##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AccIII      AflIII      AhdI        
Aor51HI     AscI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BcgI        BlnI        BsaAI       BseAI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      ClaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      FspI        HindIII     KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        NarI        NheI        NotI        NruI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
SacII       SalI        SfiI        SfoI        SnaBI       
StuI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI           94  GGCGTC               G[GA]^CG[CT]C
AflII          46  CTTAAG               C^TTAAG
ApaI         2924  GGGCCC               GGGCC^C
BamHI         697  GGATCC               G^GATCC
BciVI         104  GGATAC               GTATCC(6/5)
BclI         3494  TGATCA               T^GATCA
BfrI           46  CTTAAG               C^TTAAG
BglI         4901  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1801  AGATCT               A^GATCT
BsaHI          94  GGCGTC               G[GA]^CG[CT]C
BsiEI        1374  CGATCG               CG[GA][CT]^CG
BspHI        5273  TCATGA               T^CATGA
BssSI        1887  CACGAG               CACGAG(-5/-1)
Bst1107I     5629  GTATAC               GTA^TAC
BstBI        4921  TTCGAA               TT^CGAA
BstEII        315  GGTGACC              G^GT[ACGT]ACC
BstPI         315  GGTGACC              G^GT[ACGT]ACC
BstZ17I      5629  GTATAC               GTA^TAC
CpoI           79  CGGACCG              CG^G[AT]CCG
DrdI         2381  GACCTCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       315  GGTGACC              G^GT[ACGT]ACC
FbaI         3494  TGATCA               T^GATCA
FseI          273  GGCCGGCC             GGCCGG^CC
HaeII        1721  GGCGCT               [GA]GCGC^[CT]
Hin1I          94  GGCGTC               G[GA]^CG[CT]C
HincII       5867  GTTAAC               GT[CT]^[GA]AC
HindII       5867  GTTAAC               GT[CT]^[GA]AC
HpaI         5867  GTTAAC               GTT^AAC
NaeI          274  GCCGGC               GCC^GGC
NdeI         5215  CATATG               CA^TATG
NgoMIV        274  GCCGGC               G^CCGGC
NspV         4921  TTCGAA               TT^CGAA
PshAI        4264  GACAAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         1374  CGATCG               CGAT^CG
RcaI         5273  TCATGA               T^CATGA
RsrII          79  CGGACCG              CG^G[AT]CCG
SapI         2489  GCTCTTC              GCTCTTC(1/4)
SbfI         4998  CCTGCAGG             CCTGCA^GG
SexAI         938  ACCTGGT              A^CC[AT]GGT
SfuI         4921  TTCGAA               TT^CGAA
SgrAI         263  CACCGGTG             C[GA]^CCGG[CT]G
SphI         1892  GCATGC               GCATG^C
Sse8387I     4998  CCTGCAGG             CCTGCA^GG
SwaI         4113  ATTTAAAT             ATTT^AAAT
XcmI         1385  CCAAGAATGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         1228  GTCTAC               GT^[AC][GT]AC
             5629  GTATAC              
AccII        4764  CGCG                 CG^CG
             4858  CGCG                
AclI          193  AACGTT               AA^CGTT
             3209  AACGTT              
AgeI          264  ACCGGT               A^CCGGT
             1192  ACCGGT              
Alw44I       1278  GTGCAC               G^TGCAC
             5016  GTGCAC              
ApaLI        1278  GTGCAC               G^TGCAC
             5016  GTGCAC              
BsaBI        1182  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2184  GATTTTAATC          
BsaWI         264  ACCGGT               [AT]^CCGG[AT]
             1192  ACCGGT              
BsmI         3024  GCATTC               GAATGC(1/-1)
             4087  GCATTC              
Bsp1407I     4548  TGTACA               T^GTACA
             5475  TGTACA              
BspMI          11  ACCTGC               ACCTGC(4/8)
             5001  GCAGGT              
BsrGI        4548  TGTACA               T^GTACA
             5475  TGTACA              
BstUI        4764  CGCG                 CG^CG
             4858  CGCG                
DraIII       1421  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3075  CACATGGTG           
EarI         2490  CTCTTC               CTCTTC(1/4)
             4126  CTCTTC              
EcoNI        4332  CCTATGATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4496  CCTTCTGAAGG         
EcoRI        1959  GAATTC               G^AATTC
             3170  GAATTC              
EcoRV        1186  GATATC               GAT^ATC
             1342  GATATC              
HgaI          511  GACGC                GACGC(5/10)
               95  GCGTC               
Ksp632I      2490  CTCTTC               CTCTTC(1/4)
             4126  CTCTTC              
MamI         1182  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2184  GATTTTAATC          
MvnI         4764  CGCG                 CG^CG
             4858  CGCG                
PinAI         264  ACCGGT               A^CCGGT
             1192  ACCGGT              
PpuMI         935  AGGACCT              [GA]G^G[AT]CC[CT]
             5482  AGGACCC             
Psp1406I      193  AACGTT               AA^CGTT
             3209  AACGTT              
SacI          130  GAGCTC               GAGCT^C
             1102  GAGCTC              
ScaI         1511  AGTACT               AGT^ACT
             5359  AGTACT              
SmaI         1542  CCCGGG               CCC^GGG
             1779  CCCGGG              
SpeI         3973  ACTAGT               A^CTAGT
             5169  ACTAGT              
SspBI        4548  TGTACA               T^GTACA
             5475  TGTACA              
SspI         4348  AATATT               AAT^ATT
             5242  AATATT              
XbaI         2391  TCTAGA               T^CTAGA
             5675  TCTAGA              
XmaCI        1542  CCCGGG               C^CCGGG
             1779  CCCGGG              
XmaI         1542  CCCGGG               C^CCGGG
             1779  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        Eco81I      EcoO109I    
EcoRII      EcoT14I     EcoT22I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PacI        PflMI       PleI        PshBI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII