################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccIII AclI AgeI Alw44I AlwNI Aor51HI ApaLI AscI AviII BamHI BbeI BbrPI BcgI BglII BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BstYI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI HaeII HpaI KasI KspI MflI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I SspBI SwaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 3 CGCG CG^CG AcyI 5243 GACGCC G[GA]^CG[CT]C AflIII 3362 ACATGT A^C[GA][CT]GT ApaI 4422 GGGCCC GGGCC^C Asp700I 3004 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsaAI 3046 TACGTA [CT]AC^GT[GA] BsaBI 3100 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5243 GACGCC G[GA]^CG[CT]C BsaWI 762 ACCGGA [AT]^CCGG[AT] BspLU11I 3362 ACATGT A^CATGT BssSI 1973 CTCGTG CACGAG(-5/-1) Bst1107I 3049 GTATAC GTA^TAC BstUI 3 CGCG CG^CG BstZ17I 3049 GTATAC GTA^TAC CfoI 5236 GCGC GCG^C DraII 4421 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 4421 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 556 GATATC GAT^ATC HgaI 5243 GACGC GACGC(5/10) HhaI 5236 GCGC GCG^C Hin1I 5243 GACGCC G[GA]^CG[CT]C HinP1I 5236 GCGC G^CGC HincII 4201 GTCGAC GT[CT]^[GA]AC HindII 4201 GTCGAC GT[CT]^[GA]AC MamI 3100 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1891 CAATTG C^AATTG MunI 1891 CAATTG C^AATTG MvnI 3 CGCG CG^CG PshAI 4194 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 4201 GTCGAC G^TCGAC SnaBI 3046 TACGTA TAC^GTA SphI 5232 GCATGC GCATG^C SspI 3007 AATATT AAT^ATT XbaI 2768 TCTAGA T^CTAGA XcmI 3490 CCAGGAAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3004 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AhdI 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5534 GACAAGCAGTC AseI 2839 ATTAAT AT^TAAT 5013 ATTAAT AsnI 2839 ATTAAT AT^TAAT 5013 ATTAAT AspEI 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5534 GACAAGCAGTC AvaI 3161 CTCGGG C^[CT]CG[GA]G 5211 CTCGGG BbvCI 5337 CCTCAGC CCTCAGC(-5/-2) 4268 GCTGAGG BclI 4320 TGATCA T^GATCA 4685 TGATCA BglI 2396 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5207 GCCTCTCGGGC BlpI 4442 GCTCAGC GC^T[ACGT]AGC 4586 GCTCAGC Bpu1102I 4442 GCTCAGC GC^T[ACGT]AGC 4586 GCTCAGC BsmBI 1598 CGTCTC CGTCTC(1/5) 5273 GAGACG BsoBI 3161 CTCGGG C^[CT]CG[GA]G 5211 CTCGGG BsrDI 2147 CATTGC GCAATG(2/0) 5142 GCAATG BstAPI 2301 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5166 GCAACCTCTGC BstBI 382 TTCGAA TT^CGAA 777 TTCGAA BstEII 1449 GGTGACC G^GT[ACGT]ACC 4610 GGTGACC BstPI 1449 GGTGACC G^GT[ACGT]ACC 4610 GGTGACC CelII 4442 GCTCAGC GC^T[ACGT]AGC 4586 GCTCAGC DsaI 3996 CCACGG C^C[GA][CT]GG 4606 CCACGG Eam1105I 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5534 GACAAGCAGTC EcoNI 4553 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5423 CCTACAGGAGG EcoO65I 1449 GGTGACC G^GT[ACGT]ACC 4610 GGTGACC EcoRI 647 GAATTC G^AATTC 716 GAATTC FbaI 4320 TGATCA T^GATCA 4685 TGATCA NdeI 921 CATATG CA^TATG 1987 CATATG NspV 382 TTCGAA TT^CGAA 777 TTCGAA PflMI 411 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4159 CCAGGAGTTGG PshBI 2839 ATTAAT AT^TAAT 5013 ATTAAT PstI 1787 CTGCAG CTGCA^G 4307 CTGCAG SapI 404 GAAGAGC GCTCTTC(1/4) 4034 GCTCTTC SfuI 382 TTCGAA TT^CGAA 777 TTCGAA StuI 1825 AGGCCT AGG^CCT 2403 AGGCCT Van91I 411 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4159 CCAGGAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### Acc65I AccI AciI AcsI AfaI AflII AluI AlwI AocI ApoI Asp718I AspHI AspI AvaII AvrII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BfrI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I Cfr13I DdeI DpnII DraI EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I