##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MamI MluI MluNI MroI MscI
MslI MvnI NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmlI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 453 GTCTAC GT^[AC][GT]AC
AcsI 2366 GAATTT [GA]^AATT[CT]
ApoI 2366 GAATTT [GA]^AATT[CT]
AspHI 2188 GAGCAC G[AT]GC[AT]^C
BlpI 1541 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1541 GCTTAGC GC^T[ACGT]AGC
BsaI 171 GGTCTC GGTCTC(1/5)
BsaWI 1454 ACCGGA [AT]^CCGG[AT]
BsiEI 4 CGGCCG CG[GA][CT]^CG
BsiHKAI 2188 GAGCAC G[AT]GC[AT]^C
BsmBI 2561 GAGACG CGTCTC(1/5)
BsmI 708 GCATTC GAATGC(1/-1)
BsrBI 1400 CCGCTC CCGCTC(-3/-3)
BssSI 2013 CTCGTG CACGAG(-5/-1)
CelII 1541 GCTTAGC GC^T[ACGT]AGC
EaeI 4 CGGCCG [CT]^GGCC[GA]
EagI 4 CGGCCG C^GGCCG
EclXI 4 CGGCCG C^GGCCG
Eco52I 4 CGGCCG C^GGCCG
EcoRV 1512 GATATC GAT^ATC
HaeII 391 AGCGCC [GA]GCGC^[CT]
HgaI 239 GACGC GACGC(5/10)
HindIII 1833 AAGCTT A^AGCTT
MaeII 344 ACGT A^CGT
NotI 3 GCGGCCGC GC^GGCCGC
PaeR7I 439 CTCGAG C^TCGAG
PmeI 1781 GTTTAAAC GTTT^AAAC
PshAI 3080 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 99 CCCGGG CCC^GGG
SmlI 439 CTCGAG C^T[CT][GA]AG
XbaI 1610 TCTAGA T^CTAGA
XcmI 1165 CCAAAGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 439 CTCGAG C^TCGAG
XmaCI 99 CCCGGG C^CCGGG
XmaI 99 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 627 CCTGAGG CC^T[ACGT]AGG
1950 CCTTAGG
Asp700I 2761 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2903 GAATAGTTTC
BclI 1708 TGATCA T^GATCA
2241 TGATCA
BglI 95 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1067 GCCCCAAGGGC
BspHI 2343 TCATGA T^CATGA
2608 TCATGA
BstAPI 321 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
873 GCATTAATTGC
BstF5I 2132 CATCC GGATG(2/0)
2586 GGATG
BstXI 788 CCAGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2632 CCAAAGTGCTGG
BstYI 1971 GGATCT [GA]^GATC[CT]
3058 GGATCT
Bsu36I 627 CCTGAGG CC^T[ACGT]AGG
1950 CCTTAGG
DraIII 1024 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1216 CACGGCGTG
DsaI 73 CCATGG C^C[GA][CT]GG
1984 CCATGG
Eco81I 627 CCTGAGG CC^T[ACGT]AGG
1950 CCTTAGG
EcoNI 755 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1468 CCTTGGAGAGG
FbaI 1708 TGATCA T^GATCA
2241 TGATCA
FokI 2132 CATCC GGATG(9/13)
2586 GGATG
HincII 2964 GTTAAC GT[CT]^[GA]AC
3172 GTTAAC
HindII 2964 GTTAAC GT[CT]^[GA]AC
3172 GTTAAC
HpaI 2964 GTTAAC GTT^AAC
3172 GTTAAC
MfeI 868 CAATTG C^AATTG
1521 CAATTG
MflI 1971 GGATCT [GA]^GATC[CT]
3058 GGATCT
MunI 868 CAATTG C^AATTG
1521 CAATTG
NcoI 73 CCATGG C^CATGG
1984 CCATGG
PpuMI 575 AGGTCCT [GA]G^G[AT]CC[CT]
779 GGGTCCC
PvuII 1173 CAGCTG CAG^CTG
1591 CAGCTG
RcaI 2343 TCATGA T^CATGA
2608 TCATGA
SexAI 1094 ACCAGGT A^CC[AT]GGT
1346 ACCTGGT
SspI 2292 AATATT AAT^ATT
2298 AATATT
TaqI 440 TCGA T^CGA
1577 TCGA
TthHB8I 440 TCGA T^CGA
1577 TCGA
XhoII 1971 GGATCT [GA]^GATC[CT]
3058 GGATCT
XmnI 2761 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2903 GAATAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AseI AsnI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I