##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AclI AcyI AgeI
AscI AviII BamHI BbeI BcgI
BglI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI BspDI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI EagI EclXI Eco52I
FseI FspI HgaI Hin1I HpaI
KasI KspI MluI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4216 GGTACC G^GTACC
AccII 91 CGCG CG^CG
AccIII 3005 TCCGGA T^CCGGA
AhdI 5658 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2050 GTGCAC G^TGCAC
Aor51HI 146 AGCGCT AGC^GCT
ApaI 2034 GGGCCC GGGCC^C
ApaLI 2050 GTGCAC G^TGCAC
Asp718I 4216 GGTACC G^GTACC
AspEI 5658 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1853 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 4216 GGTACC G^G[CT][GA]CC
BsaBI 4906 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3005 TCCGGA [AT]^CCGG[AT]
BseAI 3005 TCCGGA T^CCGGA
Bsp1407I 2001 TGTACA T^GTACA
BspEI 3005 TCCGGA T^CCGGA
BspMI 5491 GCAGGT ACCTGC(4/8)
BsrBI 314 GAGCGG CCGCTC(-3/-3)
BsrGI 2001 TGTACA T^GTACA
BstEII 4406 GGTGACC G^GT[ACGT]ACC
BstPI 4406 GGTGACC G^GT[ACGT]ACC
BstUI 91 CGCG CG^CG
DraIII 2897 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 73 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5658 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 146 AGCGCT AGC^GCT
EcoO65I 4406 GGTGACC G^GT[ACGT]ACC
EcoT22I 3089 ATGCAT ATGCA^T
HincII 218 GTTGAC GT[CT]^[GA]AC
HindII 218 GTTGAC GT[CT]^[GA]AC
KpnI 4216 GGTACC GGTAC^C
MamI 4906 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4611 CAATTG C^AATTG
MroI 3005 TCCGGA T^CCGGA
MunI 4611 CAATTG C^AATTG
MvnI 91 CGCG CG^CG
NheI 5010 GCTAGC G^CTAGC
NsiI 3089 ATGCAT ATGCA^T
PflFI 1853 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3454 GAGCTC GAGCT^C
SgrAI 2108 CACCGGCG C[GA]^CCGG[CT]G
SpeI 4877 ACTAGT A^CTAGT
SspBI 2001 TGTACA T^GTACA
Tth111I 1853 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 3292 CTTAAG C^TTAAG
3407 CTTAAG
AseI 2705 ATTAAT AT^TAAT
5037 ATTAAT
AsnI 2705 ATTAAT AT^TAAT
5037 ATTAAT
AvaI 909 CCCGAG C^[CT]CG[GA]G
1892 CCCGAG
AvrII 4364 CCTAGG C^CTAGG
4849 CCTAGG
BbrPI 2375 CACGTG CAC^GTG
4133 CACGTG
BbvCI 3486 CCTCAGC CCTCAGC(-5/-2)
5539 GCTGAGG
BciVI 2486 GGATAC GTATCC(6/5)
5229 GTATCC
BclI 1363 TGATCA T^GATCA
4494 TGATCA
BfrI 3292 CTTAAG C^TTAAG
3407 CTTAAG
BglII 1004 AGATCT A^GATCT
1354 AGATCT
BlnI 4364 CCTAGG C^CTAGG
4849 CCTAGG
BsaAI 2375 CACGTG [CT]AC^GT[GA]
4133 CACGTG
BsaI 1372 GGTCTC GGTCTC(1/5)
2636 GGTCTC
BsoBI 909 CCCGAG C^[CT]CG[GA]G
1892 CCCGAG
BspLU11I 690 ACATGT A^CATGT
2273 ACATGT
EcoRI 661 GAATTC G^AATTC
1252 GAATTC
EcoRV 3931 GATATC GAT^ATC
4224 GATATC
FauI 92 GCGGG CCCGC(4/6)
2020 GCGGG
FbaI 1363 TGATCA T^GATCA
4494 TGATCA
HindIII 2150 AAGCTT A^AGCTT
2350 AAGCTT
PmaCI 2375 CACGTG CAC^GTG
4133 CACGTG
PmlI 2375 CACGTG CAC^GTG
4133 CACGTG
PpuMI 1180 AGGACCT [GA]G^G[AT]CC[CT]
3214 AGGACCC
PshBI 2705 ATTAAT AT^TAAT
5037 ATTAAT
SapI 589 GAAGAGC GCTCTTC(1/4)
3456 GCTCTTC
XbaI 565 TCTAGA T^CTAGA
3505 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI