##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        Alw44I      AlwNI       
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspI        AvaI        BcgI        BfrI        BglI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspEI       BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     CelII       CpoI        
DraIII      DrdI        EagI        EclXI       Eco52I      
EcoRV       FauI        FseI        KpnI        KspI        
MluI        MroI        MvnI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcyI          515  GGCGCC               G[GA]^CG[CT]C
AhdI         1231  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1343  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       830  AGCGCT               AGC^GCT
AspEI        1231  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3799  TGCGCA               TGC^GCA
BalI         3567  TGGCCA               TGG^CCA
BanII        3192  GGGCTC               G[GA]GC[CT]^C
BbeI          515  GGCGCC               GGCGC^C
BbrPI         656  CACGTG               CAC^GTG
BciVI        2127  GTATCC               GTATCC(6/5)
BcnI         1870  CCCGG                CC^[GC]GG
BglII        1812  AGATCT               A^GATCT
BsaBI         261  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         515  GGCGCC               G[GA]^CG[CT]C
BsmBI         454  GAGACG               CGTCTC(1/5)
BspDI         877  ATCGAT               AT^CGAT
BspHI         450  TCATGA               T^CATGA
BsrFI         486  GCCGGC               [GA]^CCGG[CT]
BstEII       3946  GGTGACC              G^GT[ACGT]ACC
BstPI        3946  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1343  CCTAAGG              CC^T[ACGT]AGG
Cfr10I        486  GCCGGC               [GA]^CCGG[CT]
ClaI          877  ATCGAT               AT^CGAT
EaeI         3567  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1231  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      830  AGCGCT               AGC^GCT
Eco81I       1343  CCTAAGG              CC^T[ACGT]AGG
EcoNI        2149  CCTAAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3946  GGTGACC              G^GT[ACGT]ACC
FspI         3799  TGCGCA               TGC^GCA
HgaI          456  GACGC                GACGC(5/10)
Hin1I         515  GGCGCC               G[GA]^CG[CT]C
HpaI           56  GTTAAC               GTT^AAC
KasI          515  GGCGCC               G^GCGCC
MamI          261  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2461  CAATTG               C^AATTG
MluNI        3567  TGGCCA               TGG^CCA
MscI         3567  TGGCCA               TGG^CCA
MspA1I       1350  CAGCTG               C[AC]G^C[GT]G
MunI         2461  CAATTG               C^AATTG
NaeI          486  GCCGGC               GCC^GGC
NarI          515  GGCGCC               GG^CGCC
NciI         1870  CCCGG                CC^[GC]GG
NgoMIV        486  GCCGGC               G^CCGGC
PmaCI         656  CACGTG               CAC^GTG
PmlI          656  CACGTG               CAC^GTG
PstI         2450  CTGCAG               CTGCA^G
PvuII        1350  CAGCTG               CAG^CTG
RcaI          450  TCATGA               T^CATGA
ScaI         2967  AGTACT               AGT^ACT
SfoI          515  GGCGCC               GGC^GCC
SphI         4552  GCATGC               GCATG^C
StuI         3932  AGGCCT               AGG^CCT
XcmI         4704  CCATGAAATATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          418  GCGG                 CCGC(-3/-1)
             1079  GCGG                
BamHI         266  GGATCC               G^GATCC
              316  GGATCC              
BbvCI         841  CCTCAGC              CCTCAGC(-5/-2)
             1352  GCTGAGG             
BsaAI         656  CACGTG               [CT]AC^GT[GA]
             1271  CACGTA              
BsgI         1512  GTGCAG               GTGCAG(16/14)
             4354  GTGCAG              
BsmI         2384  GCATTC               GAATGC(1/-1)
             3389  GCATTC              
BspLU11I     3322  ACATGT               A^CATGT
             3329  ACATGT              
BsrDI        3163  CATTGC               GCAATG(2/0)
             4223  CATTGC              
DraII         432  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1795  AGGGCCT             
EcoO109I      432  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1795  AGGGCCT             
EcoRI          67  GAATTC               G^AATTC
             3715  GAATTC              
HapII         487  CCGG                 C^CGG
             1871  CCGG                
HincII         56  GTTAAC               GT[CT]^[GA]AC
             1130  GTCAAC              
HindII         56  GTTAAC               GT[CT]^[GA]AC
             1130  GTCAAC              
HpaII         487  CCGG                 C^CGG
             1871  CCGG                
MspI          487  CCGG                 C^CGG
             1871  CCGG                
NcoI          523  CCATGG               C^CATGG
             2131  CCATGG              
NdeI          234  CATATG               CA^TATG
             2750  CATATG              
PflMI        1733  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3266  CCAAGCACTGG         
PshAI          99  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2026  GACTTTGGTC          
Van91I       1733  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3266  CCAAGCACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
ApoI        AseI        AsnI        AspHI       AvaII       
AvrII       BanI        BbsI        BbvI        BclI        
BfaI        BlnI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DsaI        EarI        EcoRII      
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HhaI        HinP1I      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PshBI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII