##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I AspEI AspI BbrPI
BfrI BsaAI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I HpaI MamI MluI MroI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 655 GGTACC G^GTACC
AccI 284 GTAGAC GT^[AC][GT]AC
AcsI 2807 GAATTT [GA]^AATT[CT]
AgeI 714 ACCGGT A^CCGGT
ApoI 2807 GAATTT [GA]^AATT[CT]
Asp718I 655 GGTACC G^GTACC
AviII 1776 TGCGCA TGC^GCA
BalI 1425 TGGCCA TGG^CCA
BamHI 842 GGATCC G^GATCC
BbeI 89 GGCGCC GGCGC^C
BcgI 1290 CGACAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2127 GGATAC GTATCC(6/5)
BclI 579 TGATCA T^GATCA
BglII 496 AGATCT A^GATCT
BssSI 1998 CACGAG CACGAG(-5/-1)
BstAPI 2160 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2719 TTTAAA TTT^AAA
DrdI 358 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 579 TGATCA T^GATCA
FseI 2530 GGCCGGCC GGCCGG^CC
FspI 1776 TGCGCA TGC^GCA
HgaI 125 GCGTC GACGC(5/10)
HindIII 521 AAGCTT A^AGCTT
KasI 89 GGCGCC G^GCGCC
KpnI 655 GGTACC GGTAC^C
KspI 24 CCGCGG CCGC^GG
MaeII 399 ACGT A^CGT
MfeI 567 CAATTG C^AATTG
MluNI 1425 TGGCCA TGG^CCA
MscI 1425 TGGCCA TGG^CCA
MunI 567 CAATTG C^AATTG
NarI 89 GGCGCC GG^CGCC
NspI 2224 GCATGT [GA]CATG^[CT]
PflMI 2053 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 714 ACCGGT A^CCGGT
PshAI 987 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 199 CTGCAG CTGCA^G
SacII 24 CCGCGG CCGC^GG
SapI 2298 GCTCTTC GCTCTTC(1/4)
SfoI 89 GGCGCC GGC^GCC
SmaI 2536 CCCGGG CCC^GGG
SmlI 1751 CTCAAG C^T[CT][GA]AG
TfiI 1393 GATTC G^A[AT]TC
Van91I 2053 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2536 CCCGGG C^CCGGG
XmaI 2536 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 25 CGCG CG^CG
832 CGCG
AcyI 89 GGCGCC G[GA]^CG[CT]C
124 GGCGTC
AocI 625 CCTCAGG CC^T[ACGT]AGG
2462 CCTCAGG
Aor51HI 181 AGCGCT AGC^GCT
1920 AGCGCT
ApaI 883 GGGCCC GGGCC^C
2212 GGGCCC
AvaI 81 CTCGGG C^[CT]CG[GA]G
2536 CCCGGG
AvrII 949 CCTAGG C^CTAGG
1811 CCTAGG
BbvCI 329 GCTGAGG CCTCAGC(-5/-2)
916 GCTGAGG
BglI 1052 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1411 GCCCAGTGGGC
BlnI 949 CCTAGG C^CTAGG
1811 CCTAGG
BsaHI 89 GGCGCC G[GA]^CG[CT]C
124 GGCGTC
BsaI 389 GAGACC GGTCTC(1/5)
1739 GAGACC
BsaWI 714 ACCGGT [AT]^CCGG[AT]
1164 ACCGGA
BsgI 2751 CTGCAC GTGCAG(16/14)
1544 GTGCAG
BsmBI 379 CGTCTC CGTCTC(1/5)
2770 GAGACG
BsoBI 81 CTCGGG C^[CT]CG[GA]G
2536 CCCGGG
BspMI 278 ACCTGC ACCTGC(4/8)
2549 GCAGGT
BsrBI 1255 CCGCTC CCGCTC(-3/-3)
2276 GAGCGG
BsrDI 1486 CATTGC GCAATG(2/0)
2787 CATTGC
BstUI 25 CGCG CG^CG
832 CGCG
Bsu36I 625 CCTCAGG CC^T[ACGT]AGG
2462 CCTCAGG
DraIII 1760 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2161 CACCCGGTG
EaeI 1425 TGGCCA [CT]^GGCC[GA]
1866 CGGCCA
Eco47III 181 AGCGCT AGC^GCT
1920 AGCGCT
Eco81I 625 CCTCAGG CC^T[ACGT]AGG
2462 CCTCAGG
Hin1I 89 GGCGCC G[GA]^CG[CT]C
124 GGCGTC
HincII 434 GTCAAC GT[CT]^[GA]AC
620 GTCAAC
HindII 434 GTCAAC GT[CT]^[GA]AC
620 GTCAAC
MvnI 25 CGCG CG^CG
832 CGCG
NaeI 1892 GCCGGC GCC^GGC
2531 GCCGGC
NgoMIV 1892 GCCGGC G^CCGGC
2531 GCCGGC
SacI 211 GAGCTC GAGCT^C
557 GAGCTC
SfcI 199 CTGCAG C^T[GA][CT]AG
1377 CTGTAG
TaqI 798 TCGA T^CGA
997 TCGA
TthHB8I 798 TCGA T^CGA
997 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII