################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI Alw44I AlwNI ApaI ApaLI AscI Asp700I Asp718I AspI AvaI BcgI BfrI BglI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspEI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CpoI DraIII DrdI EagI EclXI Eco52I EcoRV FauI FseI KpnI KspI MluI MroI MvnI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 515 GGCGCC G[GA]^CG[CT]C AhdI 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1343 CCTAAGG CC^T[ACGT]AGG Aor51HI 830 AGCGCT AGC^GCT AspEI 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3799 TGCGCA TGC^GCA BalI 3567 TGGCCA TGG^CCA BanII 3192 GGGCTC G[GA]GC[CT]^C BbeI 515 GGCGCC GGCGC^C BbrPI 656 CACGTG CAC^GTG BciVI 2127 GTATCC GTATCC(6/5) BcnI 1870 CCCGG CC^[GC]GG BglII 1812 AGATCT A^GATCT BsaBI 261 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 515 GGCGCC G[GA]^CG[CT]C BsmBI 454 GAGACG CGTCTC(1/5) BspDI 877 ATCGAT AT^CGAT BspHI 450 TCATGA T^CATGA BsrFI 486 GCCGGC [GA]^CCGG[CT] BstEII 3946 GGTGACC G^GT[ACGT]ACC BstPI 3946 GGTGACC G^GT[ACGT]ACC Bsu36I 1343 CCTAAGG CC^T[ACGT]AGG Cfr10I 486 GCCGGC [GA]^CCGG[CT] ClaI 877 ATCGAT AT^CGAT EaeI 3567 TGGCCA [CT]^GGCC[GA] Eam1105I 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 830 AGCGCT AGC^GCT Eco81I 1343 CCTAAGG CC^T[ACGT]AGG EcoNI 2149 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3946 GGTGACC G^GT[ACGT]ACC FspI 3799 TGCGCA TGC^GCA HgaI 456 GACGC GACGC(5/10) Hin1I 515 GGCGCC G[GA]^CG[CT]C HpaI 56 GTTAAC GTT^AAC KasI 515 GGCGCC G^GCGCC MamI 261 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2461 CAATTG C^AATTG MluNI 3567 TGGCCA TGG^CCA MscI 3567 TGGCCA TGG^CCA MspA1I 1350 CAGCTG C[AC]G^C[GT]G MunI 2461 CAATTG C^AATTG NaeI 486 GCCGGC GCC^GGC NarI 515 GGCGCC GG^CGCC NciI 1870 CCCGG CC^[GC]GG NgoMIV 486 GCCGGC G^CCGGC PmaCI 656 CACGTG CAC^GTG PmlI 656 CACGTG CAC^GTG PstI 2450 CTGCAG CTGCA^G PvuII 1350 CAGCTG CAG^CTG RcaI 450 TCATGA T^CATGA ScaI 2967 AGTACT AGT^ACT SfoI 515 GGCGCC GGC^GCC SphI 4552 GCATGC GCATG^C StuI 3932 AGGCCT AGG^CCT XcmI 4704 CCATGAAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 418 GCGG CCGC(-3/-1) 1079 GCGG BamHI 266 GGATCC G^GATCC 316 GGATCC BbvCI 841 CCTCAGC CCTCAGC(-5/-2) 1352 GCTGAGG BsaAI 656 CACGTG [CT]AC^GT[GA] 1271 CACGTA BsgI 1512 GTGCAG GTGCAG(16/14) 4354 GTGCAG BsmI 2384 GCATTC GAATGC(1/-1) 3389 GCATTC BspLU11I 3322 ACATGT A^CATGT 3329 ACATGT BsrDI 3163 CATTGC GCAATG(2/0) 4223 CATTGC DraII 432 AGGGCCT [GA]G^G[ACGT]CC[CT] 1795 AGGGCCT EcoO109I 432 AGGGCCT [GA]G^G[ACGT]CC[CT] 1795 AGGGCCT EcoRI 67 GAATTC G^AATTC 3715 GAATTC HapII 487 CCGG C^CGG 1871 CCGG HincII 56 GTTAAC GT[CT]^[GA]AC 1130 GTCAAC HindII 56 GTTAAC GT[CT]^[GA]AC 1130 GTCAAC HpaII 487 CCGG C^CGG 1871 CCGG MspI 487 CCGG C^CGG 1871 CCGG NcoI 523 CCATGG C^CATGG 2131 CCATGG NdeI 234 CATATG CA^TATG 2750 CATATG PflMI 1733 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3266 CCAAGCACTGG PshAI 99 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2026 GACTTTGGTC Van91I 1733 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3266 CCAAGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI AvaII AvrII BanI BbsI BbvI BclI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII