##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BamHI BcgI BciVI BfaI
BfrI BglII BlnI BsaBI BsgI
BsiHKAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstBI BstZ17I ClaI DraIII
DrdI EcoNI EcoRI EcoT22I FseI
FspI HpaI KpnI MaeI MamI
MfeI MluI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SacI SalI SapI ScaI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1971 GACGTC GACGT^C
AccIII 725 TCCGGA T^CCGGA
AcsI 1870 AAATTC [GA]^AATT[CT]
AflIII 1094 ACGTGT A^C[GA][CT]GT
AhdI 2176 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1870 AAATTC [GA]^AATT[CT]
AspEI 2176 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 391 GAAGAC GAAGAC(2/6)
BbvCI 1141 GCTGAGG CCTCAGC(-5/-2)
BlpI 957 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 957 GCTGAGC GC^T[ACGT]AGC
BpuAI 391 GAAGAC GAAGAC(2/6)
BseAI 725 TCCGGA T^CCGGA
BsiWI 2269 CGTACG C^GTACG
BspEI 725 TCCGGA T^CCGGA
BssHII 943 GCGCGC G^CGCGC
BstEII 1998 GGTGACC G^GT[ACGT]ACC
BstPI 1998 GGTGACC G^GT[ACGT]ACC
BstXI 1272 CCATAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1839 GGATCT [GA]^GATC[CT]
CelII 957 GCTGAGC GC^T[ACGT]AGC
CpoI 58 CGGACCG CG^G[AT]CCG
DraI 2204 TTTAAA TTT^AAA
Eam1105I 2176 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2071 GAAGAG CTCTTC(1/4)
EcoO65I 1998 GGTGACC G^GT[ACGT]ACC
EcoRV 835 GATATC GAT^ATC
HincII 2310 GTCAAC GT[CT]^[GA]AC
HindII 2310 GTCAAC GT[CT]^[GA]AC
HindIII 1246 AAGCTT A^AGCTT
Ksp632I 2071 GAAGAG CTCTTC(1/4)
KspI 1647 CCGCGG CCGC^GG
MflI 1839 GGATCT [GA]^GATC[CT]
MroI 725 TCCGGA T^CCGGA
MslI 1852 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 2429 GCGGCCGC GC^GGCCGC
PvuII 277 CAGCTG CAG^CTG
RsrII 58 CGGACCG CG^G[AT]CCG
SacII 1647 CCGCGG CCGC^GG
SbfI 1668 CCTGCAGG CCTGCA^GG
SphI 1546 GCATGC GCATG^C
Sse8387I 1668 CCTGCAGG CCTGCA^GG
StuI 155 AGGCCT AGG^CCT
TaqI 1778 TCGA T^CGA
Tsp509I 1871 AATT ^AATT
TthHB8I 1778 TCGA T^CGA
XhoII 1839 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 500 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1378 CAGGACCTG
Aor51HI 629 AGCGCT AGC^GCT
1679 AGCGCT
BalI 380 TGGCCA TGG^CCA
614 TGGCCA
BbeI 689 GGCGCC GGCGC^C
1032 GGCGCC
BbrPI 1093 CACGTG CAC^GTG
1361 CACGTG
BclI 1235 TGATCA T^GATCA
1700 TGATCA
BsaAI 1093 CACGTG [CT]AC^GT[GA]
1361 CACGTG
BsaI 1078 GAGACC GGTCTC(1/5)
480 GGTCTC
BsaWI 29 ACCGGA [AT]^CCGG[AT]
725 TCCGGA
BssSI 1568 CACGAG CACGAG(-5/-1)
1057 CTCGTG
Eco47III 629 AGCGCT AGC^GCT
1679 AGCGCT
FbaI 1235 TGATCA T^GATCA
1700 TGATCA
KasI 689 GGCGCC G^GCGCC
1032 GGCGCC
MluNI 380 TGGCCA TGG^CCA
614 TGGCCA
MscI 380 TGGCCA TGG^CCA
614 TGGCCA
NaeI 984 GCCGGC GCC^GGC
1009 GCCGGC
NarI 689 GGCGCC GG^CGCC
1032 GGCGCC
NgoMIV 984 GCCGGC G^CCGGC
1009 GCCGGC
PmaCI 1093 CACGTG CAC^GTG
1361 CACGTG
PmlI 1093 CACGTG CAC^GTG
1361 CACGTG
SfiI 372 GGCCCCTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1318 GGCCTGGGGGGCC
SfoI 689 GGCGCC GGC^GCC
1032 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TfiI
Tru9I TseI Tsp45I TspRI XmaCI
XmaI