##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII BalI
BbeI BbrPI BcnI BglI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
BstUI BstXI CelII CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 2758 CTTAAG C^TTAAG
AflIII 2161 ACATGT A^C[GA][CT]GT
Alw44I 2180 GTGCAC G^TGCAC
ApaLI 2180 GTGCAC G^TGCAC
AvrII 5839 CCTAGG C^CTAGG
BamHI 1614 GGATCC G^GATCC
BbvCI 4879 GCTGAGG CCTCAGC(-5/-2)
BfrI 2758 CTTAAG C^TTAAG
BlnI 5839 CCTAGG C^CTAGG
BsaAI 2063 TACGTG [CT]AC^GT[GA]
BsaWI 3167 TCCGGT [AT]^CCGG[AT]
BsmI 3630 GAATGC GAATGC(1/-1)
BspHI 4066 TCATGA T^CATGA
BspLU11I 2161 ACATGT A^CATGT
BstAPI 2173 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 131 TTCGAA TT^CGAA
BstEII 716 GGTCACC G^GT[ACGT]ACC
BstPI 716 GGTCACC G^GT[ACGT]ACC
EaeI 1190 CGGCCA [CT]^GGCC[GA]
EcoO65I 716 GGTCACC G^GT[ACGT]ACC
EcoT22I 4010 ATGCAT ATGCA^T
HapII 3168 CCGG C^CGG
HpaII 3168 CCGG C^CGG
MfeI 4632 CAATTG C^AATTG
MspI 3168 CCGG C^CGG
MunI 4632 CAATTG C^AATTG
NsiI 4010 ATGCAT ATGCA^T
NspV 131 TTCGAA TT^CGAA
RcaI 4066 TCATGA T^CATGA
SacI 769 GAGCTC GAGCT^C
SapI 3282 GCTCTTC GCTCTTC(1/4)
SfuI 131 TTCGAA TT^CGAA
SspI 4118 AATATT AAT^ATT
SwaI 5132 ATTTAAAT ATTT^AAAT
XbaI 5200 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AclI 410 AACGTT AA^CGTT
4551 AACGTT
AspI 2790 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACCATGTC
BanII 769 GAGCTC G[GA]GC[CT]^C
1125 GGGCTC
BcgI 1768 CGAGTTTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3369 CGAGCAGTGTGC
BciVI 4576 GGATAC GTATCC(6/5)
2851 GTATCC
BsrDI 1026 GCAATG GCAATG(2/0)
1165 GCAATG
Bst1107I 4285 GTATAC GTA^TAC
5387 GTATAC
BstZ17I 4285 GTATAC GTA^TAC
5387 GTATAC
DraIII 1037 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3359 CACTGAGTG
DsaI 878 CCATGG C^C[GA][CT]GG
5623 CCATGG
HincII 2796 GTCAAC GT[CT]^[GA]AC
2904 GTCAAC
HindII 2796 GTCAAC GT[CT]^[GA]AC
2904 GTCAAC
HindIII 2930 AAGCTT A^AGCTT
4022 AAGCTT
NcoI 878 CCATGG C^CATGG
5623 CCATGG
PflFI 2790 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACCATGTC
Psp1406I 410 AACGTT AA^CGTT
4551 AACGTT
SexAI 216 ACCTGGT A^CC[AT]GGT
712 ACCTGGT
Tth111I 2790 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2867 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BbsI BbvI BclI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI