##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        ApoI        
AscI        AseI        AsnI        Asp700I     BfrI        
BsiWI       BspDI       Bst1107I    BstEII      BstPI       
BstZ17I     ClaI        DraI        DrdI        EcoO65I     
EcoRI       EcoT22I     FseI        NdeI        NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    SalI        SgrAI       
SnaBI       SpeI        SspI        SwaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccI         7074  GTAGAC               GT^[AC][GT]AC
AccIII       2280  TCCGGA               T^CCGGA
AhdI          332  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         332  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        9281  CCTAGG               C^CTAGG
BciVI       10754  GTATCC               GTATCC(6/5)
BlnI         9281  CCTAGG               C^CTAGG
BsaBI        7205  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2280  TCCGGA               T^CCGGA
BspEI        2280  TCCGGA               T^CCGGA
BspLU11I    10648  ACATGT               A^CATGT
BsrDI         481  CATTGC               GCAATG(2/0)
BstBI        4596  TTCGAA               TT^CGAA
Eam1105I      332  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        9171  GATATC               GAT^ATC
MamI         7205  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         8488  CAATTG               C^AATTG
MroI         2280  TCCGGA               T^CCGGA
MunI         8488  CAATTG               C^AATTG
NheI         6621  GCTAGC               G^CTAGC
NspV         4596  TTCGAA               TT^CGAA
PshAI        2269  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          110  CGATCG               CGAT^CG
SapI          452  GAAGAGC              GCTCTTC(1/4)
ScaI        10464  AGTACT               AGT^ACT
SfuI         4596  TTCGAA               TT^CGAA
XbaI         8087  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII        3823  GACGTC               GACGT^C
             5331  GACGTC              
Acc65I        863  GGTACC               G^GTACC
             4085  GGTACC              
Asp718I       863  GGTACC               G^GTACC
             4085  GGTACC              
AspI         1662  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
            10504  GACCAGGTC           
AviII        6142  TGCGCA               TGC^GCA
            10338  TGCGCA              
BglII        4376  AGATCT               A^GATCT
             7123  AGATCT              
BsaWI        1294  ACCGGA               [AT]^CCGG[AT]
             2280  TCCGGA              
BsmBI        5474  CGTCTC               CGTCTC(1/5)
             7601  CGTCTC              
BsmI         6647  GAATGC               GAATGC(1/-1)
             6724  GCATTC              
CpoI          105  CGGACCG              CG^G[AT]CCG
              131  CGGACCG             
EagI          241  CGGCCG               C^GGCCG
             5223  CGGCCG              
EclXI         241  CGGCCG               C^GGCCG
             5223  CGGCCG              
Eco52I        241  CGGCCG               C^GGCCG
             5223  CGGCCG              
FspI         6142  TGCGCA               TGC^GCA
            10338  TGCGCA              
HpaI         3183  GTTAAC               GTT^AAC
             5603  GTTAAC              
KpnI          863  GGTACC               GGTAC^C
             4085  GGTACC              
MluI         5060  ACGCGT               A^CGCGT
             5144  ACGCGT              
NcoI         2410  CCATGG               C^CATGG
             2558  CCATGG              
PflFI        1662  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
            10504  GACCAGGTC           
RsrII         105  CGGACCG              CG^G[AT]CCG
              131  CGGACCG             
SphI         4703  GCATGC               GCATG^C
             8594  GCATGC              
Tth111I      1662  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
            10504  GACCAGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  168 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BclI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco47III    Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        SacII       
Sau3AI      Sau96I      SbfI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SmaI        
SmlI        Sse8387I    SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI