##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AccIII      AclI        AflII       AscI        AseI        
AsnI        AspI        BcgI        BciVI       BclI        
BfrI        BsaI        BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
MluI        MroI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        SexAI       SfiI        SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        1887  GACGTC               GACGT^C
Acc65I        387  GGTACC               G^GTACC
AccI          350  GTCTAC               GT^[AC][GT]AC
AgeI         4022  ACCGGT               A^CCGGT
Alw44I       1764  GTGCAC               G^TGCAC
Aor51HI       165  AGCGCT               AGC^GCT
ApaLI        1764  GTGCAC               G^TGCAC
Asp718I       387  GGTACC               G^GTACC
AvaI         2303  CCCGGG               C^[CT]CG[GA]G
AviII        2107  TGCGCA               TGC^GCA
BamHI        4427  GGATCC               G^GATCC
BbeI         1824  GGCGCC               GGCGC^C
BbrPI        1050  CACGTG               CAC^GTG
BglI         5238  GCCAATGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3696  AGATCT               A^GATCT
BsaBI        4416  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4022  ACCGGT               [AT]^CCGG[AT]
BsoBI        2303  CCCGGG               C^[CT]CG[GA]G
BsrDI        3313  GCAATG               GCAATG(2/0)
BsrFI        4022  ACCGGT               [GA]^CCGG[CT]
Cfr10I       4022  ACCGGT               [GA]^CCGG[CT]
Eco47III      165  AGCGCT               AGC^GCT
FspI         2107  TGCGCA               TGC^GCA
KasI         1824  GGCGCC               G^GCGCC
KpnI          387  GGTACC               GGTAC^C
MamI         4416  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3228  CAATTG               C^AATTG
MunI         3228  CAATTG               C^AATTG
NarI         1824  GGCGCC               GG^CGCC
PinAI        4022  ACCGGT               A^CCGGT
PmaCI        1050  CACGTG               CAC^GTG
PmlI         1050  CACGTG               CAC^GTG
ScaI         2254  AGTACT               AGT^ACT
SfoI         1824  GGCGCC               GGC^GCC
SgrAI        4021  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2303  CCCGGG               CCC^GGG
SphI         2870  GCATGC               GCATG^C
TaqI          278  TCGA                 T^CGA
TthHB8I       278  TCGA                 T^CGA
XmaCI        2303  CCCGGG               C^CCGGG
XmaI         2303  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AflIII       1675  ACATGT               A^C[GA][CT]GT
             3795  ACATGT              
AvrII        2273  CCTAGG               C^CTAGG
             4160  CCTAGG              
BlnI         2273  CCTAGG               C^CTAGG
             4160  CCTAGG              
BsaAI        1050  CACGTG               [CT]AC^GT[GA]
             2361  CACGTA              
BsmBI         138  CGTCTC               CGTCTC(1/5)
             3275  GAGACG              
BspLU11I     1675  ACATGT               A^CATGT
             3795  ACATGT              
BssSI        3381  CACGAG               CACGAG(-5/-1)
             1843  CTCGTG              
HaeII         165  AGCGCT               [GA]GCGC^[CT]
             1824  GGCGCC              
HgaI          151  GACGC                GACGC(5/10)
             3277  GACGC               
HindIII      1223  AAGCTT               A^AGCTT
             2637  AAGCTT              
KspI          271  CCGCGG               CCGC^GG
             2026  CCGCGG              
NdeI         1932  CATATG               CA^TATG
             2115  CATATG              
PflMI        4206  CCAGCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4739  CCAGGTGATGG         
SacII         271  CCGCGG               CCGC^GG
             2026  CCGCGG              
Van91I       4206  CCAGCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4739  CCAGGTGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        Asp700I     AspEI       AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eam1105I    EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XcmI        XhoII       XmnI