##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbvCI BcgI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstBI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KspI MamI MluI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1117 GGTACC G^GTACC
AccII 2204 CGCG CG^CG
AccIII 1136 TCCGGA T^CCGGA
AclI 3606 AACGTT AA^CGTT
AflII 3460 CTTAAG C^TTAAG
AlwNI 1518 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1117 GGTACC G^GTACC
BalI 139 TGGCCA TGG^CCA
BamHI 1968 GGATCC G^GATCC
BbrPI 3723 CACGTG CAC^GTG
BclI 1734 TGATCA T^GATCA
BfrI 3460 CTTAAG C^TTAAG
BsaI 2362 GGTCTC GGTCTC(1/5)
BseAI 1136 TCCGGA T^CCGGA
BsgI 3444 CTGCAC GTGCAG(16/14)
BsmFI 4662 GTCCC GGGAC(10/14)
BsmI 516 GAATGC GAATGC(1/-1)
Bsp1407I 2426 TGTACA T^GTACA
BspEI 1136 TCCGGA T^CCGGA
BsrGI 2426 TGTACA T^GTACA
Bst1107I 3496 GTATAC GTA^TAC
BstEII 2500 GGTTACC G^GT[ACGT]ACC
BstPI 2500 GGTTACC G^GT[ACGT]ACC
BstUI 2204 CGCG CG^CG
BstZ17I 3496 GTATAC GTA^TAC
EaeI 139 TGGCCA [CT]^GGCC[GA]
EcoO65I 2500 GGTTACC G^GT[ACGT]ACC
EcoRV 819 GATATC GAT^ATC
FbaI 1734 TGATCA T^GATCA
KpnI 1117 GGTACC GGTAC^C
MluNI 139 TGGCCA TGG^CCA
MroI 1136 TCCGGA T^CCGGA
MscI 139 TGGCCA TGG^CCA
MvnI 2204 CGCG CG^CG
PflMI 2061 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3723 CACGTG CAC^GTG
PmlI 3723 CACGTG CAC^GTG
PshAI 200 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3606 AACGTT AA^CGTT
PvuII 469 CAGCTG CAG^CTG
SphI 2411 GCATGC GCATG^C
SspBI 2426 TGTACA T^GTACA
StuI 3359 AGGCCT AGG^CCT
Van91I 2061 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanI 1117 GGTACC G^G[CT][GA]CC
1478 GGTGCC
BanII 3183 GAGCTC G[GA]GC[CT]^C
4488 GAGCTC
BciVI 2335 GGATAC GTATCC(6/5)
680 GTATCC
BsaWI 1136 TCCGGA [AT]^CCGG[AT]
4280 TCCGGT
BseRI 4005 CTCCTC GAGGAG(10/8)
463 GAGGAG
BspLU11I 1427 ACATGT A^CATGT
1983 ACATGT
BspMI 485 GCAGGT ACCTGC(4/8)
2437 GCAGGT
BstXI 2352 CCAAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2917 CCACTTCTTTGG
DraII 738 AGGACCC [GA]G^G[ACGT]CC[CT]
4294 AGGTCCT
EcoNI 2288 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3423 CCTTACTAAGG
EcoO109I 738 AGGACCC [GA]G^G[ACGT]CC[CT]
4294 AGGTCCT
EcoT22I 2830 ATGCAT ATGCA^T
3534 ATGCAT
HapII 1137 CCGG C^CGG
4281 CCGG
HindIII 259 AAGCTT A^AGCTT
3472 AAGCTT
HpaI 1431 GTTAAC GTT^AAC
4221 GTTAAC
HpaII 1137 CCGG C^CGG
4281 CCGG
MfeI 1608 CAATTG C^AATTG
1675 CAATTG
MspA1I 469 CAGCTG C[AC]G^C[GT]G
674 CCGCTG
MspI 1137 CCGG C^CGG
4281 CCGG
MunI 1608 CAATTG C^AATTG
1675 CAATTG
NdeI 1374 CATATG CA^TATG
3502 CATATG
NsiI 2830 ATGCAT ATGCA^T
3534 ATGCAT
PpuMI 738 AGGACCC [GA]G^G[AT]CC[CT]
4294 AGGTCCT
SacI 3183 GAGCTC GAGCT^C
4488 GAGCTC
XbaI 1335 TCTAGA T^CTAGA
1694 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraIII EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII