################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp700I BfrI BsiWI BspDI Bst1107I BstEII BstPI BstZ17I ClaI DraI DrdI EcoO65I EcoRI EcoT22I FseI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I SalI SgrAI SnaBI SpeI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccI 7074 GTAGAC GT^[AC][GT]AC AccIII 2280 TCCGGA T^CCGGA AhdI 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 9281 CCTAGG C^CTAGG BciVI 10754 GTATCC GTATCC(6/5) BlnI 9281 CCTAGG C^CTAGG BsaBI 7205 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2280 TCCGGA T^CCGGA BspEI 2280 TCCGGA T^CCGGA BspLU11I 10648 ACATGT A^CATGT BsrDI 481 CATTGC GCAATG(2/0) BstBI 4596 TTCGAA TT^CGAA Eam1105I 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 9171 GATATC GAT^ATC MamI 7205 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 8488 CAATTG C^AATTG MroI 2280 TCCGGA T^CCGGA MunI 8488 CAATTG C^AATTG NheI 6621 GCTAGC G^CTAGC NspV 4596 TTCGAA TT^CGAA PshAI 2269 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 110 CGATCG CGAT^CG SapI 452 GAAGAGC GCTCTTC(1/4) ScaI 10464 AGTACT AGT^ACT SfuI 4596 TTCGAA TT^CGAA XbaI 8087 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 3823 GACGTC GACGT^C 5331 GACGTC Acc65I 863 GGTACC G^GTACC 4085 GGTACC Asp718I 863 GGTACC G^GTACC 4085 GGTACC AspI 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 10504 GACCAGGTC AviII 6142 TGCGCA TGC^GCA 10338 TGCGCA BglII 4376 AGATCT A^GATCT 7123 AGATCT BsaWI 1294 ACCGGA [AT]^CCGG[AT] 2280 TCCGGA BsmBI 5474 CGTCTC CGTCTC(1/5) 7601 CGTCTC BsmI 6647 GAATGC GAATGC(1/-1) 6724 GCATTC CpoI 105 CGGACCG CG^G[AT]CCG 131 CGGACCG EagI 241 CGGCCG C^GGCCG 5223 CGGCCG EclXI 241 CGGCCG C^GGCCG 5223 CGGCCG Eco52I 241 CGGCCG C^GGCCG 5223 CGGCCG FspI 6142 TGCGCA TGC^GCA 10338 TGCGCA HpaI 3183 GTTAAC GTT^AAC 5603 GTTAAC KpnI 863 GGTACC GGTAC^C 4085 GGTACC MluI 5060 ACGCGT A^CGCGT 5144 ACGCGT NcoI 2410 CCATGG C^CATGG 2558 CCATGG PflFI 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 10504 GACCAGGTC RsrII 105 CGGACCG CG^G[AT]CCG 131 CGGACCG SphI 4703 GCATGC GCATG^C 8594 GCATGC Tth111I 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 10504 GACCAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 168 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SacII Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SmlI Sse8387I SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI