##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
AscI AspEI AspI AvaI AviII
BamHI BbeI BbvCI BcgI BciVI
BglI BglII BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FauI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MamI MflI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2756 GGTACC G^GTACC
AciI 4199 CCGC CCGC(-3/-1)
AflII 595 CTTAAG C^TTAAG
Alw44I 3195 GTGCAC G^TGCAC
AlwNI 1148 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 673 GGGCCC GGGCC^C
ApaLI 3195 GTGCAC G^TGCAC
Asp718I 2756 GGTACC G^GTACC
AspHI 3195 GTGCAC G[AT]GC[AT]^C
AvrII 1638 CCTAGG C^CTAGG
BalI 2411 TGGCCA TGG^CCA
BbrPI 3326 CACGTG CAC^GTG
BcnI 3917 CCCGG CC^[GC]GG
BfrI 595 CTTAAG C^TTAAG
BlnI 1638 CCTAGG C^CTAGG
BpmI 275 CTCCAG CTGGAG(16/14)
BseRI 187 GAGGAG GAGGAG(10/8)
BsgI 3856 CTGCAC GTGCAG(16/14)
BsiHKAI 3195 GTGCAC G[AT]GC[AT]^C
BspHI 2390 TCATGA T^CATGA
BspMI 664 GCAGGT ACCTGC(4/8)
BstAPI 631 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2551 TTCGAA TT^CGAA
BstXI 2708 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2411 TGGCCA [CT]^GGCC[GA]
EcoNI 1677 CCTCTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3308 GAATTC G^AATTC
HaeII 120 AGCGCC [GA]GCGC^[CT]
HindIII 914 AAGCTT A^AGCTT
KpnI 2756 GGTACC GGTAC^C
MluNI 2411 TGGCCA TGG^CCA
MscI 2411 TGGCCA TGG^CCA
NciI 3917 CCCGG CC^[GC]GG
NspV 2551 TTCGAA TT^CGAA
PmaCI 3326 CACGTG CAC^GTG
PmlI 3326 CACGTG CAC^GTG
PshAI 1900 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 788 CTGCAG CTGCA^G
RcaI 2390 TCATGA T^CATGA
SfuI 2551 TTCGAA TT^CGAA
SpeI 2985 ACTAGT A^CTAGT
StuI 462 AGGCCT AGG^CCT
XcmI 1658 CCATTGCATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1407 GATCC GGATC(4/5)
762 GGATC
BanI 1763 GGTGCC G^G[CT][GA]CC
2756 GGTACC
BanII 673 GGGCCC G[GA]GC[CT]^C
1971 GGGCTC
BclI 735 TGATCA T^GATCA
4900 TGATCA
BsaAI 479 CACGTA [CT]AC^GT[GA]
3326 CACGTG
BsmAI 2469 GTCTC GTCTC(1/5)
3576 GTCTC
BsmFI 3010 GGGAC GGGAC(10/14)
456 GTCCC
Cac8I 1584 GCTGGC GC[ACGT]^[ACGT]GC
3105 GCCAGC
CfoI 121 GCGC GCG^C
4865 GCGC
DraII 1778 AGGTCCT [GA]G^G[ACGT]CC[CT]
3729 AGGTCCT
DraIII 968 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2495 CACATTGTG
EcoO109I 1778 AGGTCCT [GA]G^G[ACGT]CC[CT]
3729 AGGTCCT
EcoT22I 2649 ATGCAT ATGCA^T
4009 ATGCAT
FbaI 735 TGATCA T^GATCA
4900 TGATCA
HapII 1317 CCGG C^CGG
3918 CCGG
HhaI 121 GCGC GCG^C
4865 GCGC
HinP1I 121 GCGC G^CGC
4865 GCGC
HpaII 1317 CCGG C^CGG
3918 CCGG
MspA1I 2447 CAGCTG C[AC]G^C[GT]G
3614 CAGCTG
MspI 1317 CCGG C^CGG
3918 CCGG
NdeI 710 CATATG CA^TATG
1954 CATATG
NsiI 2649 ATGCAT ATGCA^T
4009 ATGCAT
PpuMI 1778 AGGTCCT [GA]G^G[AT]CC[CT]
3729 AGGTCCT
PvuII 2447 CAGCTG CAG^CTG
3614 CAGCTG
SspI 2438 AATATT AAT^ATT
4892 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AseI AsnI Asp700I AvaII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoRV
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MnlI
MseI MslI MunI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI