##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AclI AcsI AgeI ApoI
AseI AsnI AvrII BciVI BclI
BlnI BsaBI BseAI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoRV FbaI
HpaI MamI MluI MroI NotI
NruI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SfiI
SfuI SgrAI SnaBI SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 812 GACGTC GACGT^C
Acc65I 945 GGTACC G^GTACC
AflII 3031 CTTAAG C^TTAAG
AhdI 1379 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 105 GGCGCGCC GG^CGCGCC
Asp718I 945 GGTACC G^GTACC
AspEI 1379 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4088 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3323 TGCGCA TGC^GCA
BcgI 2256 CGAGTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3031 CTTAAG C^TTAAG
BsiWI 2100 CGTACG C^GTACG
BstEII 3973 GGTGACC G^GT[ACGT]ACC
BstPI 3973 GGTGACC G^GT[ACGT]ACC
CpoI 4306 CGGTCCG CG^G[AT]CCG
DraIII 4181 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1379 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3973 GGTGACC G^GT[ACGT]ACC
FspI 3323 TGCGCA TGC^GCA
HincII 2767 GTCGAC GT[CT]^[GA]AC
HindII 2767 GTCGAC GT[CT]^[GA]AC
HindIII 1970 AAGCTT A^AGCTT
KpnI 945 GGTACC GGTAC^C
KspI 420 CCGCGG CCGC^GG
MfeI 3986 CAATTG C^AATTG
MunI 3986 CAATTG C^AATTG
NdeI 5376 CATATG CA^TATG
PaeR7I 1706 CTCGAG C^TCGAG
PflFI 4088 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 4306 CGGTCCG CG^G[AT]CCG
SacI 833 GAGCTC GAGCT^C
SacII 420 CCGCGG CCGC^GG
SalI 2767 GTCGAC G^TCGAC
SapI 907 GCTCTTC GCTCTTC(1/4)
SbfI 4542 CCTGCAGG CCTGCA^GG
ScaI 879 AGTACT AGT^ACT
SexAI 411 ACCAGGT A^CC[AT]GGT
SpeI 4031 ACTAGT A^CTAGT
Sse8387I 4542 CCTGCAGG CCTGCA^GG
SspI 5425 AATATT AAT^ATT
Tth111I 4088 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1706 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1476 GTAGAC GT^[AC][GT]AC
2767 GTCGAC
Asp700I 325 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4241 GAAGATCTTC
BglII 4243 AGATCT A^GATCT
4680 AGATCT
BsaI 4086 GAGACC GGTCTC(1/5)
4448 GGTCTC
BsaWI 1836 ACCGGA [AT]^CCGG[AT]
2466 TCCGGT
BsmI 2002 GCATTC GAATGC(1/-1)
3640 GCATTC
BssHII 40 GCGCGC G^CGCGC
106 GCGCGC
BssSI 2339 CACGAG CACGAG(-5/-1)
1025 CTCGTG
DrdI 1901 GACTCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4980 GACCCAGTGGTC
EagI 221 CGGCCG C^GGCCG
3046 CGGCCG
EarI 908 CTCTTC CTCTTC(1/4)
3063 CTCTTC
EclXI 221 CGGCCG C^GGCCG
3046 CGGCCG
Eco52I 221 CGGCCG C^GGCCG
3046 CGGCCG
EcoNI 949 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4912 CCTTCCTCAGG
EcoT22I 4938 ATGCAT ATGCA^T
5379 ATGCAT
FseI 610 GGCCGGCC GGCCGG^CC
4364 GGCCGGCC
Ksp632I 908 CTCTTC CTCTTC(1/4)
3063 CTCTTC
NheI 187 GCTAGC G^CTAGC
4533 GCTAGC
NsiI 4938 ATGCAT ATGCA^T
5379 ATGCAT
XmnI 325 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4241 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco47III Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI