##################################
# NO CUT ENZYMES (   43 enzymes) #
##################################

AatII       Acc65I      AgeI        AhdI        AscI        
Asp718I     AspEI       AvrII       BbvCI       BlnI        
BsiWI       BspDI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       ClaI        CpoI        
DraIII      Eam1105I    EcoO65I     FseI        KpnI        
KspI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        PvuI        RsrII       
SacII       SbfI        SfiI        SfuI        SgrAI       
SnaBI       Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AclI         6542  AACGTT               AA^CGTT
Alw44I       6584  GTGCAC               G^TGCAC
Aor51HI      5264  AGCGCT               AGC^GCT
ApaI          195  GGGCCC               GGGCC^C
ApaLI        6584  GTGCAC               G^TGCAC
AspI          341  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        5239  TGCGCA               TGC^GCA
BanI         3760  GGCGCC               G^G[CT][GA]CC
BbeI         3760  GGCGCC               GGCGC^C
BbrPI        2662  CACGTG               CAC^GTG
BglII         740  AGATCT               A^GATCT
BsaBI        3480  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I     4496  ACATGT               A^CATGT
BsrBI        4490  GAGCGG               CCGCTC(-3/-3)
EagI         5981  CGGCCG               C^GGCCG
EclXI        5981  CGGCCG               C^GGCCG
Eco47III     5264  AGCGCT               AGC^GCT
Eco52I       5981  CGGCCG               C^GGCCG
EcoRV        6098  GATATC               GAT^ATC
FspI         5239  TGCGCA               TGC^GCA
HpaI         2743  GTTAAC               GTT^AAC
KasI         3760  GGCGCC               G^GCGCC
MamI         3480  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1841  CAATTG               C^AATTG
MluI         2828  ACGCGT               A^CGCGT
MunI         1841  CAATTG               C^AATTG
NaeI          204  GCCGGC               GCC^GGC
NarI         3760  GGCGCC               GG^CGCC
NcoI         4344  CCATGG               C^CATGG
NgoMIV        204  GCCGGC               G^CCGGC
PflFI         341  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2662  CACGTG               CAC^GTG
PmlI         2662  CACGTG               CAC^GTG
Psp1406I     6542  AACGTT               AA^CGTT
SalI          553  GTCGAC               G^TCGAC
SexAI        2139  ACCTGGT              A^CC[AT]GGT
SfoI         3760  GGCGCC               GGC^GCC
SmaI          192  CCCGGG               CCC^GGG
Tth111I       341  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1830  TCTAGA               T^CTAGA
XcmI         4950  CCATGTCAAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         192  CCCGGG               C^CCGGG
XmaI          192  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII       2472  TCCGGA               T^CCGGA
             4631  TCCGGA              
AcyI         3708  GACGCC               G[GA]^CG[CT]C
             3760  GGCGCC              
AflIII       2828  ACGCGT               A^C[GA][CT]GT
             4496  ACATGT              
AseI         7039  ATTAAT               AT^TAAT
             7425  ATTAAT              
AsnI         7039  ATTAAT               AT^TAAT
             7425  ATTAAT              
Asp700I      1338  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1463  GAATATTTTC          
BcgI          349  CGATACATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5271  CGAGCTCATTGC        
BciVI        4802  GGATAC               GTATCC(6/5)
             1449  GTATCC              
BclI         2978  TGATCA               T^GATCA
             5996  TGATCA              
BsaHI        3708  GACGCC               G[GA]^CG[CT]C
             3760  GGCGCC              
BseAI        2472  TCCGGA               T^CCGGA
             4631  TCCGGA              
BsiEI        5981  CGGCCG               CG[GA][CT]^CG
             6400  CGGTCG              
BsmBI         336  GAGACG               CGTCTC(1/5)
              672  GAGACG              
BspEI        2472  TCCGGA               T^CCGGA
             4631  TCCGGA              
BspHI        1431  TCATGA               T^CATGA
             4361  TCATGA              
BsrFI         204  GCCGGC               [GA]^CCGG[CT]
             4748  ACCGGC              
Bst1107I     2541  GTATAC               GTA^TAC
             7272  GTATAC              
BstZ17I      2541  GTATAC               GTA^TAC
             7272  GTATAC              
Cfr10I        204  GCCGGC               [GA]^CCGG[CT]
             4748  ACCGGC              
DsaI         3941  CCGTGG               C^C[GA][CT]GG
             4344  CCATGG              
EcoNI        3741  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5081  CCTGCCTGAGG         
EcoRI        2083  GAATTC               G^AATTC
             6323  GAATTC              
FauI         3652  GCGGG                CCCGC(4/6)
             5807  GCGGG               
FbaI         2978  TGATCA               T^GATCA
             5996  TGATCA              
Hin1I        3708  GACGCC               G[GA]^CG[CT]C
             3760  GGCGCC              
MroI         2472  TCCGGA               T^CCGGA
             4631  TCCGGA              
PaeR7I       6205  CTCGAG               C^TCGAG
             6792  CTCGAG              
PshBI        7039  ATTAAT               AT^TAAT
             7425  ATTAAT              
RcaI         1431  TCATGA               T^CATGA
             4361  TCATGA              
SpeI         6874  ACTAGT               A^CTAGT
             7007  ACTAGT              
XhoI         6205  CTCGAG               C^TCGAG
             6792  CTCGAG              
XmnI         1338  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1463  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BalI        
BamHI       BanII       BbsI        BbvI        BcnI        
BfaI        BfrI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DrdI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SphI        SspBI       SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII