##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        ApaI        AscI        
Asp718I     AspEI       AviII       BamHI       BanII       
BbeI        BbvCI       BcgI        BfrI        BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspMI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        Eam1105I    EcoNI       EcoRV       EcoT22I     
FseI        FspI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcyI           89  GACGCC               G[GA]^CG[CT]C
Alw44I        439  GTGCAC               G^TGCAC
AocI         3320  CCTAAGG              CC^T[ACGT]AGG
ApaLI         439  GTGCAC               G^TGCAC
AseI         3027  ATTAAT               AT^TAAT
AsnI         3027  ATTAAT               AT^TAAT
AspHI         439  GTGCAC               G[AT]GC[AT]^C
AspI           89  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          461  CTCGGG               C^[CT]CG[GA]G
BalI          387  TGGCCA               TGG^CCA
BbrPI         577  CACGTG               CAC^GTG
BciVI        4465  GTATCC               GTATCC(6/5)
BlpI           64  GCTGAGC              GC^T[ACGT]AGC
BpmI           34  CTCCAG               CTGGAG(16/14)
Bpu1102I       64  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1073  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          89  GACGCC               G[GA]^CG[CT]C
BsaI         1733  GAGACC               GGTCTC(1/5)
BsiHKAI       439  GTGCAC               G[AT]GC[AT]^C
BsmBI         787  CGTCTC               CGTCTC(1/5)
BsoBI         461  CTCGGG               C^[CT]CG[GA]G
BspLU11I     1128  ACATGT               A^CATGT
BsrBI         448  GAGCGG               CCGCTC(-3/-3)
BsrFI         410  ACCGGC               [GA]^CCGG[CT]
BssSI        1331  CTCGTG               CACGAG(-5/-1)
BstAPI        957  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1159  GGTGACC              G^GT[ACGT]ACC
BstPI        1159  GGTGACC              G^GT[ACGT]ACC
Bsu36I       3320  CCTAAGG              CC^T[ACGT]AGG
CelII          64  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        410  ACCGGC               [GA]^CCGG[CT]
DraIII        391  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       3320  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      1159  GGTGACC              G^GT[ACGT]ACC
Hin1I          89  GACGCC               G[GA]^CG[CT]C
HincII       2001  GTTAAC               GT[CT]^[GA]AC
HindII       2001  GTTAAC               GT[CT]^[GA]AC
HpaI         2001  GTTAAC               GTT^AAC
MamI         1073  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         387  TGGCCA               TGG^CCA
MscI          387  TGGCCA               TGG^CCA
NheI         1527  GCTAGC               G^CTAGC
PflFI          89  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         577  CACGTG               CAC^GTG
PmlI          577  CACGTG               CAC^GTG
PpuMI         636  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         179  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3027  ATTAAT               AT^TAAT
PvuII        3220  CAGCTG               CAG^CTG
SpeI         2246  ACTAGT               A^CTAGT
StuI         3429  AGGCCT               AGG^CCT
SwaI         3629  ATTTAAAT             ATTT^AAAT
Tth111I        89  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Aor51HI       999  AGCGCT               AGC^GCT
             1323  AGCGCT              
Asp700I      1473  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3968  GAAAGAATTC          
AvaII         637  GGACC                G^G[AT]CC
             1170  GGACC               
BanI           81  GGCACC               G^G[CT][GA]CC
             4455  GGTGCC              
BglI           92  GCCGTCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1624  GCCTAGGGGGC         
BglII        1188  AGATCT               A^GATCT
             1803  AGATCT              
BsaAI         577  CACGTG               [CT]AC^GT[GA]
             2774  CACGTA              
BseRI         849  CTCCTC               GAGGAG(10/8)
             2430  GAGGAG              
Bsp1407I     3693  TGTACA               T^GTACA
             4546  TGTACA              
BsrDI        1141  CATTGC               GCAATG(2/0)
             2039  CATTGC              
BsrGI        3693  TGTACA               T^GTACA
             4546  TGTACA              
DrdI         2652  GACAATAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3462  GACAGGCTAGTC        
EagI           21  CGGCCG               C^GGCCG
               99  CGGCCG              
EclXI          21  CGGCCG               C^GGCCG
               99  CGGCCG              
Eco47III      999  AGCGCT               AGC^GCT
             1323  AGCGCT              
Eco52I         21  CGGCCG               C^GGCCG
               99  CGGCCG              
EcoRI        3087  GAATTC               G^AATTC
             3972  GAATTC              
HaeII         999  AGCGCT               [GA]GCGC^[CT]
             1323  AGCGCT              
MspA1I        647  CAGCGG               C[AC]G^C[GT]G
             3220  CAGCTG              
NdeI         2668  CATATG               CA^TATG
             3484  CATATG              
PflMI         208  CCACACAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              390  CCACAGAGTGG         
ScaI         2620  AGTACT               AGT^ACT
             4088  AGTACT              
SmlI         1003  CTCAAG               C^T[CT][GA]AG
             1700  CTTGAG              
SspBI        3693  TGTACA               T^GTACA
             4546  TGTACA              
Van91I        208  CCACACAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              390  CCACAGAGTGG         
XbaI         2925  TCTAGA               T^CTAGA
             3940  TCTAGA              
XcmI         1146  CCAGGGGACTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1638  CCAGGGTTCTTGTGG     
XmnI         1473  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3968  GAAAGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AvrII       
BbsI        BbvI        BclI        BcnI        BfaI        
BlnI        BmyI        BpuAI       BsaJI       BsgI        
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspHI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII