##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AocI Aor51HI AscI AviII AvrII
BcgI BfrI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII Bsu36I ClaI DraIII EagI
EclXI Eco47III Eco52I Eco81I FseI
FspI MluI MroI NcoI NotI
NruI PacI PflMI PinAI PmeI
PshAI Psp1406I PvuI SalI SbfI
ScaI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
Acc65I 1827 GGTACC G^GTACC
AhdI 85 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5663 GTGCAC G^TGCAC
ApaI 4375 GGGCCC GGGCC^C
ApaLI 5663 GTGCAC G^TGCAC
Asp718I 1827 GGTACC G^GTACC
AspEI 85 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1532 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4228 CTCGAG C^[CT]CG[GA]G
BalI 1097 TGGCCA TGG^CCA
BamHI 4304 GGATCC G^GATCC
BbrPI 4315 CACGTG CAC^GTG
BciVI 2313 GGATAC GTATCC(6/5)
BglI 5208 GCCCACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1676 AGATCT A^GATCT
BlpI 3961 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3961 GCTGAGC GC^T[ACGT]AGC
BsaBI 2741 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4103 GAATGC GAATGC(1/-1)
BsoBI 4228 CTCGAG C^[CT]CG[GA]G
Bsp1407I 5901 TGTACA T^GTACA
BspHI 3726 TCATGA T^CATGA
BsrGI 5901 TGTACA T^GTACA
BssSI 5660 CTCGTG CACGAG(-5/-1)
Bst1107I 776 GTATAC GTA^TAC
BstBI 5057 TTCGAA TT^CGAA
BstEII 3111 GGTGACC G^GT[ACGT]ACC
BstPI 3111 GGTGACC G^GT[ACGT]ACC
BstZ17I 776 GTATAC GTA^TAC
CelII 3961 GCTGAGC GC^T[ACGT]AGC
CpoI 4289 CGGACCG CG^G[AT]CCG
DrdI 5729 GACAATATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 85 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3111 GGTGACC G^GT[ACGT]ACC
EcoRI 1556 GAATTC G^AATTC
EcoRV 947 GATATC GAT^ATC
EcoT22I 5871 ATGCAT ATGCA^T
HpaI 3847 GTTAAC GTT^AAC
KpnI 1827 GGTACC GGTAC^C
KspI 4090 CCGCGG CCGC^GG
MamI 2741 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3698 CAATTG C^AATTG
MluNI 1097 TGGCCA TGG^CCA
MscI 1097 TGGCCA TGG^CCA
MunI 3698 CAATTG C^AATTG
NaeI 327 GCCGGC GCC^GGC
NgoMIV 327 GCCGGC G^CCGGC
NsiI 5871 ATGCAT ATGCA^T
NspV 5057 TTCGAA TT^CGAA
PaeR7I 4228 CTCGAG C^TCGAG
PflFI 1532 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4315 CACGTG CAC^GTG
PmlI 4315 CACGTG CAC^GTG
RcaI 3726 TCATGA T^CATGA
RsrII 4289 CGGACCG CG^G[AT]CCG
SacII 4090 CCGCGG CCGC^GG
SapI 3745 GAAGAGC GCTCTTC(1/4)
SexAI 2463 ACCTGGT A^CC[AT]GGT
SfuI 5057 TTCGAA TT^CGAA
SphI 527 GCATGC GCATG^C
SspBI 5901 TGTACA T^GTACA
SwaI 5764 ATTTAAAT ATTT^AAAT
Tth111I 1532 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3649 CCAAGTCAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4228 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 206 ACATGT A^C[GA][CT]GT
5404 ACATGT
Asp700I 2260 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4509 GAAACTTTTC
BbeI 125 GGCGCC GGCGC^C
4790 GGCGCC
BclI 291 TGATCA T^GATCA
5687 TGATCA
BsaAI 299 CACGTA [CT]AC^GT[GA]
4315 CACGTG
BspLU11I 206 ACATGT A^CATGT
5404 ACATGT
BspMI 3453 ACCTGC ACCTGC(4/8)
5144 ACCTGC
BstAPI 3271 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5335 GCAATCTATGC
DraI 4068 TTTAAA TTT^AAA
5765 TTTAAA
DsaI 4090 CCGCGG C^C[GA][CT]GG
5521 CCGTGG
EaeI 1097 TGGCCA [CT]^GGCC[GA]
3956 TGGCCG
EcoT14I 3530 CCAAGG C^C[AT][AT]GG
5431 CCAAGG
FbaI 291 TGATCA T^GATCA
5687 TGATCA
HaeII 125 GGCGCC [GA]GCGC^[CT]
4790 GGCGCC
HincII 3653 GTCAAC GT[CT]^[GA]AC
3847 GTTAAC
HindII 3653 GTCAAC GT[CT]^[GA]AC
3847 GTTAAC
KasI 125 GGCGCC G^GCGCC
4790 GGCGCC
NarI 125 GGCGCC GG^CGCC
4790 GGCGCC
PpuMI 865 GGGACCT [GA]G^G[AT]CC[CT]
5111 AGGTCCC
SacI 3517 GAGCTC GAGCT^C
3859 GAGCTC
SfoI 125 GGCGCC GGC^GCC
4790 GGCGCC
StuI 2570 AGGCCT AGG^CCT
4329 AGGCCT
StyI 3530 CCAAGG C^C[AT][AT]GG
5431 CCAAGG
XmnI 2260 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4509 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NheI NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII