##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AviII AvrII
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI BspDI BspEI BspHI
BsrDI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NdeI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1259 GTCTAC GT^[AC][GT]AC
Alw44I 953 GTGCAC G^TGCAC
AocI 436 CCTCAGG CC^T[ACGT]AGG
ApaLI 953 GTGCAC G^TGCAC
AspI 788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 573 GGATCC G^GATCC
BbrPI 923 CACGTG CAC^GTG
BbsI 2599 GTCTTC GAAGAC(2/6)
BfaI 2110 CTAG C^TAG
BpuAI 2599 GTCTTC GAAGAC(2/6)
BsmI 853 GAATGC GAATGC(1/-1)
Bsp1407I 1464 TGTACA T^GTACA
BspLU11I 945 ACATGT A^CATGT
BsrGI 1464 TGTACA T^GTACA
BstEII 1204 GGTGACC G^GT[ACGT]ACC
BstPI 1204 GGTGACC G^GT[ACGT]ACC
BstXI 2037 CCAGGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 436 CCTCAGG CC^T[ACGT]AGG
DraIII 458 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 436 CCTCAGG CC^T[ACGT]AGG
EcoNI 292 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1204 GGTGACC G^GT[ACGT]ACC
FseI 1723 GGCCGGCC GGCCGG^CC
MaeI 2110 CTAG C^TAG
NheI 2109 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 886 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 923 CACGTG CAC^GTG
PmlI 923 CACGTG CAC^GTG
SapI 754 GAAGAGC GCTCTTC(1/4)
SspBI 1464 TGTACA T^GTACA
Tsp509I 1942 AATT ^AATT
Tth111I 788 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 886 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 922 ACACGT A^C[GA][CT]GT
945 ACATGT
AhdI 647 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTGGAGGTC
AscI 982 GGCGCGCC GG^CGCGCC
1709 GGCGCGCC
AspEI 647 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTGGAGGTC
BalI 468 TGGCCA TGG^CCA
1560 TGGCCA
BbeI 1061 GGCGCC GGCGC^C
2415 GGCGCC
BsaAI 632 TACGTG [CT]AC^GT[GA]
923 CACGTG
BsaI 1335 GAGACC GGTCTC(1/5)
2501 GAGACC
BsaWI 750 ACCGGA [AT]^CCGG[AT]
768 ACCGGA
BseRI 299 GAGGAG GAGGAG(10/8)
2558 GAGGAG
BsmBI 638 CGTCTC CGTCTC(1/5)
776 GAGACG
BsrBI 1490 CCGCTC CCGCTC(-3/-3)
1666 GAGCGG
BssSI 402 CACGAG CACGAG(-5/-1)
1127 CTCGTG
BstYI 573 GGATCC [GA]^GATC[CT]
2259 GGATCT
Eam1105I 647 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTGGAGGTC
EarI 754 GAAGAG CTCTTC(1/4)
2567 GAAGAG
KasI 1061 GGCGCC G^GCGCC
2415 GGCGCC
Ksp632I 754 GAAGAG CTCTTC(1/4)
2567 GAAGAG
MflI 573 GGATCC [GA]^GATC[CT]
2259 GGATCT
MluNI 468 TGGCCA TGG^CCA
1560 TGGCCA
MscI 468 TGGCCA TGG^CCA
1560 TGGCCA
NaeI 317 GCCGGC GCC^GGC
1724 GCCGGC
NarI 1061 GGCGCC GG^CGCC
2415 GGCGCC
NcoI 1963 CCATGG C^CATGG
2043 CCATGG
NgoMIV 317 GCCGGC G^CCGGC
1724 GCCGGC
NspI 873 ACATGC [GA]CATG^[CT]
945 ACATGT
SacI 232 GAGCTC GAGCT^C
1139 GAGCTC
SfoI 1061 GGCGCC GGC^GCC
2415 GGCGCC
SmlI 815 CTCAAG C^T[CT][GA]AG
2096 CTCAAG
XcmI 2002 CCACACCCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2461 CCAACAGCTGCTTGG
XhoII 573 GGATCC [GA]^GATC[CT]
2259 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI