##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AflII AgeI AscI
AspI AviII AvrII BbeI BfrI
BlnI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
KasI MluI MroI NarI NotI
NruI NsiI NspI NspV PflFI
PinAI PmeI PshAI PvuI RsrII
SalI SapI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1833 GACGTC GACGT^C
Acc65I 3859 GGTACC G^GTACC
AclI 108 AACGTT AA^CGTT
AhdI 1452 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2489 AGCGCT AGC^GCT
AseI 4807 ATTAAT AT^TAAT
AsnI 4807 ATTAAT AT^TAAT
Asp718I 3859 GGTACC G^GTACC
AspEI 1452 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3662 GGATCC G^GATCC
BciVI 4465 GTATCC GTATCC(6/5)
BclI 4526 TGATCA T^GATCA
BglII 1601 AGATCT A^GATCT
BsaBI 3152 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4357 CGACCG CG[GA][CT]^CG
BspHI 3342 TCATGA T^CATGA
BssSI 876 CACGAG CACGAG(-5/-1)
BstXI 1664 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3699 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1452 GACCACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2489 AGCGCT AGC^GCT
EcoRV 3156 GATATC GAT^ATC
FbaI 4526 TGATCA T^GATCA
HpaI 3584 GTTAAC GTT^AAC
KpnI 3859 GGTACC GGTAC^C
KspI 3772 CCGCGG CCGC^GG
MamI 3152 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1737 CAATTG C^AATTG
MunI 1737 CAATTG C^AATTG
NaeI 1190 GCCGGC GCC^GGC
NdeI 4841 CATATG CA^TATG
NgoMIV 1190 GCCGGC G^CCGGC
NheI 2646 GCTAGC G^CTAGC
PacI 4804 TTAATTAA TTAAT^TAA
PshBI 4807 ATTAAT AT^TAAT
Psp1406I 108 AACGTT AA^CGTT
PvuII 4552 CAGCTG CAG^CTG
RcaI 3342 TCATGA T^CATGA
SacII 3772 CCGCGG CCGC^GG
SexAI 2860 ACCTGGT A^CC[AT]GGT
SfiI 1206 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 1048 GTGCAC G^TGCAC
3937 GTGCAC
ApaLI 1048 GTGCAC G^TGCAC
3937 GTGCAC
Asp700I 227 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3504 GAATCGGTTC
BbrPI 521 CACGTG CAC^GTG
4397 CACGTG
BcgI 2207 CGACCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1861 GCAGCGCGTTCG
BlpI 923 GCTCAGC GC^T[ACGT]AGC
1236 GCTGAGC
Bpu1102I 923 GCTCAGC GC^T[ACGT]AGC
1236 GCTGAGC
BsaWI 2013 ACCGGA [AT]^CCGG[AT]
4888 TCCGGT
BsrDI 3069 CATTGC GCAATG(2/0)
435 GCAATG
BstAPI 476 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4315 GCACGCACTGC
BstYI 1601 AGATCT [GA]^GATC[CT]
3662 GGATCC
CelII 923 GCTCAGC GC^T[ACGT]AGC
1236 GCTGAGC
DrdI 2976 GACCGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3868 GACGGCCCCGTC
HincII 1962 GTTGAC GT[CT]^[GA]AC
3584 GTTAAC
HindII 1962 GTTGAC GT[CT]^[GA]AC
3584 GTTAAC
HindIII 45 AAGCTT A^AGCTT
501 AAGCTT
MflI 1601 AGATCT [GA]^GATC[CT]
3662 GGATCC
NcoI 1271 CCATGG C^CATGG
3863 CCATGG
PaeR7I 360 CTCGAG C^TCGAG
1282 CTCGAG
PflMI 959 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2252 CCAGCGTATGG
PmaCI 521 CACGTG CAC^GTG
4397 CACGTG
PmlI 521 CACGTG CAC^GTG
4397 CACGTG
SacI 921 GAGCTC GAGCT^C
1653 GAGCTC
SbfI 1445 CCTGCAGG CCTGCA^GG
1847 CCTGCAGG
Sse8387I 1445 CCTGCAGG CCTGCA^GG
1847 CCTGCAGG
StuI 388 AGGCCT AGG^CCT
1068 AGGCCT
Van91I 959 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2252 CCAGCGTATGG
XhoI 360 CTCGAG C^TCGAG
1282 CTCGAG
XhoII 1601 AGATCT [GA]^GATC[CT]
3662 GGATCC
XmnI 227 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3504 GAATCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI