##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII Aor51HI AscI Asp718I AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BglI BlnI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 3175 CTTAAG C^TTAAG
AgeI 2669 ACCGGT A^CCGGT
AhdI 1751 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4922 GTGCAC G^TGCAC
AocI 1661 CCTTAGG CC^T[ACGT]AGG
ApaI 3803 GGGCCC GGGCC^C
ApaLI 4922 GTGCAC G^TGCAC
Asp700I 4692 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1751 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4922 GTGCAC G[AT]GC[AT]^C
BbvCI 4 CCTCAGC CCTCAGC(-5/-2)
BclI 1914 TGATCA T^GATCA
BfrI 3175 CTTAAG C^TTAAG
BglII 2203 AGATCT A^GATCT
BlpI 3783 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3783 GCTGAGC GC^T[ACGT]AGC
BsaAI 1818 CACGTA [CT]AC^GT[GA]
BsaBI 22 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2669 ACCGGT [AT]^CCGG[AT]
BseRI 1110 GAGGAG GAGGAG(10/8)
BsiHKAI 4922 GTGCAC G[AT]GC[AT]^C
BsrBI 3786 GAGCGG CCGCTC(-3/-3)
Bsu36I 1661 CCTTAGG CC^T[ACGT]AGG
CelII 3783 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1751 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4901 GAAGAG CTCTTC(1/4)
Eco81I 1661 CCTTAGG CC^T[ACGT]AGG
EcoRI 901 GAATTC G^AATTC
FbaI 1914 TGATCA T^GATCA
Ksp632I 4901 GAAGAG CTCTTC(1/4)
MamI 22 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1101 ACATGC [GA]CATG^[CT]
PinAI 2669 ACCGGT A^CCGGT
SfaNI 192 GCATC GCATC(5/9)
SmaI 4326 CCCGGG CCC^GGG
StuI 1186 AGGCCT AGG^CCT
XbaI 1336 TCTAGA T^CTAGA
XmaCI 4326 CCCGGG C^CCGGG
XmaI 4326 CCCGGG C^CCGGG
XmnI 4692 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 4346 CGCG CG^CG
4575 CGCG
AlwNI 1428 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2844 CAGACTCTG
AvaI 3775 CTCGGG C^[CT]CG[GA]G
4326 CCCGGG
BalI 859 TGGCCA TGG^CCA
2020 TGGCCA
BanI 2063 GGTGCC G^G[CT][GA]CC
4260 GGTGCC
BanII 3684 GAGCCC G[GA]GC[CT]^C
3803 GGGCCC
BsmBI 1713 GAGACG CGTCTC(1/5)
4472 GAGACG
BsmI 2154 GCATTC GAATGC(1/-1)
3055 GCATTC
BsoBI 3775 CTCGGG C^[CT]CG[GA]G
4326 CCCGGG
BspMI 3051 ACCTGC ACCTGC(4/8)
4529 ACCTGC
BsrFI 1870 GCCGGT [GA]^CCGG[CT]
2669 ACCGGT
Bst1107I 3987 GTATAC GTA^TAC
4720 GTATAC
BstUI 4346 CGCG CG^CG
4575 CGCG
BstYI 2203 AGATCT [GA]^GATC[CT]
4202 AGATCC
BstZ17I 3987 GTATAC GTA^TAC
4720 GTATAC
Cfr10I 1870 GCCGGT [GA]^CCGG[CT]
2669 ACCGGT
DraIII 1367 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2570 CACAAAGTG
EcoNI 1189 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4653 CCTGAAATAGG
HincII 2139 GTTGAC GT[CT]^[GA]AC
3198 GTTGAC
HindII 2139 GTTGAC GT[CT]^[GA]AC
3198 GTTGAC
MfeI 2677 CAATTG C^AATTG
4009 CAATTG
MflI 2203 AGATCT [GA]^GATC[CT]
4202 AGATCC
MluNI 859 TGGCCA TGG^CCA
2020 TGGCCA
MscI 859 TGGCCA TGG^CCA
2020 TGGCCA
MspA1I 2070 CAGCTG C[AC]G^C[GT]G
3769 CAGCTG
MunI 2677 CAATTG C^AATTG
4009 CAATTG
MvnI 4346 CGCG CG^CG
4575 CGCG
PshAI 3166 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4421 GACTGCAGTC
PvuII 2070 CAGCTG CAG^CTG
3769 CAGCTG
TaqI 177 TCGA T^CGA
2217 TCGA
TthHB8I 177 TCGA T^CGA
2217 TCGA
XcmI 1348 CCATTGGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2300 CCATAAAATCCTTGG
XhoII 2203 AGATCT [GA]^GATC[CT]
4202 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspI
AvaII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I