##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AcsI        AflII       AgeI        
AhdI        Alw44I      ApaLI       ApoI        AscI        
Asp718I     AspEI       AvrII       BbeI        BciVI       
BfrI        BglII       BlnI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
EcoT22I     FseI        HpaI        KasI        KpnI        
MfeI        MluI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PvuI        
RsrII       SalI        SbfI        ScaI        SfoI        
SfuI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AclI         4727  AACGTT               AA^CGTT
AlwNI         579  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      4613  AGCGCT               AGC^GCT
ApaI         4184  GGGCCC               GGGCC^C
AseI         1234  ATTAAT               AT^TAAT
AsnI         1234  ATTAAT               AT^TAAT
Asp700I      1379  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         4280  TGGCCA               TGG^CCA
BamHI         616  GGATCC               G^GATCC
BbrPI        2801  CACGTG               CAC^GTG
BbvCI        1765  CCTCAGC              CCTCAGC(-5/-2)
BglI         4083  GCCAGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        3519  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI        3404  TCATGA               T^CATGA
BsrBI        1879  CCGCTC               CCGCTC(-3/-3)
BssHII        818  GCGCGC               G^CGCGC
BstAPI       2797  GCAGCACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1903  GGTGACC              G^GT[ACGT]ACC
BstPI        1903  GGTGACC              G^GT[ACGT]ACC
DraIII       2357  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     4613  AGCGCT               AGC^GCT
EcoO65I      1903  GGTGACC              G^GT[ACGT]ACC
EcoRV         623  GATATC               GAT^ATC
HincII        932  GTCAAC               GT[CT]^[GA]AC
HindII        932  GTCAAC               GT[CT]^[GA]AC
HindIII      3764  AAGCTT               A^AGCTT
MamI         3519  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        4280  TGGCCA               TGG^CCA
MscI         4280  TGGCCA               TGG^CCA
PaeR7I       2174  CTCGAG               C^TCGAG
PmaCI        2801  CACGTG               CAC^GTG
PmlI         2801  CACGTG               CAC^GTG
PshBI        1234  ATTAAT               AT^TAAT
Psp1406I     4727  AACGTT               AA^CGTT
RcaI         3404  TCATGA               T^CATGA
SapI          251  GAAGAGC              GCTCTTC(1/4)
SfiI         4082  GGCCAGCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2346  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          309  CCCGGG               CCC^GGG
SphI         3642  GCATGC               GCATG^C
SspI         4686  AATATT               AAT^ATT
XbaI          457  TCTAGA               T^CTAGA
XhoI         2174  CTCGAG               C^TCGAG
XmaCI         309  CCCGGG               C^CCGGG
XmaI          309  CCCGGG               C^CCGGG
XmnI         1379  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2825  GTCTAC               GT^[AC][GT]AC
             3203  GTAGAC              
AcyI         2350  GGCGTC               G[GA]^CG[CT]C
             2701  GGCGTC              
AflIII       1653  ACGTGT               A^C[GA][CT]GT
             1776  ACGTGT              
AocI         1499  CCTCAGG              CC^T[ACGT]AGG
             3812  CCTCAGG             
AspI         1361  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3982  GACCAGGTC           
AviII         804  TGCGCA               TGC^GCA
             2019  TGCGCA              
BcgI           76  CGAAGGAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1637  GCAAGCCTATCG        
BclI         1143  TGATCA               T^GATCA
             3518  TGATCA              
BsaHI        2350  GGCGTC               G[GA]^CG[CT]C
             2701  GGCGTC              
BsmBI        1555  CGTCTC               CGTCTC(1/5)
             2046  GAGACG              
BssSI        1067  CACGAG               CACGAG(-5/-1)
             2405  CACGAG              
BstYI         616  GGATCC               [GA]^GATC[CT]
             1824  AGATCC              
Bsu36I       1499  CCTCAGG              CC^T[ACGT]AGG
             3812  CCTCAGG             
DraI         1246  TTTAAA               TTT^AAA
             1316  TTTAAA              
DrdI          950  GACGAAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2471  GACATGCTCGTC        
Eco81I       1499  CCTCAGG              CC^T[ACGT]AGG
             3812  CCTCAGG             
EcoNI         947  CCTGACGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2311  CCTGCTGCAGG         
FbaI         1143  TGATCA               T^GATCA
             3518  TGATCA              
FspI          804  TGCGCA               TGC^GCA
             2019  TGCGCA              
Hin1I        2350  GGCGTC               G[GA]^CG[CT]C
             2701  GGCGTC              
KspI         2235  CCGCGG               CCGC^GG
             4535  CCGCGG              
MflI          616  GGATCC               [GA]^GATC[CT]
             1824  AGATCC              
NaeI         2347  GCCGGC               GCC^GGC
             2750  GCCGGC              
NgoMIV       2347  GCCGGC               G^CCGGC
             2750  GCCGGC              
PflFI        1361  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3982  GACCAGGTC           
SacII        2235  CCGCGG               CCGC^GG
             4535  CCGCGG              
Tth111I      1361  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3982  GACCAGGTC           
XcmI         3062  CCATGCTCCCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3181  CCATGGGGGTAGTGG     
XhoII         616  GGATCC               [GA]^GATC[CT]
             1824  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AccIII      AciI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MroI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I