##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
StuI SwaI XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 33 CCGC CCGC(-3/-1)
AflII 1743 CTTAAG C^TTAAG
Alw44I 418 GTGCAC G^TGCAC
AlwNI 2335 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 418 GTGCAC G^TGCAC
AspI 879 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 627 CTCGAG C^[CT]CG[GA]G
AvaII 2330 GGTCC G^G[AT]CC
BclI 380 TGATCA T^GATCA
BfrI 1743 CTTAAG C^TTAAG
BglI 2372 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 829 AGATCT A^GATCT
BsaBI 1350 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2412 CTGCAC GTGCAG(16/14)
BsoBI 627 CTCGAG C^[CT]CG[GA]G
BspMI 529 GCAGGT ACCTGC(4/8)
Bst1107I 2808 GTATAC GTA^TAC
BstYI 829 AGATCT [GA]^GATC[CT]
BstZ17I 2808 GTATAC GTA^TAC
DsaI 83 CCATGG C^C[GA][CT]GG
EcoT22I 2681 ATGCAT ATGCA^T
FbaI 380 TGATCA T^GATCA
HgaI 65 GCGTC GACGC(5/10)
HincII 760 GTCAAC GT[CT]^[GA]AC
HindII 760 GTCAAC GT[CT]^[GA]AC
HindIII 2953 AAGCTT A^AGCTT
MamI 1350 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 455 CAATTG C^AATTG
MflI 829 AGATCT [GA]^GATC[CT]
MunI 455 CAATTG C^AATTG
NcoI 83 CCATGG C^CATGG
NdeI 2319 CATATG CA^TATG
NsiI 2681 ATGCAT ATGCA^T
PaeR7I 627 CTCGAG C^TCGAG
PflFI 879 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 826 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1089 GACTC GAGTC(4/5)
PshAI 1317 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1319 CAGCTG CAG^CTG
SphI 996 GCATGC GCATG^C
TaqI 628 TCGA T^CGA
Tth111I 879 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 628 TCGA T^CGA
Van91I 826 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 627 CTCGAG C^TCGAG
XhoII 829 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 403 GATCC GGATC(4/5)
1144 GGATC
AseI 1113 ATTAAT AT^TAAT
2992 ATTAAT
AsnI 1113 ATTAAT AT^TAAT
2992 ATTAAT
AspHI 78 GTGCTC G[AT]GC[AT]^C
418 GTGCAC
BalI 1654 TGGCCA TGG^CCA
2627 TGGCCA
BanI 532 GGTGCC G^G[CT][GA]CC
1383 GGCACC
BanII 2243 GAGCCC G[GA]GC[CT]^C
2370 GAGCCC
BbsI 2570 GAAGAC GAAGAC(2/6)
846 GTCTTC
BbvCI 2222 GCTGAGG CCTCAGC(-5/-2)
2349 GCTGAGG
BpmI 1523 CTCCAG CTGGAG(16/14)
1150 CTGGAG
BpuAI 2570 GAAGAC GAAGAC(2/6)
846 GTCTTC
BseRI 769 CTCCTC GAGGAG(10/8)
2343 GAGGAG
BsiHKAI 78 GTGCTC G[AT]GC[AT]^C
418 GTGCAC
BsmAI 1422 GAGAC GTCTC(1/5)
14 GTCTC
BsmI 475 GCATTC GAATGC(1/-1)
2639 GCATTC
BspHI 1256 TCATGA T^CATGA
2059 TCATGA
BssSI 1295 CTCGTG CACGAG(-5/-1)
1378 CTCGTG
BstAPI 451 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2381 GCAGAGGTTGC
BstXI 1525 CCAGTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2207 CCAGCACTTTGG
Cfr13I 2177 GGGCC G^G[ACGT]CC
2330 GGTCC
EaeI 1654 TGGCCA [CT]^GGCC[GA]
2627 TGGCCA
EcoRI 2251 GAATTC G^AATTC
3100 GAATTC
EcoT14I 83 CCATGG C^C[AT][AT]GG
2398 CCAAGG
MluNI 1654 TGGCCA TGG^CCA
2627 TGGCCA
MscI 1654 TGGCCA TGG^CCA
2627 TGGCCA
MspA1I 33 CCGCTG C[AC]G^C[GT]G
1319 CAGCTG
PshBI 1113 ATTAAT AT^TAAT
2992 ATTAAT
PstI 413 CTGCAG CTGCA^G
449 CTGCAG
RcaI 1256 TCATGA T^CATGA
2059 TCATGA
Sau96I 2177 GGGCC G^G[ACGT]CC
2330 GGTCC
SpeI 558 ACTAGT A^CTAGT
2939 ACTAGT
SspI 1792 AATATT AAT^ATT
2985 AATATT
StyI 83 CCATGG C^C[AT][AT]GG
2398 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
BbvI BciVI BfaI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI