##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII Acc65I AgeI AhdI AscI
Asp718I AspEI AvrII BbvCI BlnI
BsiWI BspDI BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII Eam1105I EcoO65I FseI KpnI
KspI NheI NotI NruI NspV
PacI PinAI PmeI PvuI RsrII
SacII SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 6542 AACGTT AA^CGTT
Alw44I 6584 GTGCAC G^TGCAC
Aor51HI 5264 AGCGCT AGC^GCT
ApaI 195 GGGCCC GGGCC^C
ApaLI 6584 GTGCAC G^TGCAC
AspI 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5239 TGCGCA TGC^GCA
BanI 3760 GGCGCC G^G[CT][GA]CC
BbeI 3760 GGCGCC GGCGC^C
BbrPI 2662 CACGTG CAC^GTG
BglII 740 AGATCT A^GATCT
BsaBI 3480 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4496 ACATGT A^CATGT
BsrBI 4490 GAGCGG CCGCTC(-3/-3)
EagI 5981 CGGCCG C^GGCCG
EclXI 5981 CGGCCG C^GGCCG
Eco47III 5264 AGCGCT AGC^GCT
Eco52I 5981 CGGCCG C^GGCCG
EcoRV 6098 GATATC GAT^ATC
FspI 5239 TGCGCA TGC^GCA
HpaI 2743 GTTAAC GTT^AAC
KasI 3760 GGCGCC G^GCGCC
MamI 3480 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1841 CAATTG C^AATTG
MluI 2828 ACGCGT A^CGCGT
MunI 1841 CAATTG C^AATTG
NaeI 204 GCCGGC GCC^GGC
NarI 3760 GGCGCC GG^CGCC
NcoI 4344 CCATGG C^CATGG
NgoMIV 204 GCCGGC G^CCGGC
PflFI 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2662 CACGTG CAC^GTG
PmlI 2662 CACGTG CAC^GTG
Psp1406I 6542 AACGTT AA^CGTT
SalI 553 GTCGAC G^TCGAC
SexAI 2139 ACCTGGT A^CC[AT]GGT
SfoI 3760 GGCGCC GGC^GCC
SmaI 192 CCCGGG CCC^GGG
Tth111I 341 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1830 TCTAGA T^CTAGA
XcmI 4950 CCATGTCAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 192 CCCGGG C^CCGGG
XmaI 192 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2472 TCCGGA T^CCGGA
4631 TCCGGA
AcyI 3708 GACGCC G[GA]^CG[CT]C
3760 GGCGCC
AflIII 2828 ACGCGT A^C[GA][CT]GT
4496 ACATGT
AseI 7039 ATTAAT AT^TAAT
7425 ATTAAT
AsnI 7039 ATTAAT AT^TAAT
7425 ATTAAT
Asp700I 1338 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1463 GAATATTTTC
BcgI 349 CGATACATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5271 CGAGCTCATTGC
BciVI 4802 GGATAC GTATCC(6/5)
1449 GTATCC
BclI 2978 TGATCA T^GATCA
5996 TGATCA
BsaHI 3708 GACGCC G[GA]^CG[CT]C
3760 GGCGCC
BseAI 2472 TCCGGA T^CCGGA
4631 TCCGGA
BsiEI 5981 CGGCCG CG[GA][CT]^CG
6400 CGGTCG
BsmBI 336 GAGACG CGTCTC(1/5)
672 GAGACG
BspEI 2472 TCCGGA T^CCGGA
4631 TCCGGA
BspHI 1431 TCATGA T^CATGA
4361 TCATGA
BsrFI 204 GCCGGC [GA]^CCGG[CT]
4748 ACCGGC
Bst1107I 2541 GTATAC GTA^TAC
7272 GTATAC
BstZ17I 2541 GTATAC GTA^TAC
7272 GTATAC
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
4748 ACCGGC
DsaI 3941 CCGTGG C^C[GA][CT]GG
4344 CCATGG
EcoNI 3741 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5081 CCTGCCTGAGG
EcoRI 2083 GAATTC G^AATTC
6323 GAATTC
FauI 3652 GCGGG CCCGC(4/6)
5807 GCGGG
FbaI 2978 TGATCA T^GATCA
5996 TGATCA
Hin1I 3708 GACGCC G[GA]^CG[CT]C
3760 GGCGCC
MroI 2472 TCCGGA T^CCGGA
4631 TCCGGA
PaeR7I 6205 CTCGAG C^TCGAG
6792 CTCGAG
PshBI 7039 ATTAAT AT^TAAT
7425 ATTAAT
RcaI 1431 TCATGA T^CATGA
4361 TCATGA
SpeI 6874 ACTAGT A^CTAGT
7007 ACTAGT
XhoI 6205 CTCGAG C^TCGAG
6792 CTCGAG
XmnI 1338 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1463 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanII BbsI BbvI BcnI
BfaI BfrI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DrdI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII