##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I ApaLI AscI
AseI AsnI Asp718I AvaI BalI
BbeI BcgI BglI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DrdI EaeI EagI EclXI
Eco52I FseI Hin1I KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI Sse8387I SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 5777 GTAGAC GT^[AC][GT]AC
AhdI 3896 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5014 AGCGCT AGC^GCT
AspEI 3896 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3052 TGCGCA TGC^GCA
AvrII 5565 CCTAGG C^CTAGG
BamHI 2444 GGATCC G^GATCC
BbrPI 3837 CACGTG CAC^GTG
BbvCI 1526 CCTCAGC CCTCAGC(-5/-2)
BglII 1598 AGATCT A^GATCT
BlnI 5565 CCTAGG C^CTAGG
BspLU11I 5339 ACATGT A^CATGT
BsrBI 2762 GAGCGG CCGCTC(-3/-3)
BsrDI 4025 CATTGC GCAATG(2/0)
BstAPI 4309 GCAATAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 736 GGTCACC G^GT[ACGT]ACC
BstPI 736 GGTCACC G^GT[ACGT]ACC
BstXI 478 CCATACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4171 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3534 CCACGG C^C[GA][CT]GG
Eam1105I 3896 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5014 AGCGCT AGC^GCT
EcoO65I 736 GGTCACC G^GT[ACGT]ACC
EcoRV 5497 GATATC GAT^ATC
FspI 3052 TGCGCA TGC^GCA
HaeII 5014 AGCGCT [GA]GCGC^[CT]
MfeI 3020 CAATTG C^AATTG
MunI 3020 CAATTG C^AATTG
NheI 3083 GCTAGC G^CTAGC
PflMI 497 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3837 CACGTG CAC^GTG
PmeI 5718 GTTTAAAC GTTT^AAAC
PmlI 3837 CACGTG CAC^GTG
PvuII 5286 CAGCTG CAG^CTG
SacI 901 GAGCTC GAGCT^C
ScaI 4981 AGTACT AGT^ACT
SgrAI 3872 CACCGGCG C[GA]^CCGG[CT]G
SpeI 772 ACTAGT A^CTAGT
StuI 3117 AGGCCT AGG^CCT
Van91I 497 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 5404 CTTAAG C^TTAAG
5707 CTTAAG
AocI 2043 CCTCAGG CC^T[ACGT]AGG
2439 CCTCAGG
ApaI 2114 GGGCCC GGGCC^C
4065 GGGCCC
AspI 943 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACCGGGTC
BanI 985 GGTGCC G^G[CT][GA]CC
1333 GGCACC
BciVI 2468 GTATCC GTATCC(6/5)
2586 GTATCC
BcnI 1806 CCGGG CC^[GC]GG
2818 CCGGG
BfrI 5404 CTTAAG C^TTAAG
5707 CTTAAG
BlpI 2353 GCTGAGC GC^T[ACGT]AGC
4349 GCTCAGC
Bpu1102I 2353 GCTGAGC GC^T[ACGT]AGC
4349 GCTCAGC
BsaAI 3837 CACGTG [CT]AC^GT[GA]
5610 TACGTG
BsaI 1802 GAGACC GGTCTC(1/5)
1063 GGTCTC
Bsp1407I 5275 TGTACA T^GTACA
5342 TGTACA
BspHI 169 TCATGA T^CATGA
5491 TCATGA
BsrFI 3873 ACCGGC [GA]^CCGG[CT]
5616 ACCGGC
BsrGI 5275 TGTACA T^GTACA
5342 TGTACA
Bsu36I 2043 CCTCAGG CC^T[ACGT]AGG
2439 CCTCAGG
CelII 2353 GCTGAGC GC^T[ACGT]AGC
4349 GCTCAGC
Cfr10I 3873 ACCGGC [GA]^CCGG[CT]
5616 ACCGGC
Eco81I 2043 CCTCAGG CC^T[ACGT]AGG
2439 CCTCAGG
EcoT22I 6 ATGCAT ATGCA^T
215 ATGCAT
FauI 1123 CCCGC CCCGC(4/6)
2764 GCGGG
HpaI 428 GTTAAC GTT^AAC
2810 GTTAAC
MspA1I 2385 CCGCTG C[AC]G^C[GT]G
5286 CAGCTG
NciI 1806 CCGGG CC^[GC]GG
2818 CCGGG
NsiI 6 ATGCAT ATGCA^T
215 ATGCAT
PflFI 943 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACCGGGTC
PpuMI 2509 AGGTCCT [GA]G^G[AT]CC[CT]
3636 GGGACCT
PstI 2684 CTGCAG CTGCA^G
3787 CTGCAG
RcaI 169 TCATGA T^CATGA
5491 TCATGA
SapI 3285 GAAGAGC GCTCTTC(1/4)
4387 GAAGAGC
SphI 574 GCATGC GCATG^C
1796 GCATGC
SspBI 5275 TGTACA T^GTACA
5342 TGTACA
SspI 131 AATATT AAT^ATT
5323 AATATT
Tth111I 943 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACCGGGTC
XbaI 1472 TCTAGA T^CTAGA
3331 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI