
MfeI         1539  CAATTG               C^AATTG
MunI         1539  CAATTG               C^AATTG
NaeI          105  GCCGGC               GCC^GGC
NgoMIV        105  GCCGGC               G^CCGGC
PaeR7I        117  CTCGAG               C^TCGAG
PflFI        1857  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2469  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1143  GACGCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5712  ATTAAT               AT^TAAT
RcaI         1732  TCATGA               T^CATGA
SalI         1140  GTCGAC               G^TCGAC
SapI         1018  GAAGAGC              GCTCTTC(1/4)
ScaI         3699  AGTACT               AGT^ACT
SphI         4433  GCATGC               GCATG^C
Tth111I      1857  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2469  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          117  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII        505  ACATGT               A^C[GA][CT]GT
             5164  ACATGT              
Asp700I      3291  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5209  GAAGTTTTTC          
BamHI        1560  GGATCC               G^GATCC
             5385  GGATCC              
BcgI         2487  CGATTCTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2776  CGACCAAGCTGC        
BsmI         3807  GAATGC               GAATGC(1/-1)
             4694  GCATTC              
Bsp1407I      646  TGTACA               T^GTACA
             1846  TGTACA              
BspLU11I      505  ACATGT               A^CATGT
             5164  ACATGT              
BsrFI         105  GCCGGC               [GA]^CCGG[CT]
             2524  ACCGGC              
BsrGI         646  TGTACA               T^GTACA
             1846  TGTACA              
Bst1107I      261  GTATAC               GTA^TAC
             2342  GTATAC              
BstZ17I       261  GTATAC               GTA^TAC
             2342  GTATAC              
Cfr10I        105  GCCGGC               [GA]^CCGG[CT]
             2524  ACCGGC              
DraI          408  TTTAAA               TTT^AAA
             4570  TTTAAA              
DsaI         2955  CCATGG               C^C[GA][CT]GG
             3005  CCATGG              
HincII       1140  GTCGAC               GT[CT]^[GA]AC
             1797  GTTGAC              
HindII       1140  GTCGAC               GT[CT]^[GA]AC
             1797  GTTGAC              
NcoI         2955  CCATGG               C^CATGG
             3005  CCATGG              
PpuMI         973  GGGTCCC              [GA]G^G[AT]CC[CT]
             4282  GGGACCT             
PstI         2799  CTGCAG               CTGCA^G
             2871  CTGCAG              
SspBI         646  TGTACA               T^GTACA
             1846  TGTACA              
SspI         3761  AATATT               AAT^ATT
             4529  AATATT              
StuI          229  AGGCCT               AGG^CCT
             5410  AGGCCT              
XmnI         3291  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5209  GAAGTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       AvrII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BfrI        BglI        
BlnI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII