################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI Asp718I AspEI AvrII BbeI BciVI BfrI BglII BlnI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FseI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PvuI RsrII SalI SbfI ScaI SfoI SfuI SnaBI SpeI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 4727 AACGTT AA^CGTT AlwNI 579 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 4613 AGCGCT AGC^GCT ApaI 4184 GGGCCC GGGCC^C AseI 1234 ATTAAT AT^TAAT AsnI 1234 ATTAAT AT^TAAT Asp700I 1379 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 4280 TGGCCA TGG^CCA BamHI 616 GGATCC G^GATCC BbrPI 2801 CACGTG CAC^GTG BbvCI 1765 CCTCAGC CCTCAGC(-5/-2) BglI 4083 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 3519 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 3404 TCATGA T^CATGA BsrBI 1879 CCGCTC CCGCTC(-3/-3) BssHII 818 GCGCGC G^CGCGC BstAPI 2797 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1903 GGTGACC G^GT[ACGT]ACC BstPI 1903 GGTGACC G^GT[ACGT]ACC DraIII 2357 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4613 AGCGCT AGC^GCT EcoO65I 1903 GGTGACC G^GT[ACGT]ACC EcoRV 623 GATATC GAT^ATC HincII 932 GTCAAC GT[CT]^[GA]AC HindII 932 GTCAAC GT[CT]^[GA]AC HindIII 3764 AAGCTT A^AGCTT MamI 3519 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4280 TGGCCA TGG^CCA MscI 4280 TGGCCA TGG^CCA PaeR7I 2174 CTCGAG C^TCGAG PmaCI 2801 CACGTG CAC^GTG PmlI 2801 CACGTG CAC^GTG PshBI 1234 ATTAAT AT^TAAT Psp1406I 4727 AACGTT AA^CGTT RcaI 3404 TCATGA T^CATGA SapI 251 GAAGAGC GCTCTTC(1/4) SfiI 4082 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 2346 CGCCGGCG C[GA]^CCGG[CT]G SmaI 309 CCCGGG CCC^GGG SphI 3642 GCATGC GCATG^C SspI 4686 AATATT AAT^ATT XbaI 457 TCTAGA T^CTAGA XhoI 2174 CTCGAG C^TCGAG XmaCI 309 CCCGGG C^CCGGG XmaI 309 CCCGGG C^CCGGG XmnI 1379 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 2825 GTCTAC GT^[AC][GT]AC 3203 GTAGAC AcyI 2350 GGCGTC G[GA]^CG[CT]C 2701 GGCGTC AflIII 1653 ACGTGT A^C[GA][CT]GT 1776 ACGTGT AocI 1499 CCTCAGG CC^T[ACGT]AGG 3812 CCTCAGG AspI 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3982 GACCAGGTC AviII 804 TGCGCA TGC^GCA 2019 TGCGCA BcgI 76 CGAAGGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1637 GCAAGCCTATCG BclI 1143 TGATCA T^GATCA 3518 TGATCA BsaHI 2350 GGCGTC G[GA]^CG[CT]C 2701 GGCGTC BsmBI 1555 CGTCTC CGTCTC(1/5) 2046 GAGACG BssSI 1067 CACGAG CACGAG(-5/-1) 2405 CACGAG BstYI 616 GGATCC [GA]^GATC[CT] 1824 AGATCC Bsu36I 1499 CCTCAGG CC^T[ACGT]AGG 3812 CCTCAGG DraI 1246 TTTAAA TTT^AAA 1316 TTTAAA DrdI 950 GACGAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2471 GACATGCTCGTC Eco81I 1499 CCTCAGG CC^T[ACGT]AGG 3812 CCTCAGG EcoNI 947 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2311 CCTGCTGCAGG FbaI 1143 TGATCA T^GATCA 3518 TGATCA FspI 804 TGCGCA TGC^GCA 2019 TGCGCA Hin1I 2350 GGCGTC G[GA]^CG[CT]C 2701 GGCGTC KspI 2235 CCGCGG CCGC^GG 4535 CCGCGG MflI 616 GGATCC [GA]^GATC[CT] 1824 AGATCC NaeI 2347 GCCGGC GCC^GGC 2750 GCCGGC NgoMIV 2347 GCCGGC G^CCGGC 2750 GCCGGC PflFI 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3982 GACCAGGTC SacII 2235 CCGCGG CCGC^GG 4535 CCGCGG Tth111I 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3982 GACCAGGTC XcmI 3062 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3181 CCATGGGGGTAGTGG XhoII 616 GGATCC [GA]^GATC[CT] 1824 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I