##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI Asp700I Asp718I AviII BbeI
BbrPI BbvCI BcgI BciVI BsaAI
BsiWI BspDI BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI DrdI
EagI EclXI Eco52I EcoO65I FseI
FspI KasI KpnI KspI MluI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Aor51HI 3221 AGCGCT AGC^GCT
AseI 4571 ATTAAT AT^TAAT
AsnI 4571 ATTAAT AT^TAAT
AspI 1793 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 172 CTCGAG C^[CT]CG[GA]G
BclI 2893 TGATCA T^GATCA
BglI 2776 GCCTAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 3387 CGGTCG CG[GA][CT]^CG
BsoBI 172 CTCGAG C^[CT]CG[GA]G
BspHI 5576 TCATGA T^CATGA
BsrBI 3099 CCGCTC CCGCTC(-3/-3)
BssSI 5212 CTCGTG CACGAG(-5/-1)
CpoI 223 CGGACCG CG^G[AT]CCG
Eco47III 3221 AGCGCT AGC^GCT
EcoNI 1842 CCTTGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2893 TGATCA T^GATCA
HaeII 3221 AGCGCT [GA]GCGC^[CT]
MfeI 3030 CAATTG C^AATTG
MunI 3030 CAATTG C^AATTG
PaeR7I 172 CTCGAG C^TCGAG
PflFI 1793 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1127 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1914 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4571 ATTAAT AT^TAAT
RcaI 5576 TCATGA T^CATGA
RsrII 223 CGGACCG CG^G[AT]CCG
SalI 167 GTCGAC G^TCGAC
ScaI 1706 AGTACT AGT^ACT
SpeI 5477 ACTAGT A^CTAGT
Tth111I 1793 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1127 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3418 TCTAGA T^CTAGA
XcmI 2188 CCAAGGAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 172 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 143 GGCGTC G[GA]^CG[CT]C
1715 GGCGTC
AflII 305 CTTAAG C^TTAAG
5738 CTTAAG
AhdI 262 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACGAGCCGTC
AlwNI 8 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2505 CAGGGACTG
AspEI 262 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACGAGCCGTC
AvrII 209 CCTAGG C^CTAGG
5811 CCTAGG
BalI 948 TGGCCA TGG^CCA
5392 TGGCCA
BamHI 3819 GGATCC G^GATCC
3842 GGATCC
BanI 97 GGTGCC G^G[CT][GA]CC
923 GGTGCC
BfrI 305 CTTAAG C^TTAAG
5738 CTTAAG
BlnI 209 CCTAGG C^CTAGG
5811 CCTAGG
BsaBI 1489 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2362 GATTCCTATC
BsaHI 143 GGCGTC G[GA]^CG[CT]C
1715 GGCGTC
BseRI 633 GAGGAG GAGGAG(10/8)
3155 GAGGAG
Bst1107I 2845 GTATAC GTA^TAC
3498 GTATAC
BstXI 3774 CCAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5355 CCAAAGTGCTGG
BstZ17I 2845 GTATAC GTA^TAC
3498 GTATAC
DsaI 570 CCGTGG C^C[GA][CT]GG
2720 CCACGG
Eam1105I 262 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACGAGCCGTC
EcoRV 202 GATATC GAT^ATC
2952 GATATC
EcoT22I 3087 ATGCAT ATGCA^T
3507 ATGCAT
FauI 56 CCCGC CCCGC(4/6)
251 CCCGC
Hin1I 143 GGCGTC G[GA]^CG[CT]C
1715 GGCGTC
HpaI 2680 GTTAAC GTT^AAC
3565 GTTAAC
MamI 1489 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2362 GATTCCTATC
MluNI 948 TGGCCA TGG^CCA
5392 TGGCCA
MscI 948 TGGCCA TGG^CCA
5392 TGGCCA
NsiI 3087 ATGCAT ATGCA^T
3507 ATGCAT
SacI 863 GAGCTC GAGCT^C
4806 GAGCTC
SapI 1439 GAAGAGC GCTCTTC(1/4)
2285 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI Alw44I AlwI
AocI ApaLI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseAI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspEI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII