##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AseI AsnI Asp718I AspI AviII
AvrII BciVI BlnI BsiWI BsmBI
BspDI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eco47III
EcoO65I EcoRV FseI FspI HpaI
KpnI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SfiI SfuI SnaBI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflII 5217 CTTAAG C^TTAAG
AhdI 344 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4006 GGGCCC GGGCC^C
AscI 66 GGCGCGCC GG^CGCGCC
Asp700I 2381 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 344 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1342 GGATCC G^GATCC
BbvCI 5277 CCTCAGC CCTCAGC(-5/-2)
BcgI 781 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1063 TGATCA T^GATCA
BfrI 5217 CTTAAG C^TTAAG
BglII 2035 AGATCT A^GATCT
BssSI 2085 CTCGTG CACGAG(-5/-1)
DraIII 3769 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 344 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4957 GAATTC G^AATTC
FbaI 1063 TGATCA T^GATCA
HindIII 597 AAGCTT A^AGCTT
MluI 1623 ACGCGT A^CGCGT
PshAI 305 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1453 GAAGAGC GCTCTTC(1/4)
SbfI 1336 CCTGCAGG CCTGCA^GG
ScaI 1513 AGTACT AGT^ACT
SmaI 718 CCCGGG CCC^GGG
SphI 4359 GCATGC GCATG^C
Sse8387I 1336 CCTGCAGG CCTGCA^GG
XmaCI 718 CCCGGG C^CCGGG
XmaI 718 CCCGGG C^CCGGG
XmnI 2381 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 302 GTCGAC GT^[AC][GT]AC
923 GTCGAC
AccIII 280 TCCGGA T^CCGGA
1814 TCCGGA
AcsI 3497 AAATTT [GA]^AATT[CT]
4957 GAATTC
AlwNI 586 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1119 CAGAGCCTG
AocI 1906 CCTCAGG CC^T[ACGT]AGG
3789 CCTGAGG
ApoI 3497 AAATTT [GA]^AATT[CT]
4957 GAATTC
BbrPI 2868 CACGTG CAC^GTG
3908 CACGTG
BlpI 3598 GCTCAGC GC^T[ACGT]AGC
5364 GCTTAGC
BpmI 3578 CTCCAG CTGGAG(16/14)
5094 CTCCAG
Bpu1102I 3598 GCTCAGC GC^T[ACGT]AGC
5364 GCTTAGC
BsaBI 3683 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4846 GATATTTATC
BsaWI 280 TCCGGA [AT]^CCGG[AT]
1814 TCCGGA
BseAI 280 TCCGGA T^CCGGA
1814 TCCGGA
BsmI 3179 GAATGC GAATGC(1/-1)
4054 GCATTC
BspEI 280 TCCGGA T^CCGGA
1814 TCCGGA
BspHI 2230 TCATGA T^CATGA
3215 TCATGA
BstXI 2529 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3043 CCAGTCAACTGG
Bsu36I 1906 CCTCAGG CC^T[ACGT]AGG
3789 CCTGAGG
CelII 3598 GCTCAGC GC^T[ACGT]AGC
5364 GCTTAGC
DraI 3663 TTTAAA TTT^AAA
4918 TTTAAA
DrdI 2931 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3816 GACACGCGGGTC
EagI 94 CGGCCG C^GGCCG
676 CGGCCG
EclXI 94 CGGCCG C^GGCCG
676 CGGCCG
Eco52I 94 CGGCCG C^GGCCG
676 CGGCCG
Eco81I 1906 CCTCAGG CC^T[ACGT]AGG
3789 CCTGAGG
MamI 3683 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4846 GATATTTATC
MroI 280 TCCGGA T^CCGGA
1814 TCCGGA
PmaCI 2868 CACGTG CAC^GTG
3908 CACGTG
PmlI 2868 CACGTG CAC^GTG
3908 CACGTG
PvuII 2935 CAGCTG CAG^CTG
3552 CAGCTG
RcaI 2230 TCATGA T^CATGA
3215 TCATGA
SalI 302 GTCGAC G^TCGAC
923 GTCGAC
SexAI 1285 ACCTGGT A^CC[AT]GGT
1756 ACCAGGT
SgrAI 140 CGCCGGCG C[GA]^CCGG[CT]G
385 CACCGGCG
SmlI 3697 CTCAAG C^T[CT][GA]AG
5217 CTTAAG
SpeI 1446 ACTAGT A^CTAGT
3885 ACTAGT
StuI 1486 AGGCCT AGG^CCT
4558 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII