##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AviII       
BamHI       BbeI        BbrPI       BcgI        BglII       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BstYI       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoT22I     FseI        FspI        HaeII       HpaI        
KasI        KspI        MflI        MluI        MroI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SpeI        Sse8387I    SspBI       SwaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII           3  CGCG                 CG^CG
AcyI         5243  GACGCC               G[GA]^CG[CT]C
AflIII       3362  ACATGT               A^C[GA][CT]GT
ApaI         4422  GGGCCC               GGGCC^C
Asp700I      3004  GAAAATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaAI        3046  TACGTA               [CT]AC^GT[GA]
BsaBI        3100  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        5243  GACGCC               G[GA]^CG[CT]C
BsaWI         762  ACCGGA               [AT]^CCGG[AT]
BspLU11I     3362  ACATGT               A^CATGT
BssSI        1973  CTCGTG               CACGAG(-5/-1)
Bst1107I     3049  GTATAC               GTA^TAC
BstUI           3  CGCG                 CG^CG
BstZ17I      3049  GTATAC               GTA^TAC
CfoI         5236  GCGC                 GCG^C
DraII        4421  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     4421  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         556  GATATC               GAT^ATC
HgaI         5243  GACGC                GACGC(5/10)
HhaI         5236  GCGC                 GCG^C
Hin1I        5243  GACGCC               G[GA]^CG[CT]C
HinP1I       5236  GCGC                 G^CGC
HincII       4201  GTCGAC               GT[CT]^[GA]AC
HindII       4201  GTCGAC               GT[CT]^[GA]AC
MamI         3100  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1891  CAATTG               C^AATTG
MunI         1891  CAATTG               C^AATTG
MvnI            3  CGCG                 CG^CG
PshAI        4194  GACCCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         4201  GTCGAC               G^TCGAC
SnaBI        3046  TACGTA               TAC^GTA
SphI         5232  GCATGC               GCATG^C
SspI         3007  AATATT               AAT^ATT
XbaI         2768  TCTAGA               T^CTAGA
XcmI         3490  CCAGGAAAACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         3004  GAAAATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AhdI         1627  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5534  GACAAGCAGTC         
AseI         2839  ATTAAT               AT^TAAT
             5013  ATTAAT              
AsnI         2839  ATTAAT               AT^TAAT
             5013  ATTAAT              
AspEI        1627  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5534  GACAAGCAGTC         
AvaI         3161  CTCGGG               C^[CT]CG[GA]G
             5211  CTCGGG              
BbvCI        5337  CCTCAGC              CCTCAGC(-5/-2)
             4268  GCTGAGG             
BclI         4320  TGATCA               T^GATCA
             4685  TGATCA              
BglI         2396  GCCTTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5207  GCCTCTCGGGC         
BlpI         4442  GCTCAGC              GC^T[ACGT]AGC
             4586  GCTCAGC             
Bpu1102I     4442  GCTCAGC              GC^T[ACGT]AGC
             4586  GCTCAGC             
BsmBI        1598  CGTCTC               CGTCTC(1/5)
             5273  GAGACG              
BsoBI        3161  CTCGGG               C^[CT]CG[GA]G
             5211  CTCGGG              
BsrDI        2147  CATTGC               GCAATG(2/0)
             5142  GCAATG              
BstAPI       2301  GCAAAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5166  GCAACCTCTGC         
BstBI         382  TTCGAA               TT^CGAA
              777  TTCGAA              
BstEII       1449  GGTGACC              G^GT[ACGT]ACC
             4610  GGTGACC             
BstPI        1449  GGTGACC              G^GT[ACGT]ACC
             4610  GGTGACC             
CelII        4442  GCTCAGC              GC^T[ACGT]AGC
             4586  GCTCAGC             
DsaI         3996  CCACGG               C^C[GA][CT]GG
             4606  CCACGG              
Eam1105I     1627  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5534  GACAAGCAGTC         
EcoNI        4553  CCTTGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5423  CCTACAGGAGG         
EcoO65I      1449  GGTGACC              G^GT[ACGT]ACC
             4610  GGTGACC             
EcoRI         647  GAATTC               G^AATTC
              716  GAATTC              
FbaI         4320  TGATCA               T^GATCA
             4685  TGATCA              
NdeI          921  CATATG               CA^TATG
             1987  CATATG              
NspV          382  TTCGAA               TT^CGAA
              777  TTCGAA              
PflMI         411  CCATACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4159  CCAGGAGTTGG         
PshBI        2839  ATTAAT               AT^TAAT
             5013  ATTAAT              
PstI         1787  CTGCAG               CTGCA^G
             4307  CTGCAG              
SapI          404  GAAGAGC              GCTCTTC(1/4)
             4034  GCTCTTC             
SfuI          382  TTCGAA               TT^CGAA
              777  TTCGAA              
StuI         1825  AGGCCT               AGG^CCT
             2403  AGGCCT              
Van91I        411  CCATACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4159  CCAGGAGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

Acc65I      AccI        AciI        AcsI        AfaI        
AflII       AluI        AlwI        AocI        ApoI        
Asp718I     AspHI       AspI        AvaII       AvrII       
BalI        BanI        BanII       BbsI        BbvI        
BciVI       BcnI        BfaI        BfrI        BlnI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       Bsu36I      Cac8I       Cfr13I      DdeI        
DpnII       DraI        EaeI        EarI        Eco81I      
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PleI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I