################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AccIII AclI AflII AscI AseI AsnI AspI BcgI BciVI BclI BfrI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI MluI MroI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1887 GACGTC GACGT^C Acc65I 387 GGTACC G^GTACC AccI 350 GTCTAC GT^[AC][GT]AC AgeI 4022 ACCGGT A^CCGGT Alw44I 1764 GTGCAC G^TGCAC Aor51HI 165 AGCGCT AGC^GCT ApaLI 1764 GTGCAC G^TGCAC Asp718I 387 GGTACC G^GTACC AvaI 2303 CCCGGG C^[CT]CG[GA]G AviII 2107 TGCGCA TGC^GCA BamHI 4427 GGATCC G^GATCC BbeI 1824 GGCGCC GGCGC^C BbrPI 1050 CACGTG CAC^GTG BglI 5238 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3696 AGATCT A^GATCT BsaBI 4416 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4022 ACCGGT [AT]^CCGG[AT] BsoBI 2303 CCCGGG C^[CT]CG[GA]G BsrDI 3313 GCAATG GCAATG(2/0) BsrFI 4022 ACCGGT [GA]^CCGG[CT] Cfr10I 4022 ACCGGT [GA]^CCGG[CT] Eco47III 165 AGCGCT AGC^GCT FspI 2107 TGCGCA TGC^GCA KasI 1824 GGCGCC G^GCGCC KpnI 387 GGTACC GGTAC^C MamI 4416 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3228 CAATTG C^AATTG MunI 3228 CAATTG C^AATTG NarI 1824 GGCGCC GG^CGCC PinAI 4022 ACCGGT A^CCGGT PmaCI 1050 CACGTG CAC^GTG PmlI 1050 CACGTG CAC^GTG ScaI 2254 AGTACT AGT^ACT SfoI 1824 GGCGCC GGC^GCC SgrAI 4021 CACCGGTG C[GA]^CCGG[CT]G SmaI 2303 CCCGGG CCC^GGG SphI 2870 GCATGC GCATG^C TaqI 278 TCGA T^CGA TthHB8I 278 TCGA T^CGA XmaCI 2303 CCCGGG C^CCGGG XmaI 2303 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 1675 ACATGT A^C[GA][CT]GT 3795 ACATGT AvrII 2273 CCTAGG C^CTAGG 4160 CCTAGG BlnI 2273 CCTAGG C^CTAGG 4160 CCTAGG BsaAI 1050 CACGTG [CT]AC^GT[GA] 2361 CACGTA BsmBI 138 CGTCTC CGTCTC(1/5) 3275 GAGACG BspLU11I 1675 ACATGT A^CATGT 3795 ACATGT BssSI 3381 CACGAG CACGAG(-5/-1) 1843 CTCGTG HaeII 165 AGCGCT [GA]GCGC^[CT] 1824 GGCGCC HgaI 151 GACGC GACGC(5/10) 3277 GACGC HindIII 1223 AAGCTT A^AGCTT 2637 AAGCTT KspI 271 CCGCGG CCGC^GG 2026 CCGCGG NdeI 1932 CATATG CA^TATG 2115 CATATG PflMI 4206 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4739 CCAGGTGATGG SacII 271 CCGCGG CCGC^GG 2026 CCGCGG Van91I 4206 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4739 CCAGGTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspEI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI