##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        Asp718I     AspEI       AviII       BamHI       
BbrPI       BciVI       BclI        BfrI        BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HpaI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflMI       PinAI       
PmaCI       PmlI        Psp1406I    PvuI        RcaI        
RsrII       SacII       SapI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    StuI        SwaI        Van91I      
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         936  CGCG                 CG^CG
ApaI         3955  GGGCCC               GGGCC^C
AspHI         100  GAGCTC               G[AT]GC[AT]^C
BalI         2932  TGGCCA               TGG^CCA
BbeI         4247  GGCGCC               GGCGC^C
BglI         4239  GCCGAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1276  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1276  GCTTAGC              GC^T[ACGT]AGC
BsaAI        4161  TACGTG               [CT]AC^GT[GA]
BsaBI        4381  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         949  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       100  GAGCTC               G[AT]GC[AT]^C
BsmBI        4127  CGTCTC               CGTCTC(1/5)
BspMI          30  ACCTGC               ACCTGC(4/8)
BstUI         936  CGCG                 CG^CG
CelII        1276  GCTTAGC              GC^T[ACGT]AGC
DraIII       3326  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3228  CCATGG               C^C[GA][CT]GG
EcoNI        2064  CCTTCTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         4070  GCGGG                CCCGC(4/6)
HgaI         1103  GACGC                GACGC(5/10)
KasI         4247  GGCGCC               G^GCGCC
MamI         4381  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2932  TGGCCA               TGG^CCA
MscI         2932  TGGCCA               TGG^CCA
MvnI          936  CGCG                 CG^CG
NarI         4247  GGCGCC               GG^CGCC
NcoI         3228  CCATGG               C^CATGG
PmeI          271  GTTTAAAC             GTTT^AAAC
PpuMI        4637  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI          84  GACTGTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          100  GAGCTC               GAGCT^C
SalI         1880  GTCGAC               G^TCGAC
ScaI          765  AGTACT               AGT^ACT
SfoI         4247  GGCGCC               GGC^GCC
SpeI         4827  ACTAGT               A^CTAGT


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1880  GTCGAC               GT^[AC][GT]AC
             4745  GTAGAC              
AcyI         1103  GACGCC               G[GA]^CG[CT]C
             4247  GGCGCC              
AflIII       2448  ACATGT               A^C[GA][CT]GT
             3193  ACATGT              
AspI         1175  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4282  GACTCTGTC           
BanI         4247  GGCGCC               G^G[CT][GA]CC
             4480  GGTGCC              
BbvCI        3103  GCTGAGG              CCTCAGC(-5/-2)
             4191  GCTGAGG             
BcgI         1035  GCAGATGTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4197  GCAGGAGAATCG        
BcnI         4021  CCGGG                CC^[GC]GG
             4154  CCGGG               
BsaHI        1103  GACGCC               G[GA]^CG[CT]C
             4247  GGCGCC              
BspLU11I     2448  ACATGT               A^CATGT
             3193  ACATGT              
BstXI        1453  CCAAGCCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4049  CCAGCACTTTGG        
Cac8I         214  GCTTGC               GC[ACGT]^[ACGT]GC
             1166  GCTTGC              
EaeI          530  CGGCCA               [CT]^GGCC[GA]
             2932  TGGCCA              
Hin1I        1103  GACGCC               G[GA]^CG[CT]C
             4247  GGCGCC              
HincII       1880  GTCGAC               GT[CT]^[GA]AC
             4109  GTCAAC              
HindII       1880  GTCGAC               GT[CT]^[GA]AC
             4109  GTCAAC              
HindIII      1594  AAGCTT               A^AGCTT
             1733  AAGCTT              
MaeII        1884  ACGT                 A^CGT
             4162  ACGT                
NciI         4021  CCGGG                CC^[GC]GG
             4154  CCGGG               
PaeR7I        654  CTCGAG               C^TCGAG
             3826  CTCGAG              
PflFI        1175  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4282  GACTCTGTC           
Tth111I      1175  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4282  GACTCTGTC           
XbaI         1638  TCTAGA               T^CTAGA
             1839  TCTAGA              
XhoI          654  CTCGAG               C^TCGAG
             3826  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
Asp700I     AvaI        AvaII       AvrII       BanII       
BbsI        BbvI        BfaI        BglII       BlnI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Bsu36I      CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmnI