##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BlnI BsaBI BsiEI BsiWI
BspDI BspHI BstAPI BstBI ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI HindIII KpnI
MamI MluI NaeI NdeI NgoMIV
NheI NotI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1059 GACGTC GACGT^C
AccIII 1636 TCCGGA T^CCGGA
AflII 3517 CTTAAG C^TTAAG
Aor51HI 775 AGCGCT AGC^GCT
BalI 4413 TGGCCA TGG^CCA
BbeI 142 GGCGCC GGCGC^C
BbvCI 2432 CCTCAGC CCTCAGC(-5/-2)
BciVI 2627 GTATCC GTATCC(6/5)
BclI 2254 TGATCA T^GATCA
BfrI 3517 CTTAAG C^TTAAG
BsaWI 1636 TCCGGA [AT]^CCGG[AT]
BseAI 1636 TCCGGA T^CCGGA
BsmBI 5345 CGTCTC CGTCTC(1/5)
Bsp1407I 4780 TGTACA T^GTACA
BspEI 1636 TCCGGA T^CCGGA
BspLU11I 2943 ACATGT A^CATGT
BsrDI 6116 GCAATG GCAATG(2/0)
BsrGI 4780 TGTACA T^GTACA
BssSI 738 CACGAG CACGAG(-5/-1)
DraIII 1296 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2108 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 775 AGCGCT AGC^GCT
EcoRI 2460 GAATTC G^AATTC
EcoT22I 1999 ATGCAT ATGCA^T
FbaI 2254 TGATCA T^GATCA
KasI 142 GGCGCC G^GCGCC
KspI 701 CCGCGG CCGC^GG
MfeI 2818 CAATTG C^AATTG
MluNI 4413 TGGCCA TGG^CCA
MroI 1636 TCCGGA T^CCGGA
MscI 4413 TGGCCA TGG^CCA
MunI 2818 CAATTG C^AATTG
NarI 142 GGCGCC GG^CGCC
NruI 2 TCGCGA TCG^CGA
NsiI 1999 ATGCAT ATGCA^T
PaeR7I 152 CTCGAG C^TCGAG
PflMI 1205 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2069 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 444 GAGCTC GAGCT^C
SacII 701 CCGCGG CCGC^GG
SexAI 1360 ACCAGGT A^CC[AT]GGT
SfoI 142 GGCGCC GGC^GCC
SspBI 4780 TGTACA T^GTACA
StuI 4693 AGGCCT AGG^CCT
Van91I 1205 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5290 TCTAGA T^CTAGA
XhoI 152 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 2191 CCTCAGG CC^T[ACGT]AGG
5124 CCTGAGG
ApaI 578 GGGCCC GGGCC^C
1582 GGGCCC
AspI 1076 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4962 GACTGAGTC
BcgI 154 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1520 CGAGCTGCTTGC
BglI 4695 GCCTTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5799 GCCTTTTTGGC
BlpI 3223 GCTGAGC GC^T[ACGT]AGC
4923 GCTTAGC
Bpu1102I 3223 GCTGAGC GC^T[ACGT]AGC
4923 GCTTAGC
BsmI 1373 GAATGC GAATGC(1/-1)
2219 GCATTC
BsrFI 1492 ACCGGC [GA]^CCGG[CT]
4498 GCCGGT
BssHII 22 GCGCGC G^CGCGC
586 GCGCGC
Bst1107I 4008 GTATAC GTA^TAC
4652 GTATAC
BstEII 1511 GGTGACC G^GT[ACGT]ACC
3801 GGTCACC
BstPI 1511 GGTGACC G^GT[ACGT]ACC
3801 GGTCACC
BstZ17I 4008 GTATAC GTA^TAC
4652 GTATAC
Bsu36I 2191 CCTCAGG CC^T[ACGT]AGG
5124 CCTGAGG
CelII 3223 GCTGAGC GC^T[ACGT]AGC
4923 GCTTAGC
Cfr10I 1492 ACCGGC [GA]^CCGG[CT]
4498 GCCGGT
Eco81I 2191 CCTCAGG CC^T[ACGT]AGG
5124 CCTGAGG
EcoO65I 1511 GGTGACC G^GT[ACGT]ACC
3801 GGTCACC
HincII 1752 GTTAAC GT[CT]^[GA]AC
3032 GTTAAC
HindII 1752 GTTAAC GT[CT]^[GA]AC
3032 GTTAAC
HpaI 1752 GTTAAC GTT^AAC
3032 GTTAAC
PflFI 1076 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4962 GACTGAGTC
SmlI 152 CTCGAG C^T[CT][GA]AG
3517 CTTAAG
SphI 1419 GCATGC GCATG^C
3137 GCATGC
SspI 1866 AATATT AAT^ATT
6108 AATATT
Tth111I 1076 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4962 GACTGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI