##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp700I Asp718I AspEI
AviII AvrII BbrPI BbvCI BcgI
BciVI BlnI BlpI Bpu1102I BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspMI BstBI BstXI CelII ClaI
Eam1105I EcoNI EcoRV FseI FspI
HindIII KpnI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI SacI SalI
SbfI ScaI SfiI SfuI SmaI
SnaBI Sse8387I StuI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Alw44I 1478 GTGCAC G^TGCAC
AlwNI 790 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 644 CCTAAGG CC^T[ACGT]AGG
Aor51HI 34 AGCGCT AGC^GCT
ApaLI 1478 GTGCAC G^TGCAC
AspI 3478 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 679 CCCGAG C^[CT]CG[GA]G
BamHI 1589 GGATCC G^GATCC
BbeI 339 GGCGCC GGCGC^C
BsaAI 2065 TACGTG [CT]AC^GT[GA]
BsaBI 3464 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 893 GAGACC GGTCTC(1/5)
BsmBI 413 GAGACG CGTCTC(1/5)
BsoBI 679 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4050 TGTACA T^GTACA
BsrBI 275 GAGCGG CCGCTC(-3/-3)
BsrGI 4050 TGTACA T^GTACA
BssHII 635 GCGCGC G^CGCGC
Bst1107I 4330 GTATAC GTA^TAC
BstAPI 4120 GCATACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 359 GGTGACC G^GT[ACGT]ACC
BstPI 359 GGTGACC G^GT[ACGT]ACC
BstZ17I 4330 GTATAC GTA^TAC
Bsu36I 644 CCTAAGG CC^T[ACGT]AGG
CpoI 251 CGGTCCG CG^G[AT]CCG
DraIII 443 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 823 GACCGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 34 AGCGCT AGC^GCT
Eco81I 644 CCTAAGG CC^T[ACGT]AGG
EcoO65I 359 GGTGACC G^GT[ACGT]ACC
EcoRI 4363 GAATTC G^AATTC
EcoT22I 4118 ATGCAT ATGCA^T
KasI 339 GGCGCC G^GCGCC
MamI 3464 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 339 GGCGCC GG^CGCC
NsiI 4118 ATGCAT ATGCA^T
PflFI 3478 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1303 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1869 AGGTCCC [GA]G^G[AT]CC[CT]
RsrII 251 CGGTCCG CG^G[AT]CCG
SexAI 2754 ACCAGGT A^CC[AT]GGT
SfoI 339 GGCGCC GGC^GCC
SpeI 2266 ACTAGT A^CTAGT
SphI 836 GCATGC GCATG^C
SspBI 4050 TGTACA T^GTACA
Tth111I 3478 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1303 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2028 ACATGT A^C[GA][CT]GT
4426 ACATGT
AseI 1738 ATTAAT AT^TAAT
1998 ATTAAT
AsnI 1738 ATTAAT AT^TAAT
1998 ATTAAT
BclI 1851 TGATCA T^GATCA
4297 TGATCA
BglI 271 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4808 GCCCTGTTGGC
BglII 1816 AGATCT A^GATCT
2388 AGATCT
BpmI 3025 CTCCAG CTGGAG(16/14)
3966 CTCCAG
BseRI 576 GAGGAG GAGGAG(10/8)
672 GAGGAG
BsmI 1753 GAATGC GAATGC(1/-1)
4521 GCATTC
BspLU11I 2028 ACATGT A^CATGT
4426 ACATGT
BssSI 2888 CACGAG CACGAG(-5/-1)
2110 CTCGTG
EagI 309 CGGCCG C^GGCCG
728 CGGCCG
EclXI 309 CGGCCG C^GGCCG
728 CGGCCG
Eco52I 309 CGGCCG C^GGCCG
728 CGGCCG
FbaI 1851 TGATCA T^GATCA
4297 TGATCA
HpaI 2167 GTTAAC GTT^AAC
3405 GTTAAC
KspI 586 CCGCGG CCGC^GG
702 CCGCGG
NdeI 3311 CATATG CA^TATG
4752 CATATG
PshBI 1738 ATTAAT AT^TAAT
1998 ATTAAT
PvuII 1436 CAGCTG CAG^CTG
3665 CAGCTG
SacII 586 CCGCGG CCGC^GG
702 CCGCGG
SapI 2533 GAAGAGC GCTCTTC(1/4)
1633 GCTCTTC
SgrAI 341 CGCCGGCG C[GA]^CCGG[CT]G
602 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI AlwI ApaI
ApoI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BfrI BmyI BpuAI BsaHI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII