################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Aor51HI ApaI AscI Asp718I AviII BbeI BbrPI BclI BsaAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII BstAPI BstBI BstEII BstPI ClaI CpoI DrdI Eco47III EcoO65I EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 609 GACGCC G[GA]^CG[CT]C AhdI 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1975 GTGCAC G^TGCAC ApaLI 1975 GTGCAC G^TGCAC AseI 5712 ATTAAT AT^TAAT AsnI 5712 ATTAAT AT^TAAT AspEI 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 1188 GGATAC GTATCC(6/5) BglII 5179 AGATCT A^GATCT BlpI 3569 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3569 GCTGAGC GC^T[ACGT]AGC BsaHI 609 GACGCC G[GA]^CG[CT]C BsiEI 107 CGGCCG CG[GA][CT]^CG BspHI 1732 TCATGA T^CATGA BspMI 2824 ACCTGC ACCTGC(4/8) BssSI 4719 CACGAG CACGAG(-5/-1) CelII 3569 GCTGAGC GC^T[ACGT]AGC DraIII 5521 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 107 CGGCCG C^GGCCG Eam1105I 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 107 CGGCCG C^GGCCG Eco52I 107 CGGCCG C^GGCCG EcoRI 2106 GAATTC G^AATTC EcoRV 726 GATATC GAT^ATC Hin1I 609 GACGCC G[GA]^CG[CT]C HindIII 541 AAGCTT A^AGCTT MaeII 5469 ACGT A^CGT MfeI 1539 CAATTG C^AATTG MunI 1539 CAATTG C^AATTG NaeI 105 GCCGGC GCC^GGC NgoMIV 105 GCCGGC G^CCGGC PaeR7I 117 CTCGAG C^TCGAG PflFI 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2469 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1143 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5712 ATTAAT AT^TAAT RcaI 1732 TCATGA T^CATGA SalI 1140 GTCGAC G^TCGAC SapI 1018 GAAGAGC GCTCTTC(1/4) ScaI 3699 AGTACT AGT^ACT SphI 4433 GCATGC GCATG^C Tth111I 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2469 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 117 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 505 ACATGT A^C[GA][CT]GT 5164 ACATGT Asp700I 3291 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5209 GAAGTTTTTC BamHI 1560 GGATCC G^GATCC 5385 GGATCC BcgI 2487 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2776 CGACCAAGCTGC BsmI 3807 GAATGC GAATGC(1/-1) 4694 GCATTC Bsp1407I 646 TGTACA T^GTACA 1846 TGTACA BspLU11I 505 ACATGT A^CATGT 5164 ACATGT BsrFI 105 GCCGGC [GA]^CCGG[CT] 2524 ACCGGC BsrGI 646 TGTACA T^GTACA 1846 TGTACA Bst1107I 261 GTATAC GTA^TAC 2342 GTATAC BstZ17I 261 GTATAC GTA^TAC 2342 GTATAC Cfr10I 105 GCCGGC [GA]^CCGG[CT] 2524 ACCGGC DraI 408 TTTAAA TTT^AAA 4570 TTTAAA DsaI 2955 CCATGG C^C[GA][CT]GG 3005 CCATGG HincII 1140 GTCGAC GT[CT]^[GA]AC 1797 GTTGAC HindII 1140 GTCGAC GT[CT]^[GA]AC 1797 GTTGAC NcoI 2955 CCATGG C^CATGG 3005 CCATGG PpuMI 973 GGGTCCC [GA]G^G[AT]CC[CT] 4282 GGGACCT PstI 2799 CTGCAG CTGCA^G 2871 CTGCAG SspBI 646 TGTACA T^GTACA 1846 TGTACA SspI 3761 AATATT AAT^ATT 4529 AATATT StuI 229 AGGCCT AGG^CCT 5410 AGGCCT XmnI 3291 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5209 GAAGTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BglI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII