################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AatII Acc65I AgeI AhdI AscI Asp718I AspEI AvrII BbvCI BlnI BsiWI BspDI BssHII BssSI BstAPI BstBI BstEII BstPI ClaI CpoI DraIII Eam1105I EcoO65I FseI KpnI KspI NheI NotI NruI NspV PacI PinAI PmeI PvuI RsrII SacII SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 6542 AACGTT AA^CGTT Alw44I 6584 GTGCAC G^TGCAC Aor51HI 5264 AGCGCT AGC^GCT ApaI 195 GGGCCC GGGCC^C ApaLI 6584 GTGCAC G^TGCAC AspI 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5239 TGCGCA TGC^GCA BanI 3760 GGCGCC G^G[CT][GA]CC BbeI 3760 GGCGCC GGCGC^C BbrPI 2662 CACGTG CAC^GTG BglII 740 AGATCT A^GATCT BsaBI 3480 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 4496 ACATGT A^CATGT BsrBI 4490 GAGCGG CCGCTC(-3/-3) EagI 5981 CGGCCG C^GGCCG EclXI 5981 CGGCCG C^GGCCG Eco47III 5264 AGCGCT AGC^GCT Eco52I 5981 CGGCCG C^GGCCG EcoRV 6098 GATATC GAT^ATC FspI 5239 TGCGCA TGC^GCA HpaI 2743 GTTAAC GTT^AAC KasI 3760 GGCGCC G^GCGCC MamI 3480 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1841 CAATTG C^AATTG MluI 2828 ACGCGT A^CGCGT MunI 1841 CAATTG C^AATTG NaeI 204 GCCGGC GCC^GGC NarI 3760 GGCGCC GG^CGCC NcoI 4344 CCATGG C^CATGG NgoMIV 204 GCCGGC G^CCGGC PflFI 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2662 CACGTG CAC^GTG PmlI 2662 CACGTG CAC^GTG Psp1406I 6542 AACGTT AA^CGTT SalI 553 GTCGAC G^TCGAC SexAI 2139 ACCTGGT A^CC[AT]GGT SfoI 3760 GGCGCC GGC^GCC SmaI 192 CCCGGG CCC^GGG Tth111I 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1830 TCTAGA T^CTAGA XcmI 4950 CCATGTCAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 192 CCCGGG C^CCGGG XmaI 192 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 2472 TCCGGA T^CCGGA 4631 TCCGGA AcyI 3708 GACGCC G[GA]^CG[CT]C 3760 GGCGCC AflIII 2828 ACGCGT A^C[GA][CT]GT 4496 ACATGT AseI 7039 ATTAAT AT^TAAT 7425 ATTAAT AsnI 7039 ATTAAT AT^TAAT 7425 ATTAAT Asp700I 1338 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1463 GAATATTTTC BcgI 349 CGATACATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5271 CGAGCTCATTGC BciVI 4802 GGATAC GTATCC(6/5) 1449 GTATCC BclI 2978 TGATCA T^GATCA 5996 TGATCA BsaHI 3708 GACGCC G[GA]^CG[CT]C 3760 GGCGCC BseAI 2472 TCCGGA T^CCGGA 4631 TCCGGA BsiEI 5981 CGGCCG CG[GA][CT]^CG 6400 CGGTCG BsmBI 336 GAGACG CGTCTC(1/5) 672 GAGACG BspEI 2472 TCCGGA T^CCGGA 4631 TCCGGA BspHI 1431 TCATGA T^CATGA 4361 TCATGA BsrFI 204 GCCGGC [GA]^CCGG[CT] 4748 ACCGGC Bst1107I 2541 GTATAC GTA^TAC 7272 GTATAC BstZ17I 2541 GTATAC GTA^TAC 7272 GTATAC Cfr10I 204 GCCGGC [GA]^CCGG[CT] 4748 ACCGGC DsaI 3941 CCGTGG C^C[GA][CT]GG 4344 CCATGG EcoNI 3741 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5081 CCTGCCTGAGG EcoRI 2083 GAATTC G^AATTC 6323 GAATTC FauI 3652 GCGGG CCCGC(4/6) 5807 GCGGG FbaI 2978 TGATCA T^GATCA 5996 TGATCA Hin1I 3708 GACGCC G[GA]^CG[CT]C 3760 GGCGCC MroI 2472 TCCGGA T^CCGGA 4631 TCCGGA PaeR7I 6205 CTCGAG C^TCGAG 6792 CTCGAG PshBI 7039 ATTAAT AT^TAAT 7425 ATTAAT RcaI 1431 TCATGA T^CATGA 4361 TCATGA SpeI 6874 ACTAGT A^CTAGT 7007 ACTAGT XhoI 6205 CTCGAG C^TCGAG 6792 CTCGAG XmnI 1338 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1463 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII