##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AflII Alw44I ApaI ApaLI AscI
AvrII BbrPI BfrI BlnI BsiWI
BspDI BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I ClaI DraIII EcoO65I
EcoRI EcoRV MluI NdeI NheI
NotI PmaCI PmeI PmlI PvuI
SbfI ScaI SfiI Sse8387I XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1288 GACGTC GACGT^C
Acc65I 4555 GGTACC G^GTACC
AccIII 4303 TCCGGA T^CCGGA
AclI 2680 AACGTT AA^CGTT
AgeI 823 ACCGGT A^CCGGT
AhdI 357 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2046 CCTCAGG CC^T[ACGT]AGG
Aor51HI 222 AGCGCT AGC^GCT
AseI 4834 ATTAAT AT^TAAT
AsnI 4834 ATTAAT AT^TAAT
Asp718I 4555 GGTACC G^GTACC
AspEI 357 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4438 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1006 TGCGCA TGC^GCA
BamHI 5377 GGATCC G^GATCC
BglI 595 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4520 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 326 GAGACC GGTCTC(1/5)
BseAI 4303 TCCGGA T^CCGGA
BsmI 1919 GCATTC GAATGC(1/-1)
BspEI 4303 TCCGGA T^CCGGA
BstBI 1206 TTCGAA TT^CGAA
Bsu36I 2046 CCTCAGG CC^T[ACGT]AGG
CpoI 4171 CGGACCG CG^G[AT]CCG
Eam1105I 357 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 222 AGCGCT AGC^GCT
Eco81I 2046 CCTCAGG CC^T[ACGT]AGG
EcoNI 1031 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4735 ATGCAT ATGCA^T
FseI 609 GGCCGGCC GGCCGG^CC
FspI 1006 TGCGCA TGC^GCA
HindIII 2982 AAGCTT A^AGCTT
KpnI 4555 GGTACC GGTAC^C
KspI 1101 CCGCGG CCGC^GG
MamI 4520 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4303 TCCGGA T^CCGGA
NcoI 2630 CCATGG C^CATGG
NruI 173 TCGCGA TCG^CGA
NsiI 4735 ATGCAT ATGCA^T
NspV 1206 TTCGAA TT^CGAA
PaeR7I 108 CTCGAG C^TCGAG
PflFI 4438 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 854 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 823 ACCGGT A^CCGGT
PshBI 4834 ATTAAT AT^TAAT
Psp1406I 2680 AACGTT AA^CGTT
RsrII 4171 CGGACCG CG^G[AT]CCG
SacII 1101 CCGCGG CCGC^GG
SalI 2761 GTCGAC G^TCGAC
SapI 914 GAAGAGC GCTCTTC(1/4)
SexAI 4161 ACCTGGT A^CC[AT]GGT
SfuI 1206 TTCGAA TT^CGAA
SgrAI 506 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 108 CTCGAG C^T[CT][GA]AG
SnaBI 4823 TACGTA TAC^GTA
SpeI 3011 ACTAGT A^CTAGT
Tth111I 4438 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 854 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 108 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 801 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3204 CAGCTGCTG
Asp700I 1730 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3021 GAAGTTCTTC
BbvCI 1031 CCTCAGC CCTCAGC(-5/-2)
2286 CCTCAGC
BcgI 2526 CGACATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
220 GCAGCGCTCTCG
BciVI 1341 GTATCC GTATCC(6/5)
1425 GTATCC
BclI 1649 TGATCA T^GATCA
4124 TGATCA
BlpI 212 GCTGAGC GC^T[ACGT]AGC
1049 GCTGAGC
Bpu1102I 212 GCTGAGC GC^T[ACGT]AGC
1049 GCTGAGC
BspHI 877 TCATGA T^CATGA
5071 TCATGA
BspLU11I 1360 ACATGT A^CATGT
3059 ACATGT
BsrDI 5305 CATTGC GCAATG(2/0)
3244 GCAATG
CelII 212 GCTGAGC GC^T[ACGT]AGC
1049 GCTGAGC
DrdI 3119 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4412 GACCATTTGGTC
FbaI 1649 TGATCA T^GATCA
4124 TGATCA
HpaI 3447 GTTAAC GTT^AAC
4966 GTTAAC
PacI 4810 TTAATTAA TTAAT^TAA
4835 TTAATTAA
PshAI 2246 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4052 GACTTTCGTC
RcaI 877 TCATGA T^CATGA
5071 TCATGA
SacI 1052 GAGCTC GAGCT^C
2326 GAGCTC
SphI 1417 GCATGC GCATG^C
3110 GCATGC
SspI 5181 AATATT AAT^ATT
5448 AATATT
StuI 1574 AGGCCT AGG^CCT
2640 AGGCCT
SwaI 5184 ATTTAAAT ATTT^AAAT
5260 ATTTAAAT
XmnI 1730 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3021 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI