##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcyI Aor51HI
AscI Asp700I BbeI BbrPI BcgI
BglI BglII BsaBI BsaHI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI HaeII Hin1I HincII
HindII HpaI KasI KspI MamI
MluI MroI NarI NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SpeI Sse8387I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 2995 AAATTT [GA]^AATT[CT]
AgeI 4021 ACCGGT A^CCGGT
Alw44I 4575 GTGCAC G^TGCAC
ApaLI 4575 GTGCAC G^TGCAC
ApoI 2995 AAATTT [GA]^AATT[CT]
AseI 2404 ATTAAT AT^TAAT
AsnI 2404 ATTAAT AT^TAAT
AviII 4246 TGCGCA TGC^GCA
BalI 313 TGGCCA TGG^CCA
BclI 4306 TGATCA T^GATCA
BsaAI 2751 TACGTA [CT]AC^GT[GA]
BsmBI 4188 CGTCTC CGTCTC(1/5)
Bsp1407I 1474 TGTACA T^GTACA
BsrGI 1474 TGTACA T^GTACA
BstAPI 4658 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1053 TTTAAA TTT^AAA
EaeI 313 TGGCCA [CT]^GGCC[GA]
FbaI 4306 TGATCA T^GATCA
FspI 4246 TGCGCA TGC^GCA
HindIII 4095 AAGCTT A^AGCTT
MfeI 2733 CAATTG C^AATTG
MluNI 313 TGGCCA TGG^CCA
MscI 313 TGGCCA TGG^CCA
MunI 2733 CAATTG C^AATTG
NcoI 1717 CCATGG C^CATGG
NdeI 1678 CATATG CA^TATG
PinAI 4021 ACCGGT A^CCGGT
PshAI 4846 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2404 ATTAAT AT^TAAT
SapI 2479 GCTCTTC GCTCTTC(1/4)
ScaI 3164 AGTACT AGT^ACT
SexAI 4603 ACCTGGT A^CC[AT]GGT
SmaI 4690 CCCGGG CCC^GGG
SnaBI 2751 TACGTA TAC^GTA
SspBI 1474 TGTACA T^GTACA
SspI 1725 AATATT AAT^ATT
StuI 4630 AGGCCT AGG^CCT
SwaI 1052 ATTTAAAT ATTT^AAAT
XmaCI 4690 CCCGGG C^CCGGG
XmaI 4690 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2417 GTAGAC GT^[AC][GT]AC
3884 GTAGAC
AccII 82 CGCG CG^CG
97 CGCG
AflII 1464 CTTAAG C^TTAAG
4131 CTTAAG
AocI 1217 CCTTAGG CC^T[ACGT]AGG
1800 CCTGAGG
ApaI 3603 GGGCCC GGGCC^C
4709 GGGCCC
BamHI 659 GGATCC G^GATCC
1664 GGATCC
BbvCI 2058 CCTCAGC CCTCAGC(-5/-2)
3980 CCTCAGC
BciVI 320 GGATAC GTATCC(6/5)
738 GTATCC
BfrI 1464 CTTAAG C^TTAAG
4131 CTTAAG
BspHI 2355 TCATGA T^CATGA
4676 TCATGA
BsrBI 4071 GAGCGG CCGCTC(-3/-3)
4272 GAGCGG
BssSI 608 CTCGTG CACGAG(-5/-1)
3319 CTCGTG
BstUI 82 CGCG CG^CG
97 CGCG
Bsu36I 1217 CCTTAGG CC^T[ACGT]AGG
1800 CCTGAGG
DraIII 3477 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4906 CACGGGGTG
DsaI 1717 CCATGG C^C[GA][CT]GG
4905 CCACGG
Eco81I 1217 CCTTAGG CC^T[ACGT]AGG
1800 CCTGAGG
EcoT22I 618 ATGCAT ATGCA^T
2153 ATGCAT
HgaI 4920 GACGC GACGC(5/10)
4187 GCGTC
MvnI 82 CGCG CG^CG
97 CGCG
NaeI 4397 GCCGGC GCC^GGC
4754 GCCGGC
NgoMIV 4397 GCCGGC G^CCGGC
4754 GCCGGC
NsiI 618 ATGCAT ATGCA^T
2153 ATGCAT
PflMI 3476 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3782 CCAGGGTCTGG
PpuMI 3724 AGGACCT [GA]G^G[AT]CC[CT]
4291 AGGACCT
RcaI 2355 TCATGA T^CATGA
4676 TCATGA
Van91I 3476 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3782 CCAGGGTCTGG
XcmI 1155 CCAGGATTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4798 CCATCATTGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
Acc65I AciI AfaI AflIII AhdI
AluI AlwI AlwNI Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BanI BanII BbsI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XhoII