##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        Aor51HI     ApaI        AscI        Asp718I     
AviII       AvrII       BbeI        BbrPI       BcgI        
BclI        BglI        BlnI        BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspMI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    FbaI        
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NgoMIV      NotI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        Sse8387I    XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1815  GTAGAC               GT^[AC][GT]AC
AflIII       1698  ACATGT               A^C[GA][CT]GT
AhdI          100  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4477  GTGCAC               G^TGCAC
AlwNI        1902  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        4477  GTGCAC               G^TGCAC
AspEI         100  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        4781  GGACC                G^G[AT]CC
BanII        3063  GGGCTC               G[GA]GC[CT]^C
BbsI         1188  GAAGAC               GAAGAC(2/6)
BbvCI        1937  CCTCAGC              CCTCAGC(-5/-2)
BcnI         3219  CCGGG                CC^[GC]GG
BglII        1538  AGATCT               A^GATCT
BlpI         2157  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2157  GCTTAGC              GC^T[ACGT]AGC
BpuAI        1188  GAAGAC               GAAGAC(2/6)
BsaWI        1237  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     4695  TGTACA               T^GTACA
BspLU11I     1698  ACATGT               A^CATGT
BsrBI        3308  GAGCGG               CCGCTC(-3/-3)
BsrGI        4695  TGTACA               T^GTACA
BstEII       1704  GGTTACC              G^GT[ACGT]ACC
BstPI        1704  GGTTACC              G^GT[ACGT]ACC
CelII        2157  GCTTAGC              GC^T[ACGT]AGC
Eam1105I      100  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1704  GGTTACC              G^GT[ACGT]ACC
EcoRV        3454  GATATC               GAT^ATC
EcoT14I       663  CCATGG               C^C[AT][AT]GG
HgaI         1118  GACGC                GACGC(5/10)
NciI         3219  CCGGG                CC^[GC]GG
NcoI          663  CCATGG               C^CATGG
NheI          189  GCTAGC               G^CTAGC
NruI         4383  TCGCGA               TCG^CGA
PaeR7I       4528  CTCGAG               C^TCGAG
PstI         1899  CTGCAG               CTGCA^G
SnaBI         534  TACGTA               TAC^GTA
SphI          751  GCATGC               GCATG^C
SspBI        4695  TGTACA               T^GTACA
StyI          663  CCATGG               C^C[AT][AT]GG
SwaI         4839  ATTTAAAT             ATTT^AAAT
XbaI         4787  TCTAGA               T^CTAGA
XhoI         4528  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AflII         606  CTTAAG               C^TTAAG
             2854  CTTAAG              
AocI         2674  CCTGAGG              CC^T[ACGT]AGG
             3146  CCTGAGG             
AseI         3973  ATTAAT               AT^TAAT
             4760  ATTAAT              
AsnI         3973  ATTAAT               AT^TAAT
             4760  ATTAAT              
AspI         1844  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4207  GACGGGGTC           
AvaI         4283  CTCGGG               C^[CT]CG[GA]G
             4528  CTCGAG              
BalI         2119  TGGCCA               TGG^CCA
             2230  TGGCCA              
BamHI         893  GGATCC               G^GATCC
             2486  GGATCC              
BanI         1966  GGTGCC               G^G[CT][GA]CC
             4512  GGCACC              
BciVI         667  GGATAC               GTATCC(6/5)
             1788  GTATCC              
BfrI          606  CTTAAG               C^TTAAG
             2854  CTTAAG              
BsaAI         534  TACGTA               [CT]AC^GT[GA]
             2654  TACGTG              
BseRI        4656  CTCCTC               GAGGAG(10/8)
             2677  GAGGAG              
BsgI           95  GTGCAG               GTGCAG(16/14)
             1875  GTGCAG              
BsoBI        4283  CTCGGG               C^[CT]CG[GA]G
             4528  CTCGAG              
BsrDI         755  GCAATG               GCAATG(2/0)
              923  GCAATG              
Bsu36I       2674  CCTGAGG              CC^T[ACGT]AGG
             3146  CCTGAGG             
CfoI         2114  GCGC                 GCG^C
             3319  GCGC                
DsaI           58  CCGTGG               C^C[GA][CT]GG
              663  CCATGG              
EaeI         2119  TGGCCA               [CT]^GGCC[GA]
             2230  TGGCCA              
Eco81I       2674  CCTGAGG              CC^T[ACGT]AGG
             3146  CCTGAGG             
HapII        1238  CCGG                 C^CGG
             3219  CCGG                
HhaI         2114  GCGC                 GCG^C
             3319  GCGC                
HinP1I       2114  GCGC                 G^CGC
             3319  GCGC                
HpaII        1238  CCGG                 C^CGG
             3219  CCGG                
MluNI        2119  TGGCCA               TGG^CCA
             2230  TGGCCA              
MscI         2119  TGGCCA               TGG^CCA
             2230  TGGCCA              
MspA1I        296  CAGCTG               C[AC]G^C[GT]G
             3648  CAGCTG              
MspI         1238  CCGG                 C^CGG
             3219  CCGG                
NspI          751  GCATGC               [GA]CATG^[CT]
             1698  ACATGT              
PflFI        1844  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4207  GACGGGGTC           
PflMI         168  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4406  CCAACTCTTGG         
PshBI        3973  ATTAAT               AT^TAAT
             4760  ATTAAT              
PvuII         296  CAGCTG               CAG^CTG
             3648  CAGCTG              
SapI         3700  GAAGAGC              GCTCTTC(1/4)
             2953  GCTCTTC             
ScaI         3896  AGTACT               AGT^ACT
             4669  AGTACT              
StuI          938  AGGCCT               AGG^CCT
             1442  AGGCCT              
Tth111I      1844  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4207  GACGGGGTC           
Van91I        168  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4406  CCAACTCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       BbvI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    BspHI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRI       EcoRII      
EcoT22I     FauI        Fnu4HI      FokI        HaeIII      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MvnI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI