##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AviII BbrPI BcgI BciVI
BclI BsaAI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI KpnI KspI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 18 enzymes) #
###################################
AatII 3513 GACGTC GACGT^C
AccI 569 GTCTAC GT^[AC][GT]AC
AflIII 5066 ACGTGT A^C[GA][CT]GT
Aor51HI 2806 AGCGCT AGC^GCT
Asp700I 1776 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 4448 GGATCC G^GATCC
BsaBI 394 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 2987 CCGCTC CCGCTC(-3/-3)
BstAPI 4984 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 2806 AGCGCT AGC^GCT
HpaI 2204 GTTAAC GTT^AAC
MamI 394 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1991 GCTAGC G^CTAGC
PaeR7I 4418 CTCGAG C^TCGAG
PshAI 4018 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1319 AGTACT AGT^ACT
XhoI 4418 CTCGAG C^TCGAG
XmnI 1776 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflII 1361 CTTAAG C^TTAAG
4259 CTTAAG
BbsI 2646 GAAGAC GAAGAC(2/6)
3510 GAAGAC
BfrI 1361 CTTAAG C^TTAAG
4259 CTTAAG
BglII 393 AGATCT A^GATCT
498 AGATCT
BpuAI 2646 GAAGAC GAAGAC(2/6)
3510 GAAGAC
BsmBI 2935 CGTCTC CGTCTC(1/5)
782 GAGACG
EarI 1309 CTCTTC CTCTTC(1/4)
1431 CTCTTC
HgaI 300 GCGTC GACGC(5/10)
2541 GCGTC
HindIII 1709 AAGCTT A^AGCTT
4256 AAGCTT
Ksp632I 1309 CTCTTC CTCTTC(1/4)
1431 CTCTTC
MaeII 3514 ACGT A^CGT
5066 ACGT
NspI 1591 GCATGT [GA]CATG^[CT]
2493 ACATGC
SacI 523 GAGCTC GAGCT^C
1162 GAGCTC
SfiI 677 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2719 GGCCCTTTGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI