################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AviII BamHI BbrPI BciVI BclI BfrI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmlI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 936 CGCG CG^CG ApaI 3955 GGGCCC GGGCC^C AspHI 100 GAGCTC G[AT]GC[AT]^C BalI 2932 TGGCCA TGG^CCA BbeI 4247 GGCGCC GGCGC^C BglI 4239 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1276 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1276 GCTTAGC GC^T[ACGT]AGC BsaAI 4161 TACGTG [CT]AC^GT[GA] BsaBI 4381 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 949 ACCGGA [AT]^CCGG[AT] BsiHKAI 100 GAGCTC G[AT]GC[AT]^C BsmBI 4127 CGTCTC CGTCTC(1/5) BspMI 30 ACCTGC ACCTGC(4/8) BstUI 936 CGCG CG^CG CelII 1276 GCTTAGC GC^T[ACGT]AGC DraIII 3326 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 3228 CCATGG C^C[GA][CT]GG EcoNI 2064 CCTTCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 4070 GCGGG CCCGC(4/6) HgaI 1103 GACGC GACGC(5/10) KasI 4247 GGCGCC G^GCGCC MamI 4381 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2932 TGGCCA TGG^CCA MscI 2932 TGGCCA TGG^CCA MvnI 936 CGCG CG^CG NarI 4247 GGCGCC GG^CGCC NcoI 3228 CCATGG C^CATGG PmeI 271 GTTTAAAC GTTT^AAAC PpuMI 4637 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 84 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 100 GAGCTC GAGCT^C SalI 1880 GTCGAC G^TCGAC ScaI 765 AGTACT AGT^ACT SfoI 4247 GGCGCC GGC^GCC SpeI 4827 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 1880 GTCGAC GT^[AC][GT]AC 4745 GTAGAC AcyI 1103 GACGCC G[GA]^CG[CT]C 4247 GGCGCC AflIII 2448 ACATGT A^C[GA][CT]GT 3193 ACATGT AspI 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4282 GACTCTGTC BanI 4247 GGCGCC G^G[CT][GA]CC 4480 GGTGCC BbvCI 3103 GCTGAGG CCTCAGC(-5/-2) 4191 GCTGAGG BcgI 1035 GCAGATGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4197 GCAGGAGAATCG BcnI 4021 CCGGG CC^[GC]GG 4154 CCGGG BsaHI 1103 GACGCC G[GA]^CG[CT]C 4247 GGCGCC BspLU11I 2448 ACATGT A^CATGT 3193 ACATGT BstXI 1453 CCAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4049 CCAGCACTTTGG Cac8I 214 GCTTGC GC[ACGT]^[ACGT]GC 1166 GCTTGC EaeI 530 CGGCCA [CT]^GGCC[GA] 2932 TGGCCA Hin1I 1103 GACGCC G[GA]^CG[CT]C 4247 GGCGCC HincII 1880 GTCGAC GT[CT]^[GA]AC 4109 GTCAAC HindII 1880 GTCGAC GT[CT]^[GA]AC 4109 GTCAAC HindIII 1594 AAGCTT A^AGCTT 1733 AAGCTT MaeII 1884 ACGT A^CGT 4162 ACGT NciI 4021 CCGGG CC^[GC]GG 4154 CCGGG PaeR7I 654 CTCGAG C^TCGAG 3826 CTCGAG PflFI 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4282 GACTCTGTC Tth111I 1175 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4282 GACTCTGTC XbaI 1638 TCTAGA T^CTAGA 1839 TCTAGA XhoI 654 CTCGAG C^TCGAG 3826 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AvaI AvaII AvrII BanII BbsI BbvI BfaI BglII BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I CfoI Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI