##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AgeI AscI AseI
AsnI AspI AvrII BamHI BbeI
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrGI BstBI
BstEII BstPI ClaI CpoI DraI
EcoO65I EcoRI EcoRV EcoT22I FseI
HpaI KasI KspI MamI MfeI
MluI MunI NarI NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1424 GGTACC G^GTACC
AccIII 2417 TCCGGA T^CCGGA
AflII 2434 CTTAAG C^TTAAG
AflIII 1835 ACGTGT A^C[GA][CT]GT
AhdI 2167 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1424 GGTACC G^GTACC
AspEI 2167 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3726 CCTCAGC CCTCAGC(-5/-2)
BciVI 2450 GGATAC GTATCC(6/5)
BclI 3341 TGATCA T^GATCA
BfrI 2434 CTTAAG C^TTAAG
BseAI 2417 TCCGGA T^CCGGA
BspEI 2417 TCCGGA T^CCGGA
BspHI 1451 TCATGA T^CATGA
BssHII 750 GCGCGC G^CGCGC
DrdI 2905 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2167 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3341 TGATCA T^GATCA
HincII 3253 GTCAAC GT[CT]^[GA]AC
HindII 3253 GTCAAC GT[CT]^[GA]AC
KpnI 1424 GGTACC GGTAC^C
MroI 2417 TCCGGA T^CCGGA
NruI 2361 TCGCGA TCG^CGA
PaeR7I 1984 CTCGAG C^TCGAG
RcaI 1451 TCATGA T^CATGA
SexAI 2069 ACCAGGT A^CC[AT]GGT
XbaI 3351 TCTAGA T^CTAGA
XhoI 1984 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 4143 GTATAC GT^[AC][GT]AC
4189 GTATAC
ApaI 1134 GGGCCC GGGCC^C
3898 GGGCCC
Asp700I 603 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAAAACTTTC
AviII 584 TGCGCA TGC^GCA
1229 TGCGCA
BcgI 3920 CGATGAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4441 GCAGTGGTCTCG
BglII 1265 AGATCT A^GATCT
2329 AGATCT
BsrDI 3129 CATTGC GCAATG(2/0)
3627 CATTGC
Bst1107I 4143 GTATAC GTA^TAC
4189 GTATAC
BstZ17I 4143 GTATAC GTA^TAC
4189 GTATAC
EcoNI 690 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3026 CCTCCCAGAGG
FspI 584 TGCGCA TGC^GCA
1229 TGCGCA
HindIII 2621 AAGCTT A^AGCTT
2894 AAGCTT
NdeI 4150 CATATG CA^TATG
4254 CATATG
SapI 2840 GAAGAGC GCTCTTC(1/4)
3411 GAAGAGC
SbfI 861 CCTGCAGG CCTGCA^GG
1995 CCTGCAGG
SfiI 834 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1113 GGCCGAGTCGGCC
SmaI 3099 CCCGGG CCC^GGG
3272 CCCGGG
Sse8387I 861 CCTGCAGG CCTGCA^GG
1995 CCTGCAGG
XmaCI 3099 CCCGGG C^CCGGG
3272 CCCGGG
XmaI 3099 CCCGGG C^CCGGG
3272 CCCGGG
XmnI 603 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII