################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI ApaI AscI Asp718I AspEI AviII BamHI BanII BbeI BbvCI BcgI BfrI BsaWI BseAI BsiWI BspDI BspEI BspMI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I EcoNI EcoRV EcoT22I FseI FspI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 89 GACGCC G[GA]^CG[CT]C Alw44I 439 GTGCAC G^TGCAC AocI 3320 CCTAAGG CC^T[ACGT]AGG ApaLI 439 GTGCAC G^TGCAC AseI 3027 ATTAAT AT^TAAT AsnI 3027 ATTAAT AT^TAAT AspHI 439 GTGCAC G[AT]GC[AT]^C AspI 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 461 CTCGGG C^[CT]CG[GA]G BalI 387 TGGCCA TGG^CCA BbrPI 577 CACGTG CAC^GTG BciVI 4465 GTATCC GTATCC(6/5) BlpI 64 GCTGAGC GC^T[ACGT]AGC BpmI 34 CTCCAG CTGGAG(16/14) Bpu1102I 64 GCTGAGC GC^T[ACGT]AGC BsaBI 1073 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 89 GACGCC G[GA]^CG[CT]C BsaI 1733 GAGACC GGTCTC(1/5) BsiHKAI 439 GTGCAC G[AT]GC[AT]^C BsmBI 787 CGTCTC CGTCTC(1/5) BsoBI 461 CTCGGG C^[CT]CG[GA]G BspLU11I 1128 ACATGT A^CATGT BsrBI 448 GAGCGG CCGCTC(-3/-3) BsrFI 410 ACCGGC [GA]^CCGG[CT] BssSI 1331 CTCGTG CACGAG(-5/-1) BstAPI 957 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1159 GGTGACC G^GT[ACGT]ACC BstPI 1159 GGTGACC G^GT[ACGT]ACC Bsu36I 3320 CCTAAGG CC^T[ACGT]AGG CelII 64 GCTGAGC GC^T[ACGT]AGC Cfr10I 410 ACCGGC [GA]^CCGG[CT] DraIII 391 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 3320 CCTAAGG CC^T[ACGT]AGG EcoO65I 1159 GGTGACC G^GT[ACGT]ACC Hin1I 89 GACGCC G[GA]^CG[CT]C HincII 2001 GTTAAC GT[CT]^[GA]AC HindII 2001 GTTAAC GT[CT]^[GA]AC HpaI 2001 GTTAAC GTT^AAC MamI 1073 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 387 TGGCCA TGG^CCA MscI 387 TGGCCA TGG^CCA NheI 1527 GCTAGC G^CTAGC PflFI 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 577 CACGTG CAC^GTG PmlI 577 CACGTG CAC^GTG PpuMI 636 GGGACCC [GA]G^G[AT]CC[CT] PshAI 179 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3027 ATTAAT AT^TAAT PvuII 3220 CAGCTG CAG^CTG SpeI 2246 ACTAGT A^CTAGT StuI 3429 AGGCCT AGG^CCT SwaI 3629 ATTTAAAT ATTT^AAAT Tth111I 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Aor51HI 999 AGCGCT AGC^GCT 1323 AGCGCT Asp700I 1473 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3968 GAAAGAATTC AvaII 637 GGACC G^G[AT]CC 1170 GGACC BanI 81 GGCACC G^G[CT][GA]CC 4455 GGTGCC BglI 92 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1624 GCCTAGGGGGC BglII 1188 AGATCT A^GATCT 1803 AGATCT BsaAI 577 CACGTG [CT]AC^GT[GA] 2774 CACGTA BseRI 849 CTCCTC GAGGAG(10/8) 2430 GAGGAG Bsp1407I 3693 TGTACA T^GTACA 4546 TGTACA BsrDI 1141 CATTGC GCAATG(2/0) 2039 CATTGC BsrGI 3693 TGTACA T^GTACA 4546 TGTACA DrdI 2652 GACAATAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3462 GACAGGCTAGTC EagI 21 CGGCCG C^GGCCG 99 CGGCCG EclXI 21 CGGCCG C^GGCCG 99 CGGCCG Eco47III 999 AGCGCT AGC^GCT 1323 AGCGCT Eco52I 21 CGGCCG C^GGCCG 99 CGGCCG EcoRI 3087 GAATTC G^AATTC 3972 GAATTC HaeII 999 AGCGCT [GA]GCGC^[CT] 1323 AGCGCT MspA1I 647 CAGCGG C[AC]G^C[GT]G 3220 CAGCTG NdeI 2668 CATATG CA^TATG 3484 CATATG PflMI 208 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACAGAGTGG ScaI 2620 AGTACT AGT^ACT 4088 AGTACT SmlI 1003 CTCAAG C^T[CT][GA]AG 1700 CTTGAG SspBI 3693 TGTACA T^GTACA 4546 TGTACA Van91I 208 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACAGAGTGG XbaI 2925 TCTAGA T^CTAGA 3940 TCTAGA XcmI 1146 CCAGGGGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1638 CCAGGGTTCTTGTGG XmnI 1473 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3968 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AvrII BbsI BbvI BclI BcnI BfaI BlnI BmyI BpuAI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII