##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AvrII BbeI BbrPI BclI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII BstBI ClaI CpoI
EagI EclXI Eco52I EcoRI FbaI
FseI HincII HindII HpaI KasI
MamI MluI MroI NarI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 788 GACGCC G[GA]^CG[CT]C
AflII 3881 CTTAAG C^TTAAG
AhdI 4467 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4378 ATTAAT AT^TAAT
AsnI 4378 ATTAAT AT^TAAT
AspEI 4467 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3629 CTCGGG C^[CT]CG[GA]G
BfrI 3881 CTTAAG C^TTAAG
BsaHI 788 GACGCC G[GA]^CG[CT]C
BsgI 3222 GTGCAG GTGCAG(16/14)
BsiEI 992 CGACCG CG[GA][CT]^CG
BsoBI 3629 CTCGGG C^[CT]CG[GA]G
BsrDI 4722 CATTGC GCAATG(2/0)
BsrFI 4763 GCCGGC [GA]^CCGG[CT]
Bst1107I 3836 GTATAC GTA^TAC
BstZ17I 3836 GTATAC GTA^TAC
Cfr10I 4763 GCCGGC [GA]^CCGG[CT]
DraI 4844 TTTAAA TTT^AAA
Eam1105I 4467 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1167 GATATC GAT^ATC
EcoT22I 252 ATGCAT ATGCA^T
HgaI 788 GACGC GACGC(5/10)
Hin1I 788 GACGCC G[GA]^CG[CT]C
KspI 9 CCGCGG CCGC^GG
NaeI 4763 GCCGGC GCC^GGC
NcoI 74 CCATGG C^CATGG
NdeI 3951 CATATG CA^TATG
NgoMIV 4763 GCCGGC G^CCGGC
NheI 947 GCTAGC G^CTAGC
NsiI 252 ATGCAT ATGCA^T
PshBI 4378 ATTAAT AT^TAAT
SacII 9 CCGCGG CCGC^GG
ScaI 4337 AGTACT AGT^ACT
SexAI 3421 ACCTGGT A^CC[AT]GGT
SspI 1693 AATATT AAT^ATT
XbaI 4476 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2993 GGTACC G^GTACC
3486 GGTACC
AccII 10 CGCG CG^CG
56 CGCG
Alw44I 366 GTGCAC G^TGCAC
1263 GTGCAC
Aor51HI 22 AGCGCT AGC^GCT
520 AGCGCT
ApaI 3011 GGGCCC GGGCC^C
3824 GGGCCC
ApaLI 366 GTGCAC G^TGCAC
1263 GTGCAC
Asp718I 2993 GGTACC G^GTACC
3486 GGTACC
BamHI 1058 GGATCC G^GATCC
4741 GGATCC
BbvCI 1972 CCTCAGC CCTCAGC(-5/-2)
2478 GCTGAGG
BcgI 2319 CGAAGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
703 GCACTCTACTCG
BlpI 2980 GCTTAGC GC^T[ACGT]AGC
4085 GCTAAGC
Bpu1102I 2980 GCTTAGC GC^T[ACGT]AGC
4085 GCTAAGC
BsmBI 434 CGTCTC CGTCTC(1/5)
786 GAGACG
BspHI 202 TCATGA T^CATGA
805 TCATGA
BspLU11I 1052 ACATGT A^CATGT
1897 ACATGT
BstUI 10 CGCG CG^CG
56 CGCG
BstXI 839 CCACACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1144 CCACAGGCCTGG
CelII 2980 GCTTAGC GC^T[ACGT]AGC
4085 GCTAAGC
DraIII 399 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4119 CACAAGGTG
DrdI 3723 GACATTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3762 GACTTCCGGGTC
Eco47III 22 AGCGCT AGC^GCT
520 AGCGCT
EcoNI 3452 CCTTAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3752 CCTGCAGAAGG
HindIII 3705 AAGCTT A^AGCTT
4753 AAGCTT
KpnI 2993 GGTACC GGTAC^C
3486 GGTACC
MfeI 2429 CAATTG C^AATTG
2678 CAATTG
MunI 2429 CAATTG C^AATTG
2678 CAATTG
MvnI 10 CGCG CG^CG
56 CGCG
PflMI 569 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3408 CCACAGATTGG
RcaI 202 TCATGA T^CATGA
805 TCATGA
SapI 345 GCTCTTC GCTCTTC(1/4)
2165 GCTCTTC
TaqI 2114 TCGA T^CGA
3652 TCGA
TthHB8I 2114 TCGA T^CGA
3652 TCGA
Van91I 569 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3408 CCACAGATTGG
XcmI 1501 CCATGAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1699 CCAAGCTAGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaII AviII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I XhoII XmnI